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Comparison of spatially and temporally resolved diffuse-reflectance measurement systems for determination of biomedical optical properties

Swartling, Johannes LU ; Dam, JS and Andersson-Engels, Stefan LU (2003) In Applied Optics 42(22). p.4612-4620
Abstract
Time-resolved and spatially resolved measurements of the diffuse reflectance from biological tissue are two well-established techniques for extracting the reduced scattering and absorption coefficients. We have performed a comparison study of the performance of a spatially resolved and a time-resolved instrument at wavelengths 660 and 785 nm and also of an integrating-sphere setup at 550-800 nm. The first system records the diffuse reflectance from a diode laser by means of a fiber bundle probe in contact with the sample. The time-resolved system utilizes picosecond laser pulses and a single-photon-counting detection scheme. We extracted the optical properties by calibration using known standards for the spatially resolved system, by... (More)
Time-resolved and spatially resolved measurements of the diffuse reflectance from biological tissue are two well-established techniques for extracting the reduced scattering and absorption coefficients. We have performed a comparison study of the performance of a spatially resolved and a time-resolved instrument at wavelengths 660 and 785 nm and also of an integrating-sphere setup at 550-800 nm. The first system records the diffuse reflectance from a diode laser by means of a fiber bundle probe in contact with the sample. The time-resolved system utilizes picosecond laser pulses and a single-photon-counting detection scheme. We extracted the optical properties by calibration using known standards for the spatially resolved system, by fitting to the diffusion equation for the time-resolved system, and by using an inverse Monte Carlo model for the integrating sphere. The measurements were performed on a set of solid epoxy tissue phantoms. The results showed less than 10% difference in the evaluation of the reduced scattering coefficient among the systems for the phantoms in the range 9-20 cm(-1), and absolute differences of less than 0.05 cm(-1) for the absorption coefficient in the interval 0.05-0.30 cm(-1). (C) 2003 Optical Society of America. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied Optics
volume
42
issue
22
pages
4612 - 4620
publisher
OSA
external identifiers
  • pmid:12916630
  • wos:000184382000028
  • scopus:0041880625
ISSN
2155-3165
language
English
LU publication?
yes
id
bde47686-1574-40fa-8fb5-2ba7b35ca280 (old id 305790)
alternative location
http://www.opticsinfobase.org/abstract.cfm?URI=ao-42-22-4612
date added to LUP
2007-09-23 09:47:49
date last changed
2018-01-07 05:30:00
@article{bde47686-1574-40fa-8fb5-2ba7b35ca280,
  abstract     = {Time-resolved and spatially resolved measurements of the diffuse reflectance from biological tissue are two well-established techniques for extracting the reduced scattering and absorption coefficients. We have performed a comparison study of the performance of a spatially resolved and a time-resolved instrument at wavelengths 660 and 785 nm and also of an integrating-sphere setup at 550-800 nm. The first system records the diffuse reflectance from a diode laser by means of a fiber bundle probe in contact with the sample. The time-resolved system utilizes picosecond laser pulses and a single-photon-counting detection scheme. We extracted the optical properties by calibration using known standards for the spatially resolved system, by fitting to the diffusion equation for the time-resolved system, and by using an inverse Monte Carlo model for the integrating sphere. The measurements were performed on a set of solid epoxy tissue phantoms. The results showed less than 10% difference in the evaluation of the reduced scattering coefficient among the systems for the phantoms in the range 9-20 cm(-1), and absolute differences of less than 0.05 cm(-1) for the absorption coefficient in the interval 0.05-0.30 cm(-1). (C) 2003 Optical Society of America.},
  author       = {Swartling, Johannes and Dam, JS and Andersson-Engels, Stefan},
  issn         = {2155-3165},
  language     = {eng},
  number       = {22},
  pages        = {4612--4620},
  publisher    = {OSA},
  series       = {Applied Optics},
  title        = {Comparison of spatially and temporally resolved diffuse-reflectance measurement systems for determination of biomedical optical properties},
  volume       = {42},
  year         = {2003},
}