Sub-micrometer morphology of human atherosclerotic plaque revealed by synchrotron radiation-based μCT—A comparison with histology
Truong, My LU
; Dreier, Till
LU
; Wasselius, Johan
LU
; Sundius, Lena
LU
; Persson, Ana
LU
; Lovric, Goran
; Bonnin, Anne
; Gonçalves, Isabel
LU
and Bech, Martin
LU
(2022)
In PLoS ONE
17(4).
p.1-18
- Abstract
- Histology is a long standing and well-established gold standard for pathological characterizations. In recent years however, synchrotron radiation-based micro-computed tomography (SRμCT) has become a tool for extending the imaging of two-dimensional thin sections into three-dimensional imaging of tissue blocks, enabling so-called virtual histology with arbitrary clipping planes, volumetric rendering and automatic segmentation. In this study, we present a thorough characterization of human carotid plaques after endarterectomy of patients with stroke or transient ischemic attack (TIA), investigating several different pathologic structures using both SRμCT and histology. Phase-contrast SRμCT was performed with two different magnifications... (More)
- Histology is a long standing and well-established gold standard for pathological characterizations. In recent years however, synchrotron radiation-based micro-computed tomography (SRμCT) has become a tool for extending the imaging of two-dimensional thin sections into three-dimensional imaging of tissue blocks, enabling so-called virtual histology with arbitrary clipping planes, volumetric rendering and automatic segmentation. In this study, we present a thorough characterization of human carotid plaques after endarterectomy of patients with stroke or transient ischemic attack (TIA), investigating several different pathologic structures using both SRμCT and histology. Phase-contrast SRμCT was performed with two different magnifications (voxel sizes 6.5 μm and 0.65 μm, respectively), and histology was performed with multiple different stainings (Alpha-actin, Glycophorin A, von Kossa, Movat, CD68). The 0.65 μm high-resolution SRμCT was performed on selected areas with plaque typical relevant morphology, identified on the 6.5 μm low-resolution SRμCT. The tomography datasets were reconstructed with additional 3D volume rendering and compared to histology. In total, nine different regions with typical pathologic structures were identified and imaged with high-resolution SRμCT. The results show many characteristics typical for advanced atherosclerotic plaques, clinically relevant, namely ruptures with thrombosis, neo-vascularization, inflammatory infiltrates in shoulder regions, lipid rich necrotic cores (LRNC), thin fibrous cap, calcifications, lumen irregularities, and changes in vessel wall structures such as the internal elastic membrane. This method’s non-destructive nature renders details of micro-structures with an excellent visual likeness to histology, with the additional strength of multiplanar and 3D visualization and the possibility of multiple re-scans. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2c09c956-24d9-44a2-af60-4731e08ccee2
- author
- Truong, My
LU
; Dreier, Till
LU
; Wasselius, Johan
LU
; Sundius, Lena
LU
; Persson, Ana
LU
; Lovric, Goran
; Bonnin, Anne
; Gonçalves, Isabel
LU
and Bech, Martin
LU
- organization
- publishing date
- 2022
- type
- Contribution to journal
- publication status
- published
- subject
- in
- PLoS ONE
- volume
- 17
- issue
- 4
- article number
- e0265598
- pages
- 1 - 18
- publisher
- Public Library of Science (PLoS)
- external identifiers
-
- pmid:35471989
- scopus:85128894482
- ISSN
- 1932-6203
- DOI
- 10.1371/journal.pone.0265598
- language
- English
- LU publication?
- yes
- id
- 2c09c956-24d9-44a2-af60-4731e08ccee2
- date added to LUP
- 2022-04-28 08:56:51
- date last changed
- 2022-07-04 14:06:49
@article{2c09c956-24d9-44a2-af60-4731e08ccee2,
abstract = {{Histology is a long standing and well-established gold standard for pathological characterizations. In recent years however, synchrotron radiation-based micro-computed tomography (SRμCT) has become a tool for extending the imaging of two-dimensional thin sections into three-dimensional imaging of tissue blocks, enabling so-called virtual histology with arbitrary clipping planes, volumetric rendering and automatic segmentation. In this study, we present a thorough characterization of human carotid plaques after endarterectomy of patients with stroke or transient ischemic attack (TIA), investigating several different pathologic structures using both SRμCT and histology. Phase-contrast SRμCT was performed with two different magnifications (voxel sizes 6.5 μm and 0.65 μm, respectively), and histology was performed with multiple different stainings (Alpha-actin, Glycophorin A, von Kossa, Movat, CD68). The 0.65 μm high-resolution SRμCT was performed on selected areas with plaque typical relevant morphology, identified on the 6.5 μm low-resolution SRμCT. The tomography datasets were reconstructed with additional 3D volume rendering and compared to histology. In total, nine different regions with typical pathologic structures were identified and imaged with high-resolution SRμCT. The results show many characteristics typical for advanced atherosclerotic plaques, clinically relevant, namely ruptures with thrombosis, neo-vascularization, inflammatory infiltrates in shoulder regions, lipid rich necrotic cores (LRNC), thin fibrous cap, calcifications, lumen irregularities, and changes in vessel wall structures such as the internal elastic membrane. This method’s non-destructive nature renders details of micro-structures with an excellent visual likeness to histology, with the additional strength of multiplanar and 3D visualization and the possibility of multiple re-scans.}},
author = {{Truong, My and Dreier, Till and Wasselius, Johan and Sundius, Lena and Persson, Ana and Lovric, Goran and Bonnin, Anne and Gonçalves, Isabel and Bech, Martin}},
issn = {{1932-6203}},
language = {{eng}},
number = {{4}},
pages = {{1--18}},
publisher = {{Public Library of Science (PLoS)}},
series = {{PLoS ONE}},
title = {{Sub-micrometer morphology of human atherosclerotic plaque revealed by synchrotron radiation-based μCT—A comparison with histology}},
url = {{http://dx.doi.org/10.1371/journal.pone.0265598}},
doi = {{10.1371/journal.pone.0265598}},
volume = {{17}},
year = {{2022}},
}