Monocyte depletion early after stroke promotes neurogenesis from endogenous neural stem cells in adult brain
Laterza, Cecilia LU ; Wattananit, Somsak LU ; Uoshima, Naomi ; Ge, Ruimin LU ; Pekny, Roy ; Tornero, Daniel LU ; Monni, Emanuela LU ; Lindvall, Olle LU and Kokaia, Zaal LU
(2017)
In Experimental Neurology
297.
p.129-137
- Abstract
Ischemic stroke, caused by middle cerebral artery occlusion, leads to long-lasting formation of new striatal neurons from neural stem/progenitor cells (NSPCs) in the subventricular zone (SVZ) of adult rodents. Concomitantly with this neurogenic response, SVZ exhibits activation of resident microglia and infiltrating monocytes. Here we show that depletion of circulating monocytes, using the anti-CCR2 antibody MC-21 during the first week after stroke, enhances striatal neurogenesis at one week post-insult, most likely by increasing short-term survival of the newly formed neuroblasts in the SVZ and adjacent striatum. Blocking monocyte recruitment did not alter the volume of the ischemic lesion but gave rise to reduced astrocyte activation... (More)
Ischemic stroke, caused by middle cerebral artery occlusion, leads to long-lasting formation of new striatal neurons from neural stem/progenitor cells (NSPCs) in the subventricular zone (SVZ) of adult rodents. Concomitantly with this neurogenic response, SVZ exhibits activation of resident microglia and infiltrating monocytes. Here we show that depletion of circulating monocytes, using the anti-CCR2 antibody MC-21 during the first week after stroke, enhances striatal neurogenesis at one week post-insult, most likely by increasing short-term survival of the newly formed neuroblasts in the SVZ and adjacent striatum. Blocking monocyte recruitment did not alter the volume of the ischemic lesion but gave rise to reduced astrocyte activation in SVZ and adjacent striatum, which could contribute to the improved neuroblast survival. A similar decrease of astrocyte activation was found in and around human induced pluripotent stem cell (iPSC)-derived NSPCs transplanted into striatum at one week after stroke in monocyte-depleted mice. However, there was no effect on neurogenesis in the graft as determined 8 weeks after implantation. Our findings demonstrate, for the first time, that a specific cellular component of the early inflammatory reaction in SVZ and adjacent striatum following stroke, i.e., infiltrating monocytes, compromises the short-term neurogenic response neurogenesis from endogenous NSPCs.
(Less)
- author
- Laterza, Cecilia
LU
; Wattananit, Somsak
LU
; Uoshima, Naomi
; Ge, Ruimin
LU
; Pekny, Roy
; Tornero, Daniel
LU
; Monni, Emanuela
LU
; Lindvall, Olle
LU
and Kokaia, Zaal
LU
- organization
- publishing date
- 2017-11-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Monocyte depletion, Neurogenesis, Reactive gliosis
- in
- Experimental Neurology
- volume
- 297
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:28746827
- wos:000412617300013
- scopus:85027497828
- ISSN
- 0014-4886
- DOI
- 10.1016/j.expneurol.2017.07.012
- language
- English
- LU publication?
- yes
- id
- 2fa54e51-b96a-4d1b-8809-d6423ab5fdee
- date added to LUP
- 2017-09-05 14:46:15
- date last changed
- 2024-01-14 04:44:15
@article{2fa54e51-b96a-4d1b-8809-d6423ab5fdee,
abstract = {{<p>Ischemic stroke, caused by middle cerebral artery occlusion, leads to long-lasting formation of new striatal neurons from neural stem/progenitor cells (NSPCs) in the subventricular zone (SVZ) of adult rodents. Concomitantly with this neurogenic response, SVZ exhibits activation of resident microglia and infiltrating monocytes. Here we show that depletion of circulating monocytes, using the anti-CCR2 antibody MC-21 during the first week after stroke, enhances striatal neurogenesis at one week post-insult, most likely by increasing short-term survival of the newly formed neuroblasts in the SVZ and adjacent striatum. Blocking monocyte recruitment did not alter the volume of the ischemic lesion but gave rise to reduced astrocyte activation in SVZ and adjacent striatum, which could contribute to the improved neuroblast survival. A similar decrease of astrocyte activation was found in and around human induced pluripotent stem cell (iPSC)-derived NSPCs transplanted into striatum at one week after stroke in monocyte-depleted mice. However, there was no effect on neurogenesis in the graft as determined 8 weeks after implantation. Our findings demonstrate, for the first time, that a specific cellular component of the early inflammatory reaction in SVZ and adjacent striatum following stroke, i.e., infiltrating monocytes, compromises the short-term neurogenic response neurogenesis from endogenous NSPCs.</p>}},
author = {{Laterza, Cecilia and Wattananit, Somsak and Uoshima, Naomi and Ge, Ruimin and Pekny, Roy and Tornero, Daniel and Monni, Emanuela and Lindvall, Olle and Kokaia, Zaal}},
issn = {{0014-4886}},
keywords = {{Monocyte depletion; Neurogenesis; Reactive gliosis}},
language = {{eng}},
month = {{11}},
pages = {{129--137}},
publisher = {{Elsevier}},
series = {{Experimental Neurology}},
title = {{Monocyte depletion early after stroke promotes neurogenesis from endogenous neural stem cells in adult brain}},
url = {{http://dx.doi.org/10.1016/j.expneurol.2017.07.012}},
doi = {{10.1016/j.expneurol.2017.07.012}},
volume = {{297}},
year = {{2017}},
}