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Combining gas-phase electrophoretic mobility molecular analysis (GEMMA), light scattering, field flow fractionation and cryo electron microscopy in a multidimensional approach to characterize liposomal carrier vesicles

Urey, Carlos LU ; Weiss, Victor U.; Gondikas, Andreas; von der Kammer, Frank; Hofmann, Thilo; Marchetti-Deschmann, Martina; Allmaier, Günter; Marko-Varga, György LU and Andersson, Roland LU (2016) In International Journal of Pharmaceutics 513(1-2). p.309-318
Abstract

For drug delivery, characterization of liposomes regarding size, particle number concentrations, occurrence of low-sized liposome artefacts and drug encapsulation are of importance to understand their pharmacodynamic properties. In our study, we aimed to demonstrate the applicability of nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analyser (nES GEMMA) as a suitable technique for analyzing these parameters. We measured number-based particle concentrations, identified differences in size between nominally identical liposomal samples, and detected the presence of low-diameter material which yielded bimodal particle size distributions. Subsequently, we compared these findings to dynamic light scattering (DLS) data and... (More)

For drug delivery, characterization of liposomes regarding size, particle number concentrations, occurrence of low-sized liposome artefacts and drug encapsulation are of importance to understand their pharmacodynamic properties. In our study, we aimed to demonstrate the applicability of nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analyser (nES GEMMA) as a suitable technique for analyzing these parameters. We measured number-based particle concentrations, identified differences in size between nominally identical liposomal samples, and detected the presence of low-diameter material which yielded bimodal particle size distributions. Subsequently, we compared these findings to dynamic light scattering (DLS) data and results from light scattering experiments coupled to Asymmetric Flow-Field Flow Fractionation (AF4), the latter improving the detectability of smaller particles in polydisperse samples due to a size separation step prior detection. However, the bimodal size distribution could not be detected due to method inherent limitations. In contrast, cryo transmission electron microscopy corroborated nES GEMMA results. Hence, gas-phase electrophoresis proved to be a versatile tool for liposome characterization as it could analyze both vesicle size and size distribution. Finally, a correlation of nES GEMMA results with cell viability experiments was carried out to demonstrate the importance of liposome batch-to-batch control as low-sized sample components possibly impact cell viability.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cytotoxicity, Dynamic light scattering, Flow field flow fractionation, Gas-phase electrophoretic mobility molecular analysis, Liposome, Transmission electron microscopy
in
International Journal of Pharmaceutics
volume
513
issue
1-2
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:84988023750
  • wos:000386779700033
ISSN
0378-5173
DOI
10.1016/j.ijpharm.2016.09.049
language
English
LU publication?
yes
id
2fe0a935-3706-416c-a615-db312f6df395
date added to LUP
2016-10-12 14:20:32
date last changed
2017-01-01 08:36:31
@article{2fe0a935-3706-416c-a615-db312f6df395,
  abstract     = {<p>For drug delivery, characterization of liposomes regarding size, particle number concentrations, occurrence of low-sized liposome artefacts and drug encapsulation are of importance to understand their pharmacodynamic properties. In our study, we aimed to demonstrate the applicability of nano Electrospray Gas-Phase Electrophoretic Mobility Molecular Analyser (nES GEMMA) as a suitable technique for analyzing these parameters. We measured number-based particle concentrations, identified differences in size between nominally identical liposomal samples, and detected the presence of low-diameter material which yielded bimodal particle size distributions. Subsequently, we compared these findings to dynamic light scattering (DLS) data and results from light scattering experiments coupled to Asymmetric Flow-Field Flow Fractionation (AF4), the latter improving the detectability of smaller particles in polydisperse samples due to a size separation step prior detection. However, the bimodal size distribution could not be detected due to method inherent limitations. In contrast, cryo transmission electron microscopy corroborated nES GEMMA results. Hence, gas-phase electrophoresis proved to be a versatile tool for liposome characterization as it could analyze both vesicle size and size distribution. Finally, a correlation of nES GEMMA results with cell viability experiments was carried out to demonstrate the importance of liposome batch-to-batch control as low-sized sample components possibly impact cell viability.</p>},
  author       = {Urey, Carlos and Weiss, Victor U. and Gondikas, Andreas and von der Kammer, Frank and Hofmann, Thilo and Marchetti-Deschmann, Martina and Allmaier, Günter and Marko-Varga, György and Andersson, Roland},
  issn         = {0378-5173},
  keyword      = {Cytotoxicity,Dynamic light scattering,Flow field flow fractionation,Gas-phase electrophoretic mobility molecular analysis,Liposome,Transmission electron microscopy},
  language     = {eng},
  month        = {11},
  number       = {1-2},
  pages        = {309--318},
  publisher    = {Elsevier},
  series       = {International Journal of Pharmaceutics},
  title        = {Combining gas-phase electrophoretic mobility molecular analysis (GEMMA), light scattering, field flow fractionation and cryo electron microscopy in a multidimensional approach to characterize liposomal carrier vesicles},
  url          = {http://dx.doi.org/10.1016/j.ijpharm.2016.09.049},
  volume       = {513},
  year         = {2016},
}