Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development
(1999) In Journal of Biological Chemistry 274(15). p.10259-10267- Abstract
A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 κ promoter κY element as a bait. The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a κY element reporter gene... (More)
A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 κ promoter κY element as a bait. The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a κY element reporter gene upon co-transfection of HeLa cells. Spi-C RNA was expressed in mature B lymphocytes and at lower levels in macrophages. Furthermore, pre-B cell and plasma cell lines were Spi-C-negative, suggesting that Spi-C might be a regulatory molecule during a specific phase of B lymphoid development.
(Less)
- author
- Bemark, Mats LU ; Mårtensson, Annica LU ; Liberg, David LU and Leanderson, Tomas LU
- organization
- publishing date
- 1999-04-09
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Biological Chemistry
- volume
- 274
- issue
- 15
- pages
- 10259 - 10267
- publisher
- American Society for Biochemistry and Molecular Biology
- external identifiers
-
- pmid:10187812
- scopus:0039550799
- ISSN
- 0021-9258
- DOI
- 10.1074/jbc.274.15.10259
- language
- English
- LU publication?
- yes
- id
- 2d9fa33e-fc76-4261-afb1-672263537e0d
- date added to LUP
- 2023-12-06 16:49:14
- date last changed
- 2024-10-05 04:35:19
@article{2d9fa33e-fc76-4261-afb1-672263537e0d, abstract = {{<p>A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 κ promoter κY element as a bait. The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a κY element reporter gene upon co-transfection of HeLa cells. Spi-C RNA was expressed in mature B lymphocytes and at lower levels in macrophages. Furthermore, pre-B cell and plasma cell lines were Spi-C-negative, suggesting that Spi-C might be a regulatory molecule during a specific phase of B lymphoid development.</p>}}, author = {{Bemark, Mats and Mårtensson, Annica and Liberg, David and Leanderson, Tomas}}, issn = {{0021-9258}}, language = {{eng}}, month = {{04}}, number = {{15}}, pages = {{10259--10267}}, publisher = {{American Society for Biochemistry and Molecular Biology}}, series = {{Journal of Biological Chemistry}}, title = {{Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development}}, url = {{http://dx.doi.org/10.1074/jbc.274.15.10259}}, doi = {{10.1074/jbc.274.15.10259}}, volume = {{274}}, year = {{1999}}, }