Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans

Martinez, Alejandra LU ; Delgado, Osvaldo LU ; Baigori, M D and Sineriz, F (2005) In Biotechnology Letters 27(8). p.545-550
Abstract
The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).
Please use this url to cite or link to this publication:
author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
purification, Bacillus halodurans MIR32, over-expression, sequence, xylanase
in
Biotechnology Letters
volume
27
issue
8
pages
545 - 550
publisher
Springer Science and Business Media B.V.
external identifiers
  • pmid:15973487
  • wos:000231955300003
  • scopus:20544471907
ISSN
1573-6776
DOI
10.1007/s10529-005-2879-2
language
English
LU publication?
yes
id
2ecb9584-4441-4fe5-8fbc-1b0c1d91141a (old id 224637)
date added to LUP
2016-04-01 16:11:25
date last changed
2025-10-14 09:37:52
@article{2ecb9584-4441-4fe5-8fbc-1b0c1d91141a,
  abstract     = {{The BhMIR32 xyn11A gene, encoding an extracellular endoxylanase of potential interest in bio-bleaching applications, was amplified from Bacillus halodurans MIR32 genomic DNA. The protein encoded is an endo-1,4-beta-xylanase belonging to family 11 of glycosyl hydrolases. Its nucleotide sequence was analysed and the mature peptide was subcloned into pET22b(+) expression vector. The enzyme was over-expressed in a high density Escherichia coli culture as a soluble and active protein, and purified in a single step by immobilised metal ion affinity chromatography with a specific activity of 3073 IU mg(-1).}},
  author       = {{Martinez, Alejandra and Delgado, Osvaldo and Baigori, M D and Sineriz, F}},
  issn         = {{1573-6776}},
  keywords     = {{purification; Bacillus halodurans MIR32; over-expression; sequence; xylanase}},
  language     = {{eng}},
  number       = {{8}},
  pages        = {{545--550}},
  publisher    = {{Springer Science and Business Media B.V.}},
  series       = {{Biotechnology Letters}},
  title        = {{Sequence analysis, cloning and over-expression of an endoxylanase from the alkaliphilic Bacillus halodurans}},
  url          = {{http://dx.doi.org/10.1007/s10529-005-2879-2}},
  doi          = {{10.1007/s10529-005-2879-2}},
  volume       = {{27}},
  year         = {{2005}},
}