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Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis) - a cross-sectional analysis.

Swärd, Per LU ; Frobell, Richard LU ; Englund, Martin LU ; Roos, Harald LU and Struglics, André LU (2012) In Osteoarthritis and Cartilage 20(11). p.1302-1308
Abstract
PURPOSE:

To in a cross-sectional study population investigate concentrations of cartilage and bone markers, and pro-inflammatory cytokines in synovial fluid (SF) collected at different time-points from acutely injured knees with hemarthrosis and to compare these with SF concentrations of knees of age and gender-matched healthy reference subjects.



METHODS:

SF was aspirated from the acutely injured knee of 111 individuals (mean age 27 years, span 13-64 years, 22% women). Concentrations of sulfated glycosaminoglycan (sGAG) were measured by Alcian blue precipitation whereas cartilage ARGS, bone biomarkers [osteocalcin (OCL), secreted protein acidic and rich in cysteine (SPARC) and osteopontin (OPN)] and... (More)
PURPOSE:

To in a cross-sectional study population investigate concentrations of cartilage and bone markers, and pro-inflammatory cytokines in synovial fluid (SF) collected at different time-points from acutely injured knees with hemarthrosis and to compare these with SF concentrations of knees of age and gender-matched healthy reference subjects.



METHODS:

SF was aspirated from the acutely injured knee of 111 individuals (mean age 27 years, span 13-64 years, 22% women). Concentrations of sulfated glycosaminoglycan (sGAG) were measured by Alcian blue precipitation whereas cartilage ARGS, bone biomarkers [osteocalcin (OCL), secreted protein acidic and rich in cysteine (SPARC) and osteopontin (OPN)] and pro-inflammatory cytokines [interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α] were analyzed using electrochemiluminescence. Samples were also analyzed with regard to time between injury and aspiration [same day (n = 29), 1 day (n = 31), 2-3 days (n = 19), 4-7 days (n = 20) and 8-23 days (n = 12)].



RESULTS:

SF concentrations of ARGS (P < 0.001), SPARC (P < 0.001), OPN (P < 0.001), and all cytokines (P < 0.001), but not sGAG (P = 0.06) or OCL (P = 0.992), were significantly higher in injured knees compared to knees of reference subjects. The cartilage markers sGAG and ARGS were significantly higher in knees aspirated later than 1 day after injury, whereas concentrations of SPARC and OPN and all cytokines were higher in knees aspirated the same day as the injury and at all time-points thereafter.



CONCLUSIONS:

Our results suggest that an acute knee injury is associated with an instant local biochemical response to the trauma, which may affect cartilage and bone as well as the inflammatory activity. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Osteoarthritis and Cartilage
volume
20
issue
11
pages
1302 - 1308
publisher
Elsevier
external identifiers
  • wos:000309853400012
  • pmid:22874525
  • scopus:84866459107
ISSN
1063-4584
DOI
10.1016/j.joca.2012.07.021
language
English
LU publication?
yes
id
64430b61-246f-48da-900f-2c863dc6f15f (old id 3047701)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22874525?dopt=Abstract
date added to LUP
2012-09-04 21:49:24
date last changed
2017-10-01 04:57:04
@article{64430b61-246f-48da-900f-2c863dc6f15f,
  abstract     = {PURPOSE: <br/><br>
To in a cross-sectional study population investigate concentrations of cartilage and bone markers, and pro-inflammatory cytokines in synovial fluid (SF) collected at different time-points from acutely injured knees with hemarthrosis and to compare these with SF concentrations of knees of age and gender-matched healthy reference subjects. <br/><br>
<br/><br>
METHODS: <br/><br>
SF was aspirated from the acutely injured knee of 111 individuals (mean age 27 years, span 13-64 years, 22% women). Concentrations of sulfated glycosaminoglycan (sGAG) were measured by Alcian blue precipitation whereas cartilage ARGS, bone biomarkers [osteocalcin (OCL), secreted protein acidic and rich in cysteine (SPARC) and osteopontin (OPN)] and pro-inflammatory cytokines [interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α] were analyzed using electrochemiluminescence. Samples were also analyzed with regard to time between injury and aspiration [same day (n = 29), 1 day (n = 31), 2-3 days (n = 19), 4-7 days (n = 20) and 8-23 days (n = 12)]. <br/><br>
<br/><br>
RESULTS: <br/><br>
SF concentrations of ARGS (P &lt; 0.001), SPARC (P &lt; 0.001), OPN (P &lt; 0.001), and all cytokines (P &lt; 0.001), but not sGAG (P = 0.06) or OCL (P = 0.992), were significantly higher in injured knees compared to knees of reference subjects. The cartilage markers sGAG and ARGS were significantly higher in knees aspirated later than 1 day after injury, whereas concentrations of SPARC and OPN and all cytokines were higher in knees aspirated the same day as the injury and at all time-points thereafter. <br/><br>
<br/><br>
CONCLUSIONS: <br/><br>
Our results suggest that an acute knee injury is associated with an instant local biochemical response to the trauma, which may affect cartilage and bone as well as the inflammatory activity.},
  author       = {Swärd, Per and Frobell, Richard and Englund, Martin and Roos, Harald and Struglics, André},
  issn         = {1063-4584},
  language     = {eng},
  number       = {11},
  pages        = {1302--1308},
  publisher    = {Elsevier},
  series       = {Osteoarthritis and Cartilage},
  title        = {Cartilage and bone markers and inflammatory cytokines are increased in synovial fluid in the acute phase of knee injury (hemarthrosis) - a cross-sectional analysis.},
  url          = {http://dx.doi.org/10.1016/j.joca.2012.07.021},
  volume       = {20},
  year         = {2012},
}