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Two-hundredfold volume concentration of dilute cell and particle suspensions using chip integrated multistage acoustophoresis.

Nordin, Maria LU and Laurell, Thomas LU (2012) In Lab on A Chip 12(22). p.4610-4616
Abstract
Concentrating cells is a frequently performed step in cell biological assays and medical diagnostics. The commonly used centrifuge exhibits limitations when dealing with rare cell events and small sample volumes. Here, we present an acoustophoresis microfluidic chip utilising ultrasound to concentrate particles and cells into a smaller volume. The method is label-free, continuous and independent of suspending fluid, allowing for low cost and minimal preparation of the samples. Sequential concentration regions and two-dimensional acoustic standing wave focusing of cells and particles were found critical to accomplish concentration factors beyond one hundred times. Microparticles (5 μm in diameter) used to characterize the system were... (More)
Concentrating cells is a frequently performed step in cell biological assays and medical diagnostics. The commonly used centrifuge exhibits limitations when dealing with rare cell events and small sample volumes. Here, we present an acoustophoresis microfluidic chip utilising ultrasound to concentrate particles and cells into a smaller volume. The method is label-free, continuous and independent of suspending fluid, allowing for low cost and minimal preparation of the samples. Sequential concentration regions and two-dimensional acoustic standing wave focusing of cells and particles were found critical to accomplish concentration factors beyond one hundred times. Microparticles (5 μm in diameter) used to characterize the system were concentrated up to 194.2 ± 9.6 times with a recovery of 97.1 ± 4.8%. Red blood cells and prostate cancer cells were concentrated 145.0 ± 5.0 times and 195.7 ± 36.2 times, respectively, with recoveries of 97.2 ± 3.3% and 97.9 ± 18.1%. The data demonstrate that acoustophoresis is an effective technique for continuous flow-based concentration of cells and particles, offering a much needed intermediate step between sorting and detection of rare cell samples in lab-on-a-chip systems. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
12
issue
22
pages
4610 - 4616
publisher
Royal Society of Chemistry
external identifiers
  • pmid:2291841
  • wos:000310865200004
  • scopus:84867524215
ISSN
1473-0189
DOI
10.1039/c2lc40629b
language
English
LU publication?
yes
id
d172b7de-5f0a-4cfe-99e7-323990d27cce (old id 3047247)
date added to LUP
2012-10-16 13:48:41
date last changed
2017-11-05 03:10:30
@article{d172b7de-5f0a-4cfe-99e7-323990d27cce,
  abstract     = {Concentrating cells is a frequently performed step in cell biological assays and medical diagnostics. The commonly used centrifuge exhibits limitations when dealing with rare cell events and small sample volumes. Here, we present an acoustophoresis microfluidic chip utilising ultrasound to concentrate particles and cells into a smaller volume. The method is label-free, continuous and independent of suspending fluid, allowing for low cost and minimal preparation of the samples. Sequential concentration regions and two-dimensional acoustic standing wave focusing of cells and particles were found critical to accomplish concentration factors beyond one hundred times. Microparticles (5 μm in diameter) used to characterize the system were concentrated up to 194.2 ± 9.6 times with a recovery of 97.1 ± 4.8%. Red blood cells and prostate cancer cells were concentrated 145.0 ± 5.0 times and 195.7 ± 36.2 times, respectively, with recoveries of 97.2 ± 3.3% and 97.9 ± 18.1%. The data demonstrate that acoustophoresis is an effective technique for continuous flow-based concentration of cells and particles, offering a much needed intermediate step between sorting and detection of rare cell samples in lab-on-a-chip systems.},
  author       = {Nordin, Maria and Laurell, Thomas},
  issn         = {1473-0189},
  language     = {eng},
  number       = {22},
  pages        = {4610--4616},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Two-hundredfold volume concentration of dilute cell and particle suspensions using chip integrated multistage acoustophoresis.},
  url          = {http://dx.doi.org/10.1039/c2lc40629b},
  volume       = {12},
  year         = {2012},
}