Identification of Haemophilus influenzae type b isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry
Månsson, Viktor LU ; Resman, Fredrik LU ; Kostrzewa, Markus ; Nilson, Bo LU
and Riesbeck, Kristian
LU
(2015)
In Journal of Clinical Microbiology
53(7).
p.2215-2224
- Abstract
- Haemophilus influenzae type b (Hib) is, in contrast to non-b H. influenzae, associated with severe invasive disease such as meningitis and epiglottitis in small children. To date accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. MALDI-TOF MS provides rapid bacterial diagnostics and is increasingly used in clinical microbiology laboratories. Here MALDI-TOF MS was evaluated as a novel approach to separate Hib from other H. influenzae. PCR-verified Hib and non-Hib reference isolates were selected based on genetic and spectral characteristics. Mass spectra of reference isolates were acquired, and used to generate different classification algorithms for Hib/non-Hib separation using both ClinProTools and... (More)
- Haemophilus influenzae type b (Hib) is, in contrast to non-b H. influenzae, associated with severe invasive disease such as meningitis and epiglottitis in small children. To date accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. MALDI-TOF MS provides rapid bacterial diagnostics and is increasingly used in clinical microbiology laboratories. Here MALDI-TOF MS was evaluated as a novel approach to separate Hib from other H. influenzae. PCR-verified Hib and non-Hib reference isolates were selected based on genetic and spectral characteristics. Mass spectra of reference isolates were acquired, and used to generate different classification algorithms for Hib/non-Hib separation using both ClinProTools and MALDI Biotyper software. A test series of mass spectra from 33 Hib and 77 non-Hib isolates, all characterized by PCR, was used to evaluate the algorithms. Several algorithms yielded good results but the two best were a ClinProTools model based on 22 separating peaks and a subtyping main spectra (MSP) model using MALDI Biotyper. The ClinProTools model had a sensitivity of 100%, a specificity of 99% and the results were 98% reproducible using a different MALDI-TOF MS instrument. The Biotyper subtyping MSPs had a sensitivity of 97%, a specificity of 100% and 93% reproducibility. Our results suggest that it is possible to use MALDI-TOF MS to differentiate Hib from other H. influenzae. This is a promising method to rapidly identify Hib in unvaccinated populations, and for screening or surveillance of Hib carriage in vaccinated populations. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/5461498
- author
- Månsson, Viktor
LU
; Resman, Fredrik
LU
; Kostrzewa, Markus
; Nilson, Bo
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Clinical Microbiology
- volume
- 53
- issue
- 7
- pages
- 2215 - 2224
- publisher
- American Society for Microbiology
- external identifiers
-
- pmid:25926500
- wos:000358287700030
- scopus:84932635228
- pmid:25926500
- ISSN
- 1098-660X
- DOI
- 10.1128/JCM.00137-15
- language
- English
- LU publication?
- yes
- id
- 2f11c190-35d2-4edd-9a27-9809cade2792 (old id 5461498)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/25926500?dopt=Abstract
- date added to LUP
- 2016-04-01 09:56:17
- date last changed
- 2023-03-16 23:40:19
@article{2f11c190-35d2-4edd-9a27-9809cade2792,
abstract = {{Haemophilus influenzae type b (Hib) is, in contrast to non-b H. influenzae, associated with severe invasive disease such as meningitis and epiglottitis in small children. To date accurate H. influenzae capsule typing requires PCR, a time-consuming and cumbersome method. MALDI-TOF MS provides rapid bacterial diagnostics and is increasingly used in clinical microbiology laboratories. Here MALDI-TOF MS was evaluated as a novel approach to separate Hib from other H. influenzae. PCR-verified Hib and non-Hib reference isolates were selected based on genetic and spectral characteristics. Mass spectra of reference isolates were acquired, and used to generate different classification algorithms for Hib/non-Hib separation using both ClinProTools and MALDI Biotyper software. A test series of mass spectra from 33 Hib and 77 non-Hib isolates, all characterized by PCR, was used to evaluate the algorithms. Several algorithms yielded good results but the two best were a ClinProTools model based on 22 separating peaks and a subtyping main spectra (MSP) model using MALDI Biotyper. The ClinProTools model had a sensitivity of 100%, a specificity of 99% and the results were 98% reproducible using a different MALDI-TOF MS instrument. The Biotyper subtyping MSPs had a sensitivity of 97%, a specificity of 100% and 93% reproducibility. Our results suggest that it is possible to use MALDI-TOF MS to differentiate Hib from other H. influenzae. This is a promising method to rapidly identify Hib in unvaccinated populations, and for screening or surveillance of Hib carriage in vaccinated populations.}},
author = {{Månsson, Viktor and Resman, Fredrik and Kostrzewa, Markus and Nilson, Bo and Riesbeck, Kristian}},
issn = {{1098-660X}},
language = {{eng}},
number = {{7}},
pages = {{2215--2224}},
publisher = {{American Society for Microbiology}},
series = {{Journal of Clinical Microbiology}},
title = {{Identification of Haemophilus influenzae type b isolates by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry}},
url = {{http://dx.doi.org/10.1128/JCM.00137-15}},
doi = {{10.1128/JCM.00137-15}},
volume = {{53}},
year = {{2015}},
}