Outer membrane vesicles shield Moraxella catarrhalis β-lactamase from neutralization by serum IgG.
Schaar, Viveka LU ; Paulsson, Magnus LU
; Mörgelin, Matthias
LU
and Riesbeck, Kristian
LU
(2013)
In Journal of Antimicrobial Chemotherapy
68(3).
p.593-600
- Abstract
- OBJECTIVES: The aim of this study was to detect the presence of IgG against Moraxella catarrhalis β-lactamase in healthy adults, and to determine whether outer membrane vesicles (OMVs) could protect the enzyme from inhibition by anti-β-lactamase IgG. METHODS: Transmission electron microscopy was used to detect the presence of β-lactamase in OMVs. Sera were examined by ELISA for specific IgG directed against recombinant M. catarrhalis β-lactamase in addition to the outer membrane adhesins MID/Hag, UspA1 and A2. Binding of anti-β-lactamase IgG from serum to OMVs was analysed by flow cytometry. The chromogenic substrate nitrocefin was used to quantify β-lactamase enzyme activity. RESULTS: The presence of β-lactamase was determined in OMVs... (More)
- OBJECTIVES: The aim of this study was to detect the presence of IgG against Moraxella catarrhalis β-lactamase in healthy adults, and to determine whether outer membrane vesicles (OMVs) could protect the enzyme from inhibition by anti-β-lactamase IgG. METHODS: Transmission electron microscopy was used to detect the presence of β-lactamase in OMVs. Sera were examined by ELISA for specific IgG directed against recombinant M. catarrhalis β-lactamase in addition to the outer membrane adhesins MID/Hag, UspA1 and A2. Binding of anti-β-lactamase IgG from serum to OMVs was analysed by flow cytometry. The chromogenic substrate nitrocefin was used to quantify β-lactamase enzyme activity. RESULTS: The presence of β-lactamase was determined in OMVs from a 9-year-old child suffering from M. catarrhalis sinusitis. Furthermore, anti-β-lactamase IgG was detected in sera obtained from healthy adults. Out of 40 adult blood donors (aged 18-65 years) tested, 6 (15.0%) carried anti-β-lactamase IgG. No correlation between IgG titres against β-lactamase and the adhesins was found. Flow cytometry analyses revealed that anti-β-lactamase IgG from serum bound to β-lactamase-positive OMVs. By comparing the β-lactamase activity of intact OMV with OMV that were permeabilized with saponin we found that OMVs shielded active β-lactamase from the anti-β-lactamase IgG. CONCLUSIONS: Moraxella catarrhalis β-lactamase is found in, or associated with, OMVs, providing clinical relevance for the vesicles in the spread of antibiotic resistance. Furthermore, OMVs protect β-lactamase from specific IgG. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3218462
- author
- Schaar, Viveka
LU
; Paulsson, Magnus
LU
; Mörgelin, Matthias
LU
and Riesbeck, Kristian
LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Antimicrobial Chemotherapy
- volume
- 68
- issue
- 3
- pages
- 593 - 600
- publisher
- Oxford University Press
- external identifiers
-
- wos:000314893700020
- pmid:23184710
- scopus:84873597322
- pmid:23184710
- ISSN
- 1460-2091
- DOI
- 10.1093/jac/dks444
- language
- English
- LU publication?
- yes
- id
- 3066fe04-97b3-4398-8f17-2872ea5b0a07 (old id 3218462)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23184710?dopt=Abstract
- date added to LUP
- 2016-04-01 10:47:22
- date last changed
- 2024-01-07 01:15:54
@article{3066fe04-97b3-4398-8f17-2872ea5b0a07,
abstract = {{OBJECTIVES: The aim of this study was to detect the presence of IgG against Moraxella catarrhalis β-lactamase in healthy adults, and to determine whether outer membrane vesicles (OMVs) could protect the enzyme from inhibition by anti-β-lactamase IgG. METHODS: Transmission electron microscopy was used to detect the presence of β-lactamase in OMVs. Sera were examined by ELISA for specific IgG directed against recombinant M. catarrhalis β-lactamase in addition to the outer membrane adhesins MID/Hag, UspA1 and A2. Binding of anti-β-lactamase IgG from serum to OMVs was analysed by flow cytometry. The chromogenic substrate nitrocefin was used to quantify β-lactamase enzyme activity. RESULTS: The presence of β-lactamase was determined in OMVs from a 9-year-old child suffering from M. catarrhalis sinusitis. Furthermore, anti-β-lactamase IgG was detected in sera obtained from healthy adults. Out of 40 adult blood donors (aged 18-65 years) tested, 6 (15.0%) carried anti-β-lactamase IgG. No correlation between IgG titres against β-lactamase and the adhesins was found. Flow cytometry analyses revealed that anti-β-lactamase IgG from serum bound to β-lactamase-positive OMVs. By comparing the β-lactamase activity of intact OMV with OMV that were permeabilized with saponin we found that OMVs shielded active β-lactamase from the anti-β-lactamase IgG. CONCLUSIONS: Moraxella catarrhalis β-lactamase is found in, or associated with, OMVs, providing clinical relevance for the vesicles in the spread of antibiotic resistance. Furthermore, OMVs protect β-lactamase from specific IgG.}},
author = {{Schaar, Viveka and Paulsson, Magnus and Mörgelin, Matthias and Riesbeck, Kristian}},
issn = {{1460-2091}},
language = {{eng}},
number = {{3}},
pages = {{593--600}},
publisher = {{Oxford University Press}},
series = {{Journal of Antimicrobial Chemotherapy}},
title = {{Outer membrane vesicles shield Moraxella catarrhalis β-lactamase from neutralization by serum IgG.}},
url = {{https://lup.lub.lu.se/search/files/2137826/3878916.pdf}},
doi = {{10.1093/jac/dks444}},
volume = {{68}},
year = {{2013}},
}