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Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion

Verhaegh, Suzanne J. C.; Stol, Kim; de Vogel, Corne P.; Riesbeck, Kristian LU ; Lafontaine, Eric R.; Murphy, Timothy F.; van Belkum, Alex; Hermans, Peter W. M. and Hays, John P. (2012) In Clinical and Vaccine Immunology 19(6). p.914-918
Abstract
A prospective clinical cohort study was established to investigate the humoral immune response in middle ear fluids (MEF) and serum against bacterial surface proteins in children suffering from recurrent acute otitis media (rAOM) and chronic otitis media with effusion (COME), using Luminex xMAP technology. The association between the humoral immune response and the presence of Moraxella catarrhalis and Streptococcus pneumoniae in the nasopharynx and middle ear was also studied. The levels of antigen-specific IgG, IgA, and IgM showed extensive interindividual variation. No significant differences in anti-M. catarrhalis and anti-S. pneumoniae serum and MEF median fluorescence intensity (MFI) values (anti-M. catarrhalis and antipneumococcal... (More)
A prospective clinical cohort study was established to investigate the humoral immune response in middle ear fluids (MEF) and serum against bacterial surface proteins in children suffering from recurrent acute otitis media (rAOM) and chronic otitis media with effusion (COME), using Luminex xMAP technology. The association between the humoral immune response and the presence of Moraxella catarrhalis and Streptococcus pneumoniae in the nasopharynx and middle ear was also studied. The levels of antigen-specific IgG, IgA, and IgM showed extensive interindividual variation. No significant differences in anti-M. catarrhalis and anti-S. pneumoniae serum and MEF median fluorescence intensity (MFI) values (anti-M. catarrhalis and antipneumococcal IgG levels) were observed between the rAOM or COME groups for all antigens tested. No significant differences were observed for M. catarrhalis and S. pneumoniae colonization and serum IgG levels against the Moraxella and pneumococcal antigens. Similar to the antibody response in serum, no significant differences in IgG, IgA, and IgM levels in MEF were observed for all M. catarrhalis and S. pneumoniae antigens between OM M. catarrhalis-or S. pneumoniae-positive and OM M. catarrhalis-or S. pneumonia-negative children suffering from either rAOM or COME. Finally, results indicated a strong correlation between antigen-specific serum and MEF IgG levels. We observed no significant in vivo expressed anti-M. catarrhalis or anti-S. pneumoniae humoral immune responses using a range of putative vaccine candidate proteins. Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Clinical and Vaccine Immunology
volume
19
issue
6
pages
914 - 918
publisher
American Society for Microbiology
external identifiers
  • wos:000307110500013
  • scopus:84861723617
ISSN
1556-6811
DOI
10.1128/CVI.05630-11
language
English
LU publication?
yes
id
aab6d1ba-ff78-44ed-9500-88461f361522 (old id 3079706)
date added to LUP
2012-10-05 07:16:12
date last changed
2017-07-23 03:29:54
@article{aab6d1ba-ff78-44ed-9500-88461f361522,
  abstract     = {A prospective clinical cohort study was established to investigate the humoral immune response in middle ear fluids (MEF) and serum against bacterial surface proteins in children suffering from recurrent acute otitis media (rAOM) and chronic otitis media with effusion (COME), using Luminex xMAP technology. The association between the humoral immune response and the presence of Moraxella catarrhalis and Streptococcus pneumoniae in the nasopharynx and middle ear was also studied. The levels of antigen-specific IgG, IgA, and IgM showed extensive interindividual variation. No significant differences in anti-M. catarrhalis and anti-S. pneumoniae serum and MEF median fluorescence intensity (MFI) values (anti-M. catarrhalis and antipneumococcal IgG levels) were observed between the rAOM or COME groups for all antigens tested. No significant differences were observed for M. catarrhalis and S. pneumoniae colonization and serum IgG levels against the Moraxella and pneumococcal antigens. Similar to the antibody response in serum, no significant differences in IgG, IgA, and IgM levels in MEF were observed for all M. catarrhalis and S. pneumoniae antigens between OM M. catarrhalis-or S. pneumoniae-positive and OM M. catarrhalis-or S. pneumonia-negative children suffering from either rAOM or COME. Finally, results indicated a strong correlation between antigen-specific serum and MEF IgG levels. We observed no significant in vivo expressed anti-M. catarrhalis or anti-S. pneumoniae humoral immune responses using a range of putative vaccine candidate proteins. Other factors, such as Eustachian tube dysfunction, viral load, and genetic and environmental factors, may play a more important role in the pathogenesis of OM and in particular in the development of rAOM or COME.},
  author       = {Verhaegh, Suzanne J. C. and Stol, Kim and de Vogel, Corne P. and Riesbeck, Kristian and Lafontaine, Eric R. and Murphy, Timothy F. and van Belkum, Alex and Hermans, Peter W. M. and Hays, John P.},
  issn         = {1556-6811},
  language     = {eng},
  number       = {6},
  pages        = {914--918},
  publisher    = {American Society for Microbiology},
  series       = {Clinical and Vaccine Immunology},
  title        = {Comparative Analysis of the Humoral Immune Response to Moraxella catarrhalis and Streptococcus pneumoniae Surface Antigens in Children Suffering from Recurrent Acute Otitis Media and Chronic Otitis Media with Effusion},
  url          = {http://dx.doi.org/10.1128/CVI.05630-11},
  volume       = {19},
  year         = {2012},
}