Yeast-bacteria competition induced new metabolic traits through large-scale genomic rearrangements in Lachancea kluyveri
(2017) In FEMS Yeast Research 17(6).- Abstract
Large-scale chromosomal rearrangements are an important source of evolutionary novelty that may have reshaped the genomes of existing yeast species. They dramatically alter genome organization and gene expression fueling a phenotypic leap in response to environmental constraints. Although the emergence of such signatures of genetic diversity is thought to be associated with human exploitation of yeasts, less is known about the driving forces operating in natural habitats. Here we hypothesize that an ecological battlefield characteristic of every autumn when fruits ripen accounts for the genomic innovations in natural populations. We described a long-term cross-kingdom competition experiment between Lachancea kluyveri and five species of... (More)
Large-scale chromosomal rearrangements are an important source of evolutionary novelty that may have reshaped the genomes of existing yeast species. They dramatically alter genome organization and gene expression fueling a phenotypic leap in response to environmental constraints. Although the emergence of such signatures of genetic diversity is thought to be associated with human exploitation of yeasts, less is known about the driving forces operating in natural habitats. Here we hypothesize that an ecological battlefield characteristic of every autumn when fruits ripen accounts for the genomic innovations in natural populations. We described a long-term cross-kingdom competition experiment between Lachancea kluyveri and five species of bacteria. Now, we report how we further subjected the same yeast to a sixth species of bacteria, Pseudomonas fluorescens, resulting in the appearance of a fixed and stably inherited large-scale genomic rearrangement in two out of three parallel evolution lines. The 'extra-banded' karyotype, characterized by a higher fitness and an elevated fermentative capacity, conferred the emergence of new metabolic traits in most carbon sources and osmolytes. We tracked down the event to a duplication and translocation event involving a 261-kb segment. Such an experimental setup described here is an attractive method for developing industrial strains without genetic engineering strategies.
(Less)
- author
- Zhou, Nerve LU ; Bottagisi, Samuele ; Katz, Michael LU ; Schacherer, Joseph ; Friedrich, Anne ; Gojkovic, Zoran ; Swamy, Krishna B.S. ; Knecht, Wolfgang LU ; Compagno, Concetta and Piškur, Jure LU
- organization
- publishing date
- 2017-09-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Experimental evolution, Genome evolution, Large-scale genomic rearrangements, Strain development, Yeast-bacteria co-evolution
- in
- FEMS Yeast Research
- volume
- 17
- issue
- 6
- article number
- fox060
- publisher
- Oxford University Press
- external identifiers
-
- pmid:28910985
- wos:000412838700007
- scopus:85037524388
- ISSN
- 1567-1356
- DOI
- 10.1093/femsyr/fox060
- language
- English
- LU publication?
- yes
- id
- 2df5e2d7-dd35-4f0a-8a78-ca6d020d0037
- date added to LUP
- 2018-01-02 10:52:28
- date last changed
- 2024-05-13 01:04:31
@article{2df5e2d7-dd35-4f0a-8a78-ca6d020d0037,
abstract = {{<p>Large-scale chromosomal rearrangements are an important source of evolutionary novelty that may have reshaped the genomes of existing yeast species. They dramatically alter genome organization and gene expression fueling a phenotypic leap in response to environmental constraints. Although the emergence of such signatures of genetic diversity is thought to be associated with human exploitation of yeasts, less is known about the driving forces operating in natural habitats. Here we hypothesize that an ecological battlefield characteristic of every autumn when fruits ripen accounts for the genomic innovations in natural populations. We described a long-term cross-kingdom competition experiment between Lachancea kluyveri and five species of bacteria. Now, we report how we further subjected the same yeast to a sixth species of bacteria, Pseudomonas fluorescens, resulting in the appearance of a fixed and stably inherited large-scale genomic rearrangement in two out of three parallel evolution lines. The 'extra-banded' karyotype, characterized by a higher fitness and an elevated fermentative capacity, conferred the emergence of new metabolic traits in most carbon sources and osmolytes. We tracked down the event to a duplication and translocation event involving a 261-kb segment. Such an experimental setup described here is an attractive method for developing industrial strains without genetic engineering strategies.</p>}},
author = {{Zhou, Nerve and Bottagisi, Samuele and Katz, Michael and Schacherer, Joseph and Friedrich, Anne and Gojkovic, Zoran and Swamy, Krishna B.S. and Knecht, Wolfgang and Compagno, Concetta and Piškur, Jure}},
issn = {{1567-1356}},
keywords = {{Experimental evolution; Genome evolution; Large-scale genomic rearrangements; Strain development; Yeast-bacteria co-evolution}},
language = {{eng}},
month = {{09}},
number = {{6}},
publisher = {{Oxford University Press}},
series = {{FEMS Yeast Research}},
title = {{Yeast-bacteria competition induced new metabolic traits through large-scale genomic rearrangements in Lachancea kluyveri}},
url = {{http://dx.doi.org/10.1093/femsyr/fox060}},
doi = {{10.1093/femsyr/fox060}},
volume = {{17}},
year = {{2017}},
}