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Hormonemate, a new cytotoxic and apoptosis-inducing compound from the endophytic fungus Hormonema dematioides. I. Identification of the producing strain, and isolation and biological properties of hormonemate

Filip, P; Weber, RWS; Sterner, Olov LU and Anke, T (2003) In Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences 58(7-8). p.547-552
Abstract
In a search for new fungal compounds inducing apoptosis of the colon cancer derived cell line COLO-320, hormonemate (1) was purified from fermentations of an endophytic fungus isolated from living needles of a Pinus species. The producing strain was identified as Hormonema dematioides by microscopy and ITS rDNA sequence analysis. The structure of hormonemate was determined by spectroscopic methods. The compound exhibited cytotoxic effects against the human colon tumor cell lines COLO-320, DLD-1 and HT-29 as well as five other human cell lines (HL-60, JURKAT, HEP-G2, MCF-7, HeLa S3). It also induced apoptosis in COLO-320 cells as detected by a caspase-activity assay and morphological changes, and it triggered morphological and physiological... (More)
In a search for new fungal compounds inducing apoptosis of the colon cancer derived cell line COLO-320, hormonemate (1) was purified from fermentations of an endophytic fungus isolated from living needles of a Pinus species. The producing strain was identified as Hormonema dematioides by microscopy and ITS rDNA sequence analysis. The structure of hormonemate was determined by spectroscopic methods. The compound exhibited cytotoxic effects against the human colon tumor cell lines COLO-320, DLD-1 and HT-29 as well as five other human cell lines (HL-60, JURKAT, HEP-G2, MCF-7, HeLa S3). It also induced apoptosis in COLO-320 cells as detected by a caspase-activity assay and morphological changes, and it triggered morphological and physiological differentiation of HL-60 cells into granulocytes, which subsequently died by apoptosis. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hormonemate, endophyte, Hormonema dematioides
in
Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences
volume
58
issue
7-8
pages
547 - 552
publisher
Verlag der Zeitschrift für Naturforschung
external identifiers
  • wos:000185144500017
  • scopus:0042156720
ISSN
0939-5075
language
English
LU publication?
yes
id
1fe98788-05f0-484a-be2a-f3a64d4ab455 (old id 301542)
alternative location
http://www.znaturforsch.com/ac/v58c/c58c.htm#No7
date added to LUP
2007-09-03 08:14:32
date last changed
2018-05-29 11:23:55
@article{1fe98788-05f0-484a-be2a-f3a64d4ab455,
  abstract     = {In a search for new fungal compounds inducing apoptosis of the colon cancer derived cell line COLO-320, hormonemate (1) was purified from fermentations of an endophytic fungus isolated from living needles of a Pinus species. The producing strain was identified as Hormonema dematioides by microscopy and ITS rDNA sequence analysis. The structure of hormonemate was determined by spectroscopic methods. The compound exhibited cytotoxic effects against the human colon tumor cell lines COLO-320, DLD-1 and HT-29 as well as five other human cell lines (HL-60, JURKAT, HEP-G2, MCF-7, HeLa S3). It also induced apoptosis in COLO-320 cells as detected by a caspase-activity assay and morphological changes, and it triggered morphological and physiological differentiation of HL-60 cells into granulocytes, which subsequently died by apoptosis.},
  author       = {Filip, P and Weber, RWS and Sterner, Olov and Anke, T},
  issn         = {0939-5075},
  keyword      = {hormonemate,endophyte,Hormonema dematioides},
  language     = {eng},
  number       = {7-8},
  pages        = {547--552},
  publisher    = {Verlag der Zeitschrift für Naturforschung},
  series       = {Zeitschrift fuer Naturforschung. Section C: A Journal of Biosciences},
  title        = {Hormonemate, a new cytotoxic and apoptosis-inducing compound from the endophytic fungus Hormonema dematioides. I. Identification of the producing strain, and isolation and biological properties of hormonemate},
  volume       = {58},
  year         = {2003},
}