Structural studies of hydrated samples of amorphous calcium phosphate and phosphoprotein nanoclusters.
(2016) In European Biophysics Journal- Abstract
- There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by... (More)
- There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by the complementary X-ray and FTIR data, collected on the same samples. The absence of a distinct diffraction pattern from the nanoclusters supports the generally accepted amorphous calcium phosphate structure of the mineral core. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/8577137
- author
- Lenton, Samuel ; Nylander, Tommy LU ; Holt, Carl ; Sawyer, Lindsay ; Härtlein, Michael ; Müller, Harrald and Teixeira, Susana C M
- organization
- publishing date
- 2016-01-16
- type
- Contribution to journal
- publication status
- published
- subject
- in
- European Biophysics Journal
- publisher
- Springer
- external identifiers
-
- pmid:26780236
- scopus:84954456210
- pmid:26780236
- wos:000378049100003
- ISSN
- 1432-1017
- DOI
- 10.1007/s00249-015-1109-7
- language
- English
- LU publication?
- yes
- id
- 2efc164a-d529-4351-babb-f1291afbb254 (old id 8577137)
- date added to LUP
- 2016-04-01 13:17:14
- date last changed
- 2025-04-04 14:23:16
@article{2efc164a-d529-4351-babb-f1291afbb254,
abstract = {{There are abundant examples of nanoclusters and inorganic microcrystals in biology. Their study under physiologically relevant conditions remains challenging due to their heterogeneity, instability, and the requirements of sample preparation. Advantages of using neutron diffraction and contrast matching to characterize biomaterials are highlighted in this article. We have applied these and complementary techniques to search for nanocrystals within clusters of calcium phosphate sequestered by bovine phosphopeptides, derived from osteopontin or casein. The neutron diffraction patterns show broad features that could be consistent with hexagonal hydroxyapatite crystallites smaller than 18.9 Å. Such nanocrystallites are, however, undetected by the complementary X-ray and FTIR data, collected on the same samples. The absence of a distinct diffraction pattern from the nanoclusters supports the generally accepted amorphous calcium phosphate structure of the mineral core.}},
author = {{Lenton, Samuel and Nylander, Tommy and Holt, Carl and Sawyer, Lindsay and Härtlein, Michael and Müller, Harrald and Teixeira, Susana C M}},
issn = {{1432-1017}},
language = {{eng}},
month = {{01}},
publisher = {{Springer}},
series = {{European Biophysics Journal}},
title = {{Structural studies of hydrated samples of amorphous calcium phosphate and phosphoprotein nanoclusters.}},
url = {{http://dx.doi.org/10.1007/s00249-015-1109-7}},
doi = {{10.1007/s00249-015-1109-7}},
year = {{2016}},
}