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Cytogenetic analyses of secondary liver tumors reveal significant differences in genomic imbalances between primary and metastatic colon carcinomas

Parada, Luis Antonio; Maranon, Antonio LU ; Hallén, Magnus LU ; Tranberg, Karl-Göran LU ; Stenram, Unne LU ; Bardi, Georgia and Johansson, Bertil LU (1999) In Clinical and Experimental Metastasis 17(6). p.9-471
Abstract
To investigate if karyotypic features of secondary liver tumors may provide diagnostic information and if the cytogenetic patterns of primary and metastatic colorectal carcinomas (CRC) are different, 33 liver metastases were analyzed: 25 CRC, 4 small intestine carcinoids, 1 ovarian carcinoid, 1 lobular breast cancer, 1 head-and-neck squamous cell carcinoma, and 1 uveal malignant melanoma. Chromosomal aberrations were detected in 24 cases, whereas 5 had normal karyotypes and 4 were uninformative due to lack of mitoses. Trisomy 12 was detected in 2 small intestine carcinoids, suggesting that +12 may be of pathogenetic importance in this tumor type. The breast and head-and-neck carcinomas and the uveal melanoma displayed aberrations... (More)
To investigate if karyotypic features of secondary liver tumors may provide diagnostic information and if the cytogenetic patterns of primary and metastatic colorectal carcinomas (CRC) are different, 33 liver metastases were analyzed: 25 CRC, 4 small intestine carcinoids, 1 ovarian carcinoid, 1 lobular breast cancer, 1 head-and-neck squamous cell carcinoma, and 1 uveal malignant melanoma. Chromosomal aberrations were detected in 24 cases, whereas 5 had normal karyotypes and 4 were uninformative due to lack of mitoses. Trisomy 12 was detected in 2 small intestine carcinoids, suggesting that +12 may be of pathogenetic importance in this tumor type. The breast and head-and-neck carcinomas and the uveal melanoma displayed aberrations previously reported as characteristic in primary tumors, e.g., der(1;16) and deletion of 3p in the breast cancer, losses of 3p and 8p and partial gain of 8q in the head-and-neck carcinoma, and monosomy 3 and i(8)(q10) in the uveal melanoma, indicating that cytogenetic investigations provide important diagnostic information in secondary liver tumors. In the 18 CRC metastases with chromosomal abnormalities, the cytogenetic findings agreed well with previously reported primary CRC. Common numerical abnormalities included gains of chromosomes 7, 11, 13, and 20, and losses of Y, 4, 18, 21, and 22. Structural rearrangements most often affected chromosome bands 1p13, 1q10, 3p21, 5q10, 5q11, 7q10, 8q10, 8q11, 12q13, 16p13, 17p11, 20p13, 20p11, and 20q10, and frequently resulted in losses of 1p, 8p, and 17p, and gains of 5p, 6p, 7p, 8q, and 20q. Comparing the present cases with primary CRC previously analyzed in our department revealed that additional gains of 6p, 6q, 7p, and 20q, and losses of 1p, 4p, 4q, 8p, 18p, 18q, and 22 were more common (P < 0.05) in the metastases, suggesting that these genomic sites harbor genes of importance in the metastatic process of CRC. (Less)
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Contribution to journal
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published
subject
keywords
Genome, Female, Colonic Neoplasms/ genetics/pathology, Chromosome Disorders, Chromosome Aberrations, 80 and over, Adult, Aged, Humans, Karyotyping, Liver Neoplasms/ genetics/secondary, Male, Middle Aged
in
Clinical and Experimental Metastasis
volume
17
issue
6
pages
9 - 471
publisher
Springer
external identifiers
  • scopus:0033504868
ISSN
1573-7276
language
English
LU publication?
yes
id
766c44ca-75c1-42df-a967-6c5ea8c4e47b (old id 3052309)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/10763912
http://link.springer.com/article/10.1023%2FA%3A1006646901556
date added to LUP
2013-05-07 14:21:38
date last changed
2017-11-19 04:18:53
@article{766c44ca-75c1-42df-a967-6c5ea8c4e47b,
  abstract     = {To investigate if karyotypic features of secondary liver tumors may provide diagnostic information and if the cytogenetic patterns of primary and metastatic colorectal carcinomas (CRC) are different, 33 liver metastases were analyzed: 25 CRC, 4 small intestine carcinoids, 1 ovarian carcinoid, 1 lobular breast cancer, 1 head-and-neck squamous cell carcinoma, and 1 uveal malignant melanoma. Chromosomal aberrations were detected in 24 cases, whereas 5 had normal karyotypes and 4 were uninformative due to lack of mitoses. Trisomy 12 was detected in 2 small intestine carcinoids, suggesting that +12 may be of pathogenetic importance in this tumor type. The breast and head-and-neck carcinomas and the uveal melanoma displayed aberrations previously reported as characteristic in primary tumors, e.g., der(1;16) and deletion of 3p in the breast cancer, losses of 3p and 8p and partial gain of 8q in the head-and-neck carcinoma, and monosomy 3 and i(8)(q10) in the uveal melanoma, indicating that cytogenetic investigations provide important diagnostic information in secondary liver tumors. In the 18 CRC metastases with chromosomal abnormalities, the cytogenetic findings agreed well with previously reported primary CRC. Common numerical abnormalities included gains of chromosomes 7, 11, 13, and 20, and losses of Y, 4, 18, 21, and 22. Structural rearrangements most often affected chromosome bands 1p13, 1q10, 3p21, 5q10, 5q11, 7q10, 8q10, 8q11, 12q13, 16p13, 17p11, 20p13, 20p11, and 20q10, and frequently resulted in losses of 1p, 8p, and 17p, and gains of 5p, 6p, 7p, 8q, and 20q. Comparing the present cases with primary CRC previously analyzed in our department revealed that additional gains of 6p, 6q, 7p, and 20q, and losses of 1p, 4p, 4q, 8p, 18p, 18q, and 22 were more common (P &lt; 0.05) in the metastases, suggesting that these genomic sites harbor genes of importance in the metastatic process of CRC.},
  author       = {Parada, Luis Antonio and Maranon, Antonio and Hallén, Magnus and Tranberg, Karl-Göran and Stenram, Unne and Bardi, Georgia and Johansson, Bertil},
  issn         = {1573-7276},
  keyword      = {Genome,Female,Colonic Neoplasms/ genetics/pathology,Chromosome Disorders,Chromosome Aberrations,80 and over,Adult,Aged,Humans,Karyotyping,Liver Neoplasms/ genetics/secondary,Male,Middle Aged},
  language     = {eng},
  number       = {6},
  pages        = {9--471},
  publisher    = {Springer},
  series       = {Clinical and Experimental Metastasis},
  title        = {Cytogenetic analyses of secondary liver tumors reveal significant differences in genomic imbalances between primary and metastatic colon carcinomas},
  volume       = {17},
  year         = {1999},
}