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Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development

Bemark, Mats LU orcid ; Mårtensson, Annica LU ; Liberg, David LU and Leanderson, Tomas LU (1999) In Journal of Biological Chemistry 274(15). p.10259-10267
Abstract

A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 κ promoter κY element as a bait. The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a κY element reporter gene... (More)

A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 κ promoter κY element as a bait. The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a κY element reporter gene upon co-transfection of HeLa cells. Spi-C RNA was expressed in mature B lymphocytes and at lower levels in macrophages. Furthermore, pre-B cell and plasma cell lines were Spi-C-negative, suggesting that Spi-C might be a regulatory molecule during a specific phase of B lymphoid development.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
274
issue
15
pages
10259 - 10267
publisher
American Society for Biochemistry and Molecular Biology
external identifiers
  • pmid:10187812
  • scopus:0039550799
ISSN
0021-9258
DOI
10.1074/jbc.274.15.10259
language
English
LU publication?
yes
id
2d9fa33e-fc76-4261-afb1-672263537e0d
date added to LUP
2023-12-06 16:49:14
date last changed
2024-03-22 10:25:29
@article{2d9fa33e-fc76-4261-afb1-672263537e0d,
  abstract     = {{<p>A novel Ets protein was isolated by yeast one-hybrid screening of a cDNA library made from lipopolysaccharide-stimulated mouse splenic B cells, using the SP6 κ promoter κY element as a bait. The novel Ets protein was most closely related to PU.1 and Spi-B within the DNA binding Ets domain and was therefore named Spi-C. However, Spi-C may represent a novel subgroup within the Ets protein family, as it differed significantly from Spi-B and PU.1 within helix 1 of the Ets domain. Spi-C was encoded by a single-copy gene that was mapped to chromosome 10, region C. Spi-C interacted with DNA similarly to PU.1 as judged by methylation interference, band-shift and site selection analysis, and activated transcription of a κY element reporter gene upon co-transfection of HeLa cells. Spi-C RNA was expressed in mature B lymphocytes and at lower levels in macrophages. Furthermore, pre-B cell and plasma cell lines were Spi-C-negative, suggesting that Spi-C might be a regulatory molecule during a specific phase of B lymphoid development.</p>}},
  author       = {{Bemark, Mats and Mårtensson, Annica and Liberg, David and Leanderson, Tomas}},
  issn         = {{0021-9258}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{15}},
  pages        = {{10259--10267}},
  publisher    = {{American Society for Biochemistry and Molecular Biology}},
  series       = {{Journal of Biological Chemistry}},
  title        = {{Spi-C, a novel Ets protein that is temporally regulated during B lymphocyte development}},
  url          = {{http://dx.doi.org/10.1074/jbc.274.15.10259}},
  doi          = {{10.1074/jbc.274.15.10259}},
  volume       = {{274}},
  year         = {{1999}},
}