Advanced

Towards a 'nano-traffic' system powered by molecular motors

Bunk, Richard LU ; Klinth, J; Rosengren, J; Nicholls, I; Tagerud, S; Omling, Pär LU ; Mansson, A and Montelius, Lars LU (2003) In Microelectronic Engineering 67-8. p.899-904
Abstract
In this work, we reconstructed in vitro the behavior of two motor proteins-myosin and actin-responsible for the mechanical action of muscle cells. By transferring this in vivo system to an artificial environment, we were able to study the interaction between the proteins in more detail, as well as investigating the central mechanism of force production. Nm-patterning by e-beam lithography (EBL) could restore parts of the in vivo protein order, essential for potential nanotechnological applications. Much work was put into establishing the necessary compatibility between the biological and nano-lithographical processes. A range of EBL-resists were tested for protein compatibility. One particular kind (MRL-6000.1XP) supported good actin... (More)
In this work, we reconstructed in vitro the behavior of two motor proteins-myosin and actin-responsible for the mechanical action of muscle cells. By transferring this in vivo system to an artificial environment, we were able to study the interaction between the proteins in more detail, as well as investigating the central mechanism of force production. Nm-patterning by e-beam lithography (EBL) could restore parts of the in vivo protein order, essential for potential nanotechnological applications. Much work was put into establishing the necessary compatibility between the biological and nano-lithographical processes. A range of EBL-resists were tested for protein compatibility. One particular kind (MRL-6000.1XP) supported good actin filament motility, while another (PMMA-950) behaved in the opposite way. Taking advantage of these findings, nm-sized lines were created in a double-layer structure of the two resists. The lines were found to act as binding sites for myosin, and as rectifying guides for the linearized motion of actin filaments. Velocities around 5 mum/s were measured. (C) 2003 Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
actin, lab-on-a-chip, motor proteins, EBL, myosin
in
Microelectronic Engineering
volume
67-8
pages
899 - 904
publisher
Elsevier
external identifiers
  • wos:000183842100125
  • scopus:0038696440
ISSN
1873-5568
DOI
10.1016/S0167-9317(03)00194-1
language
English
LU publication?
yes
id
0bb3701a-05f9-4532-adea-185373d66268 (old id 307105)
date added to LUP
2007-08-27 12:21:57
date last changed
2018-10-03 11:29:48
@article{0bb3701a-05f9-4532-adea-185373d66268,
  abstract     = {In this work, we reconstructed in vitro the behavior of two motor proteins-myosin and actin-responsible for the mechanical action of muscle cells. By transferring this in vivo system to an artificial environment, we were able to study the interaction between the proteins in more detail, as well as investigating the central mechanism of force production. Nm-patterning by e-beam lithography (EBL) could restore parts of the in vivo protein order, essential for potential nanotechnological applications. Much work was put into establishing the necessary compatibility between the biological and nano-lithographical processes. A range of EBL-resists were tested for protein compatibility. One particular kind (MRL-6000.1XP) supported good actin filament motility, while another (PMMA-950) behaved in the opposite way. Taking advantage of these findings, nm-sized lines were created in a double-layer structure of the two resists. The lines were found to act as binding sites for myosin, and as rectifying guides for the linearized motion of actin filaments. Velocities around 5 mum/s were measured. (C) 2003 Elsevier Science B.V. All rights reserved.},
  author       = {Bunk, Richard and Klinth, J and Rosengren, J and Nicholls, I and Tagerud, S and Omling, Pär and Mansson, A and Montelius, Lars},
  issn         = {1873-5568},
  keyword      = {actin,lab-on-a-chip,motor proteins,EBL,myosin},
  language     = {eng},
  pages        = {899--904},
  publisher    = {Elsevier},
  series       = {Microelectronic Engineering},
  title        = {Towards a 'nano-traffic' system powered by molecular motors},
  url          = {http://dx.doi.org/10.1016/S0167-9317(03)00194-1},
  volume       = {67-8},
  year         = {2003},
}