Differential neurochemical properties of central serotonergic transmission in Roman high- and low-avoidance rats
(2003) In Journal of Neurochemistry 86(2). p.422-431- Abstract
- The selective breeding of Roman high- (RHA/Verh) and low-avoidance (RLA/Verh) rats for rapid versus poor acquisition of active avoidant behaviour has produced two behavioural phenotypes with different performances in a variety of animal models of anxiety, in which RLA/Verh rats are consistently more fearful than RHA/Verh rats. In addition, these two lines display different functional properties of brain neurotransmitters like serotonin (5-HT), known to be involved in the expression of anxiety- and depression-related behaviours. Therefore, we used brain microdialysis and [(3) H]-citalopram binding autoradiography to characterize further the neurochemical properties of 5-HTergic transmission in the two lines. No significant line-related... (More)
- The selective breeding of Roman high- (RHA/Verh) and low-avoidance (RLA/Verh) rats for rapid versus poor acquisition of active avoidant behaviour has produced two behavioural phenotypes with different performances in a variety of animal models of anxiety, in which RLA/Verh rats are consistently more fearful than RHA/Verh rats. In addition, these two lines display different functional properties of brain neurotransmitters like serotonin (5-HT), known to be involved in the expression of anxiety- and depression-related behaviours. Therefore, we used brain microdialysis and [(3) H]-citalopram binding autoradiography to characterize further the neurochemical properties of 5-HTergic transmission in the two lines. No significant line-related differences were detected in the basal 5-HT output in the frontoparietal cortex (FPCx). In contrast, the increase in the cortical 5-HT output elicited by the systemic administration or the local application, via reverse dialysis, of chlorimipramine and fluoxetine was more robust in RHA/Verh than in RLA/Verh rats. Moreover, the binding signal of [(3) H]-citalopram to 5-HT re-uptake sites was more intense in the FPCx of RHA/Verh rats than in their RLA/Verh counterparts. These findings suggest that the functional tone of the 5-HTergic projection to the FPCx is stronger in the RHA/Verh line relative to the RLA/Verh line. It is proposed that RLA/Verh rats may be used as a model with heuristic value for studying the role of 5-HTergic transmission in anxiety and in the anxiolytic effects of monoamine re-uptake inhibitors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/307143
- author
- Giorgi, O ; Piras, G ; Lecca, D ; Hansson, Staffan LU ; Driscoll, P and Corda, MG
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- chlorimipramine, depression, fluoxetine, Roman high-avoidance and low-avoidance rats, brain microdialysis, anxiety
- in
- Journal of Neurochemistry
- volume
- 86
- issue
- 2
- pages
- 422 - 431
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000183900300016
- pmid:12871583
- scopus:0037479822
- ISSN
- 1471-4159
- DOI
- 10.1046/j.1471-4159.2003.01845.x
- language
- English
- LU publication?
- yes
- id
- 1f62f8ed-9ade-4bac-83ba-c563ee0e37a0 (old id 307143)
- date added to LUP
- 2016-04-01 17:08:38
- date last changed
- 2022-03-07 18:48:15
@article{1f62f8ed-9ade-4bac-83ba-c563ee0e37a0,
abstract = {{The selective breeding of Roman high- (RHA/Verh) and low-avoidance (RLA/Verh) rats for rapid versus poor acquisition of active avoidant behaviour has produced two behavioural phenotypes with different performances in a variety of animal models of anxiety, in which RLA/Verh rats are consistently more fearful than RHA/Verh rats. In addition, these two lines display different functional properties of brain neurotransmitters like serotonin (5-HT), known to be involved in the expression of anxiety- and depression-related behaviours. Therefore, we used brain microdialysis and [(3) H]-citalopram binding autoradiography to characterize further the neurochemical properties of 5-HTergic transmission in the two lines. No significant line-related differences were detected in the basal 5-HT output in the frontoparietal cortex (FPCx). In contrast, the increase in the cortical 5-HT output elicited by the systemic administration or the local application, via reverse dialysis, of chlorimipramine and fluoxetine was more robust in RHA/Verh than in RLA/Verh rats. Moreover, the binding signal of [(3) H]-citalopram to 5-HT re-uptake sites was more intense in the FPCx of RHA/Verh rats than in their RLA/Verh counterparts. These findings suggest that the functional tone of the 5-HTergic projection to the FPCx is stronger in the RHA/Verh line relative to the RLA/Verh line. It is proposed that RLA/Verh rats may be used as a model with heuristic value for studying the role of 5-HTergic transmission in anxiety and in the anxiolytic effects of monoamine re-uptake inhibitors.}},
author = {{Giorgi, O and Piras, G and Lecca, D and Hansson, Staffan and Driscoll, P and Corda, MG}},
issn = {{1471-4159}},
keywords = {{chlorimipramine; depression; fluoxetine; Roman high-avoidance and low-avoidance rats; brain microdialysis; anxiety}},
language = {{eng}},
number = {{2}},
pages = {{422--431}},
publisher = {{Wiley-Blackwell}},
series = {{Journal of Neurochemistry}},
title = {{Differential neurochemical properties of central serotonergic transmission in Roman high- and low-avoidance rats}},
url = {{http://dx.doi.org/10.1046/j.1471-4159.2003.01845.x}},
doi = {{10.1046/j.1471-4159.2003.01845.x}},
volume = {{86}},
year = {{2003}},
}