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Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody

Lee, SangWook ; Kim, Soyoun ; Malm, Johan LU ; Jeong, Ok Chan ; Lilja, Hans LU orcid and Laurell, Thomas LU (2013) In Analytica Chimica Acta 796. p.108-114
Abstract
Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We... (More)
Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Antibody microarray, Porous silicon, Sandwich immunoassay, Prostate, specific antigen
in
Analytica Chimica Acta
volume
796
pages
108 - 114
publisher
Elsevier
external identifiers
  • wos:000324524200016
  • scopus:84883552552
  • pmid:24016590
ISSN
1873-4324
DOI
10.1016/j.aca.2013.06.041
language
English
LU publication?
yes
id
2bdbe307-ebff-4372-9c03-470aab60f480 (old id 4098911)
date added to LUP
2016-04-01 13:12:24
date last changed
2022-03-21 17:17:12
@article{2bdbe307-ebff-4372-9c03-470aab60f480,
  abstract     = {{Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved.}},
  author       = {{Lee, SangWook and Kim, Soyoun and Malm, Johan and Jeong, Ok Chan and Lilja, Hans and Laurell, Thomas}},
  issn         = {{1873-4324}},
  keywords     = {{Antibody microarray; Porous silicon; Sandwich immunoassay; Prostate; specific antigen}},
  language     = {{eng}},
  pages        = {{108--114}},
  publisher    = {{Elsevier}},
  series       = {{Analytica Chimica Acta}},
  title        = {{Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody}},
  url          = {{http://dx.doi.org/10.1016/j.aca.2013.06.041}},
  doi          = {{10.1016/j.aca.2013.06.041}},
  volume       = {{796}},
  year         = {{2013}},
}