Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures
(2022) In Molecules 27(21).- Abstract
Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering,... (More)
Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.
(Less)
- author
- Giudice, Rita Del LU ; Paracini, Nicolò ; Laursen, Tomas ; Blanchet, Clement ; Roosen-Runge, Felix LU and Cárdenas, Marité LU
- publishing date
- 2022-11
- type
- Contribution to journal
- publication status
- published
- keywords
- bicelles, cryo-TEM, DLS, model membranes, SAXS
- in
- Molecules
- volume
- 27
- issue
- 21
- article number
- 7628
- publisher
- MDPI AG
- external identifiers
-
- pmid:36364455
- scopus:85141759223
- ISSN
- 1420-3049
- DOI
- 10.3390/molecules27217628
- language
- English
- LU publication?
- no
- id
- 2c7e91d9-3458-4537-a80d-6d41349690de
- date added to LUP
- 2023-08-14 10:28:28
- date last changed
- 2024-04-20 00:30:47
@article{2c7e91d9-3458-4537-a80d-6d41349690de,
abstract = {{<p>Bicelles are disk-shaped models of cellular membranes used to study lipid–protein interactions, as well as for structural and functional studies on transmembrane proteins. One challenge for the incorporation of transmembrane proteins in bicelles is the limited range of detergent and lipid combinations available for the successful reconstitution of proteins in model membranes. This is important, as the function and stability of transmembrane proteins are very closely linked to the detergents used for their purification and to the lipids that the proteins are embedded in. Here, we expand the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. We use a combination of dynamic light scattering, small-angle X-ray scattering and cryogenic electron microscopy to perform a systematic sample characterization, thus providing a set of conditions under which bicelles can be successfully formed.</p>}},
author = {{Giudice, Rita Del and Paracini, Nicolò and Laursen, Tomas and Blanchet, Clement and Roosen-Runge, Felix and Cárdenas, Marité}},
issn = {{1420-3049}},
keywords = {{bicelles; cryo-TEM; DLS; model membranes; SAXS}},
language = {{eng}},
number = {{21}},
publisher = {{MDPI AG}},
series = {{Molecules}},
title = {{Expanding the Toolbox for Bicelle-Forming Surfactant–Lipid Mixtures}},
url = {{http://dx.doi.org/10.3390/molecules27217628}},
doi = {{10.3390/molecules27217628}},
volume = {{27}},
year = {{2022}},
}