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Differential neurochemical properties of central serotonergic transmission in Roman high- and low-avoidance rats

Giorgi, O; Piras, G; Lecca, D; Hansson, Staffan LU ; Driscoll, P and Corda, MG (2003) In Journal of Neurochemistry 86(2). p.422-431
Abstract
The selective breeding of Roman high- (RHA/Verh) and low-avoidance (RLA/Verh) rats for rapid versus poor acquisition of active avoidant behaviour has produced two behavioural phenotypes with different performances in a variety of animal models of anxiety, in which RLA/Verh rats are consistently more fearful than RHA/Verh rats. In addition, these two lines display different functional properties of brain neurotransmitters like serotonin (5-HT), known to be involved in the expression of anxiety- and depression-related behaviours. Therefore, we used brain microdialysis and [(3) H]-citalopram binding autoradiography to characterize further the neurochemical properties of 5-HTergic transmission in the two lines. No significant line-related... (More)
The selective breeding of Roman high- (RHA/Verh) and low-avoidance (RLA/Verh) rats for rapid versus poor acquisition of active avoidant behaviour has produced two behavioural phenotypes with different performances in a variety of animal models of anxiety, in which RLA/Verh rats are consistently more fearful than RHA/Verh rats. In addition, these two lines display different functional properties of brain neurotransmitters like serotonin (5-HT), known to be involved in the expression of anxiety- and depression-related behaviours. Therefore, we used brain microdialysis and [(3) H]-citalopram binding autoradiography to characterize further the neurochemical properties of 5-HTergic transmission in the two lines. No significant line-related differences were detected in the basal 5-HT output in the frontoparietal cortex (FPCx). In contrast, the increase in the cortical 5-HT output elicited by the systemic administration or the local application, via reverse dialysis, of chlorimipramine and fluoxetine was more robust in RHA/Verh than in RLA/Verh rats. Moreover, the binding signal of [(3) H]-citalopram to 5-HT re-uptake sites was more intense in the FPCx of RHA/Verh rats than in their RLA/Verh counterparts. These findings suggest that the functional tone of the 5-HTergic projection to the FPCx is stronger in the RHA/Verh line relative to the RLA/Verh line. It is proposed that RLA/Verh rats may be used as a model with heuristic value for studying the role of 5-HTergic transmission in anxiety and in the anxiolytic effects of monoamine re-uptake inhibitors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
chlorimipramine, depression, fluoxetine, Roman high-avoidance and low-avoidance rats, brain microdialysis, anxiety
in
Journal of Neurochemistry
volume
86
issue
2
pages
422 - 431
publisher
Wiley-Blackwell
external identifiers
  • wos:000183900300016
  • pmid:12871583
  • scopus:0037479822
ISSN
1471-4159
DOI
language
English
LU publication?
yes
id
1f62f8ed-9ade-4bac-83ba-c563ee0e37a0 (old id 307143)
date added to LUP
2007-09-03 10:21:02
date last changed
2018-05-29 09:23:40
@article{1f62f8ed-9ade-4bac-83ba-c563ee0e37a0,
  abstract     = {The selective breeding of Roman high- (RHA/Verh) and low-avoidance (RLA/Verh) rats for rapid versus poor acquisition of active avoidant behaviour has produced two behavioural phenotypes with different performances in a variety of animal models of anxiety, in which RLA/Verh rats are consistently more fearful than RHA/Verh rats. In addition, these two lines display different functional properties of brain neurotransmitters like serotonin (5-HT), known to be involved in the expression of anxiety- and depression-related behaviours. Therefore, we used brain microdialysis and [(3) H]-citalopram binding autoradiography to characterize further the neurochemical properties of 5-HTergic transmission in the two lines. No significant line-related differences were detected in the basal 5-HT output in the frontoparietal cortex (FPCx). In contrast, the increase in the cortical 5-HT output elicited by the systemic administration or the local application, via reverse dialysis, of chlorimipramine and fluoxetine was more robust in RHA/Verh than in RLA/Verh rats. Moreover, the binding signal of [(3) H]-citalopram to 5-HT re-uptake sites was more intense in the FPCx of RHA/Verh rats than in their RLA/Verh counterparts. These findings suggest that the functional tone of the 5-HTergic projection to the FPCx is stronger in the RHA/Verh line relative to the RLA/Verh line. It is proposed that RLA/Verh rats may be used as a model with heuristic value for studying the role of 5-HTergic transmission in anxiety and in the anxiolytic effects of monoamine re-uptake inhibitors.},
  author       = {Giorgi, O and Piras, G and Lecca, D and Hansson, Staffan and Driscoll, P and Corda, MG},
  issn         = {1471-4159},
  keyword      = {chlorimipramine,depression,fluoxetine,Roman high-avoidance and low-avoidance rats,brain microdialysis,anxiety},
  language     = {eng},
  number       = {2},
  pages        = {422--431},
  publisher    = {Wiley-Blackwell},
  series       = {Journal of Neurochemistry},
  title        = {Differential neurochemical properties of central serotonergic transmission in Roman high- and low-avoidance rats},
  url          = {http://dx.doi.org/},
  volume       = {86},
  year         = {2003},
}