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Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury

Williams, Jonathan A. LU ; Huesa, Carmen ; Turunen, Mikael J. LU ; Oo, James A. ; Radzins, Oskars LU ; Gardner, Wilf ; Windmill, James F.C. ; Isaksson, Hanna LU orcid ; Tanner, K. Elizabeth LU and Riddell, John S. , et al. (2022) In Journal of Musculoskeletal Neuronal Interactions 22(2). p.212-234
Abstract

Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10-or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy. Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV... (More)

Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10-or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy. Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6-and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Bone Mechanical Properties, Fourier-transform Infrared Spectroscopy, microCT, Osteoporosis, Spinal Cord Transection
in
Journal of Musculoskeletal Neuronal Interactions
volume
22
issue
2
pages
23 pages
publisher
International Society of Musculoskeletal and Neuronal Interactions
external identifiers
  • pmid:35642701
  • scopus:85131091955
ISSN
1108-7161
language
English
LU publication?
yes
id
2fcee091-ba74-40f6-a5ed-61c14d31ced5
date added to LUP
2023-01-03 12:32:43
date last changed
2025-04-04 21:58:28
@article{2fcee091-ba74-40f6-a5ed-61c14d31ced5,
  abstract     = {{<p>Objective: Characterise the spatiotemporal trabecular and cortical bone responses to complete spinal cord injury (SCI) in young rats. Methods: 8-week-old male Wistar rats received T9-transection SCI and were euthanised 2-, 6-, 10-or 16-weeks post-surgery. Outcome measures were assessed using micro-computed tomography, mechanical testing, serum markers and Fourier-transform infrared spectroscopy. Results: The trabecular and cortical bone responses to SCI are site-specific. Metaphyseal trabecular BV/TV was 59% lower, characterised by fewer and thinner trabeculae at 2-weeks post-SCI, while epiphyseal BV/TV was 23% lower with maintained connectivity. At later-time points, metaphyseal BV/TV remained unchanged, while epiphyseal BV/TV increased. The total area of metaphyseal and mid-diaphyseal cortical bone were lower from 2-weeks and between 6-and 10-weeks post-SCI, respectively. This suggested that SCI-induced bone changes observed in the rat model were not solely attributable to bone loss, but also to suppressed bone growth. No tissue mineral density differences were observed at any time-point, suggesting that decreased whole-bone mechanical properties were primarily the result of changes to the spatial distribution of bone. Conclusion: Young SCI rat trabecular bone changes resemble those observed clinically in adult and paediatric SCI, while cortical bone changes resemble paediatric SCI only.</p>}},
  author       = {{Williams, Jonathan A. and Huesa, Carmen and Turunen, Mikael J. and Oo, James A. and Radzins, Oskars and Gardner, Wilf and Windmill, James F.C. and Isaksson, Hanna and Tanner, K. Elizabeth and Riddell, John S. and Coupaud, Sylvie}},
  issn         = {{1108-7161}},
  keywords     = {{Bone Mechanical Properties; Fourier-transform Infrared Spectroscopy; microCT; Osteoporosis; Spinal Cord Transection}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{212--234}},
  publisher    = {{International Society of Musculoskeletal and Neuronal Interactions}},
  series       = {{Journal of Musculoskeletal Neuronal Interactions}},
  title        = {{Time course changes to structural, mechanical and material properties of bone in rats after complete spinal cord injury}},
  volume       = {{22}},
  year         = {{2022}},
}