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NMR quantification of diffusional exchange in cell suspensions with relaxation rate differences between intra and extracellular compartments

Eriksson, Stefanie LU ; Elbing, Karin LU ; Söderman, Olle LU ; Lindkvist-Petersson, Karin LU ; Topgaard, Daniel LU and Lasič, Samo (2017) In PLoS ONE 12(5).
Abstract

Water transport across cell membranes can be measured non-invasively with diffusion NMR. We present a method to quantify the intracellular lifetime of water in cell suspensions with short transverse relaxation times, T2, and also circumvent the confounding effect of different T2 values in the intra- and extracellular compartments. Filter exchange spectroscopy (FEXSY) is specifically sensitive to exchange between compartments with different apparent diffusivities. Our investigation shows that FEXSY could yield significantly biased results if differences in T2 are not accounted for. To mitigate this problem, we propose combining FEXSY with diffusion-relaxation correlation experiment, which can quantify... (More)

Water transport across cell membranes can be measured non-invasively with diffusion NMR. We present a method to quantify the intracellular lifetime of water in cell suspensions with short transverse relaxation times, T2, and also circumvent the confounding effect of different T2 values in the intra- and extracellular compartments. Filter exchange spectroscopy (FEXSY) is specifically sensitive to exchange between compartments with different apparent diffusivities. Our investigation shows that FEXSY could yield significantly biased results if differences in T2 are not accounted for. To mitigate this problem, we propose combining FEXSY with diffusion-relaxation correlation experiment, which can quantify differences in T2 values in compartments with different diffusivities. Our analysis uses a joint constrained fitting of the two datasets and considers the effects of diffusion, relaxation and exchange in both experiments. The method is demonstrated on yeast cells with and without human aquaporins.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
PLoS ONE
volume
12
issue
5
article number
e0177273
publisher
Public Library of Science (PLoS)
external identifiers
  • pmid:28493928
  • wos:000401314300080
  • scopus:85019064219
ISSN
1932-6203
DOI
10.1371/journal.pone.0177273
language
English
LU publication?
yes
id
3000dbd0-aeb0-49b2-bd8a-3f4ec3ad5fa6
date added to LUP
2017-06-08 16:29:10
date last changed
2025-01-07 14:57:12
@article{3000dbd0-aeb0-49b2-bd8a-3f4ec3ad5fa6,
  abstract     = {{<p>Water transport across cell membranes can be measured non-invasively with diffusion NMR. We present a method to quantify the intracellular lifetime of water in cell suspensions with short transverse relaxation times, T<sub>2</sub>, and also circumvent the confounding effect of different T<sub>2</sub> values in the intra- and extracellular compartments. Filter exchange spectroscopy (FEXSY) is specifically sensitive to exchange between compartments with different apparent diffusivities. Our investigation shows that FEXSY could yield significantly biased results if differences in T<sub>2</sub> are not accounted for. To mitigate this problem, we propose combining FEXSY with diffusion-relaxation correlation experiment, which can quantify differences in T<sub>2</sub> values in compartments with different diffusivities. Our analysis uses a joint constrained fitting of the two datasets and considers the effects of diffusion, relaxation and exchange in both experiments. The method is demonstrated on yeast cells with and without human aquaporins.</p>}},
  author       = {{Eriksson, Stefanie and Elbing, Karin and Söderman, Olle and Lindkvist-Petersson, Karin and Topgaard, Daniel and Lasič, Samo}},
  issn         = {{1932-6203}},
  language     = {{eng}},
  month        = {{05}},
  number       = {{5}},
  publisher    = {{Public Library of Science (PLoS)}},
  series       = {{PLoS ONE}},
  title        = {{NMR quantification of diffusional exchange in cell suspensions with relaxation rate differences between intra and extracellular compartments}},
  url          = {{http://dx.doi.org/10.1371/journal.pone.0177273}},
  doi          = {{10.1371/journal.pone.0177273}},
  volume       = {{12}},
  year         = {{2017}},
}