Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody
(2013) In Analytica Chimica Acta 796. p.108-114- Abstract
- Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We... (More)
- Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4098911
- author
- Lee, SangWook ; Kim, Soyoun ; Malm, Johan LU ; Jeong, Ok Chan ; Lilja, Hans LU and Laurell, Thomas LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Antibody microarray, Porous silicon, Sandwich immunoassay, Prostate, specific antigen
- in
- Analytica Chimica Acta
- volume
- 796
- pages
- 108 - 114
- publisher
- Elsevier
- external identifiers
-
- wos:000324524200016
- scopus:84883552552
- pmid:24016590
- ISSN
- 1873-4324
- DOI
- 10.1016/j.aca.2013.06.041
- language
- English
- LU publication?
- yes
- id
- 2bdbe307-ebff-4372-9c03-470aab60f480 (old id 4098911)
- date added to LUP
- 2016-04-01 13:12:24
- date last changed
- 2022-03-21 17:17:12
@article{2bdbe307-ebff-4372-9c03-470aab60f480, abstract = {{Enriching the surface density of immobilized capture antibodies enhances the detection signal of antibody sandwich microarrays. In this study, we improved the detection sensitivity of our previously developed P-Si (porous silicon) antibody microarray by optimizing concentrations of the capturing antibody. We investigated immunoassays using a P-Si microarray at three different capture antibody (PSA - prostate specific antigen) concentrations, analyzing the influence of the antibody density on the assay detection sensitivity. The LOD (limit of detection) for PSA was 2.5 ng mL(-1), 80 pg mL(1), and 800 fg mL(-1) when arraying the PSA antibody, H117 at the concentration 15 mu g mL(-1), 35 mu g mL(-1), and 154 mu g mL(-1), respectively. We further investigated PSA spiked into human female serum in the range of 800 fg mL(-1) to 500 ng mL(-1). The microarray showed a LOD of 800 fg mL(-1) and a dynamic range of 800 fg mL(-1) to 80 ng mL(-1) in serum spiked samples. (C) 2013 Elsevier B.V. All rights reserved.}}, author = {{Lee, SangWook and Kim, Soyoun and Malm, Johan and Jeong, Ok Chan and Lilja, Hans and Laurell, Thomas}}, issn = {{1873-4324}}, keywords = {{Antibody microarray; Porous silicon; Sandwich immunoassay; Prostate; specific antigen}}, language = {{eng}}, pages = {{108--114}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{Improved porous silicon microarray based prostate specific antigen immunoassay by optimized surface density of the capture antibody}}, url = {{http://dx.doi.org/10.1016/j.aca.2013.06.041}}, doi = {{10.1016/j.aca.2013.06.041}}, volume = {{796}}, year = {{2013}}, }