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High Diversity of Microcystin Chemotypes within a Summer Bloom of the Cyanobacterium Microcystis botrys

Johansson, Emma LU orcid ; Legrand, Catherine ; Björnerås, Caroline LU ; Godhe, Anna ; Mazur-Marzec, Hanna ; Säll, Torbjörn LU and Rengefors, Karin LU (2019) In Toxins 11(12).
Abstract
The fresh-water cyanobacterium Microcystis is known to form blooms world-wide, and is often responsible for the production of microcystins found in lake water. Microcystins are non-ribosomal peptides with toxic effects, e.g. on vertebrates, but their function remains largely unresolved. Moreover, not all strains produce microcystins, and many different microcystin variants have been described. Here we explored the diversity of microcystin variants within Microcystis botrys, a common bloom-former in Sweden. We isolated a total of 130 strains through the duration of a bloom in eutrophic Lake Vomb, and analyzed their microcystin profiles with tandem mass spectrometry (LC-MS/MS). We found that microcystin producing (28.5%) and non-producing... (More)
The fresh-water cyanobacterium Microcystis is known to form blooms world-wide, and is often responsible for the production of microcystins found in lake water. Microcystins are non-ribosomal peptides with toxic effects, e.g. on vertebrates, but their function remains largely unresolved. Moreover, not all strains produce microcystins, and many different microcystin variants have been described. Here we explored the diversity of microcystin variants within Microcystis botrys, a common bloom-former in Sweden. We isolated a total of 130 strains through the duration of a bloom in eutrophic Lake Vomb, and analyzed their microcystin profiles with tandem mass spectrometry (LC-MS/MS). We found that microcystin producing (28.5%) and non-producing (71.5%) M. botrys strains, co-existed throughout the bloom. However, microcystin producing strains were more prevalent towards the end of the sampling period. Overall, 26 unique M. botrys chemotypes were identified, and while some chemotypes re-occurred, others were found only once. The M. botrys chemotypes showed considerable variation both in terms of number of microcystin variants, as well as in what combinations the variants occurred. To our knowledge, this is the first report on microcystin chemotype variation and dynamics in M. botrys. In addition, our study verifies the co-existence of microcystin and non-microcystin producing strains, and we propose that environmental conditions may be implicated in determining their composition. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Toxins
volume
11
issue
12
article number
698
pages
16 pages
publisher
MDPI AG
external identifiers
  • scopus:85076286751
  • pmid:31805656
ISSN
2072-6651
DOI
10.3390/toxins11120698
language
English
LU publication?
yes
id
2d3e0e1f-52c8-44d5-9633-da3c861a3814
date added to LUP
2020-06-18 14:53:20
date last changed
2024-05-15 13:57:52
@article{2d3e0e1f-52c8-44d5-9633-da3c861a3814,
  abstract     = {{The fresh-water cyanobacterium Microcystis is known to form blooms world-wide, and is often responsible for the production of microcystins found in lake water. Microcystins are non-ribosomal peptides with toxic effects, e.g. on vertebrates, but their function remains largely unresolved. Moreover, not all strains produce microcystins, and many different microcystin variants have been described. Here we explored the diversity of microcystin variants within Microcystis botrys, a common bloom-former in Sweden. We isolated a total of 130 strains through the duration of a bloom in eutrophic Lake Vomb, and analyzed their microcystin profiles with tandem mass spectrometry (LC-MS/MS). We found that microcystin producing (28.5%) and non-producing (71.5%) M. botrys strains, co-existed throughout the bloom. However, microcystin producing strains were more prevalent towards the end of the sampling period. Overall, 26 unique M. botrys chemotypes were identified, and while some chemotypes re-occurred, others were found only once. The M. botrys chemotypes showed considerable variation both in terms of number of microcystin variants, as well as in what combinations the variants occurred. To our knowledge, this is the first report on microcystin chemotype variation and dynamics in M. botrys. In addition, our study verifies the co-existence of microcystin and non-microcystin producing strains, and we propose that environmental conditions may be implicated in determining their composition.}},
  author       = {{Johansson, Emma and Legrand, Catherine and Björnerås, Caroline and Godhe, Anna and Mazur-Marzec, Hanna and Säll, Torbjörn and Rengefors, Karin}},
  issn         = {{2072-6651}},
  language     = {{eng}},
  month        = {{12}},
  number       = {{12}},
  publisher    = {{MDPI AG}},
  series       = {{Toxins}},
  title        = {{High Diversity of Microcystin Chemotypes within a Summer Bloom of the Cyanobacterium Microcystis botrys}},
  url          = {{http://dx.doi.org/10.3390/toxins11120698}},
  doi          = {{10.3390/toxins11120698}},
  volume       = {{11}},
  year         = {{2019}},
}