Advanced

Subarachnoid hemorrhage-induced upregulation of the 5-HT1B receptor in cerebral arteries in rats

Hansen-Schwartz, J; Hoel, NL; Xu, Cang-Bao LU ; Svendgaard, NA and Edvinsson, Lars LU (2003) In Journal of Neurosurgery 99(1). p.115-120
Abstract
Object. Cerebral vasospasm following subarachnoid hemorrhage (SAH) leads to reduced blood flow in the brain. Inspired by organ culture-induced changes in the receptor phenotype of cerebral arteries, the authors investigated possible changes in the 5-hydroxytryptamine (HT) receptor phenotype after experimental SAH. Methods. Experimental SAH was induced in rats by using an autologous prechiasmatic injection of arterial blood. Two days later, the middle cerebral artery (MCA), posterior communicating artery (PCoA), and basilar artery (BA) were harvested and examined functionally with the aid of a sensitive in vitro pharmacological method and molecularly by performing quantitative real-time reverse transcription-polymerase chain reaction (PCR).... (More)
Object. Cerebral vasospasm following subarachnoid hemorrhage (SAH) leads to reduced blood flow in the brain. Inspired by organ culture-induced changes in the receptor phenotype of cerebral arteries, the authors investigated possible changes in the 5-hydroxytryptamine (HT) receptor phenotype after experimental SAH. Methods. Experimental SAH was induced in rats by using an autologous prechiasmatic injection of arterial blood. Two days later, the middle cerebral artery (MCA), posterior communicating artery (PCoA), and basilar artery (BA) were harvested and examined functionally with the aid of a sensitive in vitro pharmacological method and molecularly by performing quantitative real-time reverse transcription-polymerase chain reaction (PCR). In the MCA and BA the 5-HT1B receptor was upregulated, as determined through both functional and molecular analysis. In response to selective 5-HT1 receptor agonists both the negative logarithm of the 50% effective concentration was increased (one log unit in the MCA and one half unit in the BA), as was the agonist's potency (increased by 50% in the MCA and doubled in the BA). In addition, the authors found an approximately fourfold increase in the number of copies of messenger RNA coding for the 5-HT1B receptor as determined by quantitative real-time PCR. In the PCoA no upregulation of the 5-HT1B receptor was observed. Conclusions. Changes in the receptor phenotype in favor of contractile receptors may well represent the end stage in a sequence of events leading from SAH to the actual development of cerebral vasospasm. Insight into the mechanism of upregulation may provide new targets for developing specific treatment against cerebral vasospasm. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
5-hydroxytryptamine, serotonin, subarachnoid hemorrhage, cerebral vasospasm, rat, polymerase chain reaction
in
Journal of Neurosurgery
volume
99
issue
1
pages
115 - 120
publisher
American Association of Neurosurgeons
external identifiers
  • pmid:12854752
  • wos:000183865500018
  • scopus:0038647271
ISSN
0022-3085
language
English
LU publication?
yes
id
4490a948-95b2-4528-aeec-b93fbfd83f29 (old id 307137)
alternative location
http://www.thejns-net.org/jns/issues/v99n1/abs/n0990115_fs.html
date added to LUP
2007-09-03 14:57:53
date last changed
2018-10-03 12:10:27
@article{4490a948-95b2-4528-aeec-b93fbfd83f29,
  abstract     = {Object. Cerebral vasospasm following subarachnoid hemorrhage (SAH) leads to reduced blood flow in the brain. Inspired by organ culture-induced changes in the receptor phenotype of cerebral arteries, the authors investigated possible changes in the 5-hydroxytryptamine (HT) receptor phenotype after experimental SAH. Methods. Experimental SAH was induced in rats by using an autologous prechiasmatic injection of arterial blood. Two days later, the middle cerebral artery (MCA), posterior communicating artery (PCoA), and basilar artery (BA) were harvested and examined functionally with the aid of a sensitive in vitro pharmacological method and molecularly by performing quantitative real-time reverse transcription-polymerase chain reaction (PCR). In the MCA and BA the 5-HT1B receptor was upregulated, as determined through both functional and molecular analysis. In response to selective 5-HT1 receptor agonists both the negative logarithm of the 50% effective concentration was increased (one log unit in the MCA and one half unit in the BA), as was the agonist's potency (increased by 50% in the MCA and doubled in the BA). In addition, the authors found an approximately fourfold increase in the number of copies of messenger RNA coding for the 5-HT1B receptor as determined by quantitative real-time PCR. In the PCoA no upregulation of the 5-HT1B receptor was observed. Conclusions. Changes in the receptor phenotype in favor of contractile receptors may well represent the end stage in a sequence of events leading from SAH to the actual development of cerebral vasospasm. Insight into the mechanism of upregulation may provide new targets for developing specific treatment against cerebral vasospasm.},
  author       = {Hansen-Schwartz, J and Hoel, NL and Xu, Cang-Bao and Svendgaard, NA and Edvinsson, Lars},
  issn         = {0022-3085},
  keyword      = {5-hydroxytryptamine,serotonin,subarachnoid hemorrhage,cerebral vasospasm,rat,polymerase chain reaction},
  language     = {eng},
  number       = {1},
  pages        = {115--120},
  publisher    = {American Association of Neurosurgeons},
  series       = {Journal of Neurosurgery},
  title        = {Subarachnoid hemorrhage-induced upregulation of the 5-HT1B receptor in cerebral arteries in rats},
  volume       = {99},
  year         = {2003},
}