Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism
(2021) In Nature Communications 12(1).- Abstract
Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the... (More)
Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.
(Less)
- author
- organization
- publishing date
- 2021-02-17
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Diet, Forkhead Transcription Factors/immunology, Homeostasis/immunology, Interleukin-17/immunology, Intestine, Large/immunology, Intestine, Small/immunology, Male, Methylococcus capsulatus/chemistry, Mice, Inbred C57BL, Microbiota/immunology, Nuclear Receptor Subfamily 1, Group F, Member 3/immunology, Obesity/immunology, Proteins/immunology, T-Lymphocytes, Regulatory/immunology
- in
- Nature Communications
- volume
- 12
- issue
- 1
- article number
- 1093
- publisher
- Nature Publishing Group
- external identifiers
-
- scopus:85101092638
- pmid:33597537
- ISSN
- 2041-1723
- DOI
- 10.1038/s41467-021-21408-9
- language
- English
- LU publication?
- yes
- additional info
- These authors jointly supervised this work: Benjamin A. H. Jensen, Karsten Kristiansen, Tor E. Lea.
- id
- 2f33c9a6-540f-4eba-a9c3-139a17b4de63
- date added to LUP
- 2021-03-26 09:48:53
- date last changed
- 2024-09-07 16:28:52
@article{2f33c9a6-540f-4eba-a9c3-139a17b4de63, abstract = {{<p>Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.</p>}}, author = {{Jensen, Benjamin A H and Holm, Jacob B and Larsen, Ida S and von Burg, Nicole and Derer, Stefanie and Sonne, Si B and Pærregaard, Simone I and Damgaard, Mads V and Indrelid, Stine A and Rivollier, Aymeric and Agrinier, Anne-Laure and Sulek, Karolina and Arnoldussen, Yke J and Fjære, Even and Marette, André and Angell, Inga L and Rudi, Knut and Treebak, Jonas T and Madsen, Lise and Åkesson, Caroline Piercey and Agace, William and Sina, Christian and Kleiveland, Charlotte R and Kristiansen, Karsten and Lea, Tor E}}, issn = {{2041-1723}}, keywords = {{Animals; Diet; Forkhead Transcription Factors/immunology; Homeostasis/immunology; Interleukin-17/immunology; Intestine, Large/immunology; Intestine, Small/immunology; Male; Methylococcus capsulatus/chemistry; Mice, Inbred C57BL; Microbiota/immunology; Nuclear Receptor Subfamily 1, Group F, Member 3/immunology; Obesity/immunology; Proteins/immunology; T-Lymphocytes, Regulatory/immunology}}, language = {{eng}}, month = {{02}}, number = {{1}}, publisher = {{Nature Publishing Group}}, series = {{Nature Communications}}, title = {{Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism}}, url = {{http://dx.doi.org/10.1038/s41467-021-21408-9}}, doi = {{10.1038/s41467-021-21408-9}}, volume = {{12}}, year = {{2021}}, }