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Nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA) of liposomes : Applicability of the technique for nano vesicle batch control

Weiss, Victor U.; Urey, Carlos LU ; Gondikas, Andreas; Golesne, Monika; Friedbacher, Gernot; Von Der Kammer, Frank; Hofmann, Thilo; Andersson, Roland LU ; Marko-Varga, György LU and Marchetti-Deschmann, Martina, et al. (2016) In Analyst 141(21). p.6042-6050
Abstract

Liposomes are biodegradable nanoparticle vesicles consisting of a lipid bilayer encapsulating an aqueous core. Entrapped cargo material is shielded from the extra-vesicular medium and sustained release of encapsulated material can be achieved. However, application of liposomes as nano-carriers demands their characterization concerning size and size distribution, particle-number concentration, occurrence of vesicle building blocks in solution and determination of the resulting vesicle encapsulation capacity. These questions can be targeted via gas-phase electrophoretic mobility molecular analysis (GEMMA) based on a nano electrospray (nES) charge-reduction source. This instrument separates single-charged nanoparticles in the gas-phase... (More)

Liposomes are biodegradable nanoparticle vesicles consisting of a lipid bilayer encapsulating an aqueous core. Entrapped cargo material is shielded from the extra-vesicular medium and sustained release of encapsulated material can be achieved. However, application of liposomes as nano-carriers demands their characterization concerning size and size distribution, particle-number concentration, occurrence of vesicle building blocks in solution and determination of the resulting vesicle encapsulation capacity. These questions can be targeted via gas-phase electrophoretic mobility molecular analysis (GEMMA) based on a nano electrospray (nES) charge-reduction source. This instrument separates single-charged nanoparticles in the gas-phase according to size in a high-laminar sheath-flow by means of an orthogonal, tunable electric field. nES GEMMA analysis enables to confirm liposome integrity after passage through the instrument (in combination with atomic force microscopy) as well as to exclude vesicle aggregation. Additionally, nanoparticle diameters at peak apexes and size distribution data are obtained. Differences of hydrodynamic and dry particle diameter values, as well as the effect of number- and mass-based concentration data analysis on obtained liposome diameters are shown. Furthermore, the repeatability of liposome preparation is studied, especially upon incorporation of PEGylated lipids in the bilayer. Finally, the instruments applicability to monitor mechanical stress applied to vesicles is demonstrated.

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publication status
published
subject
in
Analyst
volume
141
issue
21
pages
9 pages
publisher
Royal Society of Chemistry
external identifiers
  • scopus:84988023035
  • wos:000386210900012
ISSN
0003-2654
DOI
10.1039/c6an00687f
language
English
LU publication?
yes
id
307bf76f-9cc2-4981-91b5-d842cee29d62
date added to LUP
2016-11-04 10:40:40
date last changed
2017-02-26 04:42:26
@article{307bf76f-9cc2-4981-91b5-d842cee29d62,
  abstract     = {<p>Liposomes are biodegradable nanoparticle vesicles consisting of a lipid bilayer encapsulating an aqueous core. Entrapped cargo material is shielded from the extra-vesicular medium and sustained release of encapsulated material can be achieved. However, application of liposomes as nano-carriers demands their characterization concerning size and size distribution, particle-number concentration, occurrence of vesicle building blocks in solution and determination of the resulting vesicle encapsulation capacity. These questions can be targeted via gas-phase electrophoretic mobility molecular analysis (GEMMA) based on a nano electrospray (nES) charge-reduction source. This instrument separates single-charged nanoparticles in the gas-phase according to size in a high-laminar sheath-flow by means of an orthogonal, tunable electric field. nES GEMMA analysis enables to confirm liposome integrity after passage through the instrument (in combination with atomic force microscopy) as well as to exclude vesicle aggregation. Additionally, nanoparticle diameters at peak apexes and size distribution data are obtained. Differences of hydrodynamic and dry particle diameter values, as well as the effect of number- and mass-based concentration data analysis on obtained liposome diameters are shown. Furthermore, the repeatability of liposome preparation is studied, especially upon incorporation of PEGylated lipids in the bilayer. Finally, the instruments applicability to monitor mechanical stress applied to vesicles is demonstrated.</p>},
  author       = {Weiss, Victor U. and Urey, Carlos and Gondikas, Andreas and Golesne, Monika and Friedbacher, Gernot and Von Der Kammer, Frank and Hofmann, Thilo and Andersson, Roland and Marko-Varga, György and Marchetti-Deschmann, Martina and Allmaier, Günter},
  issn         = {0003-2654},
  language     = {eng},
  month        = {11},
  number       = {21},
  pages        = {6042--6050},
  publisher    = {Royal Society of Chemistry},
  series       = {Analyst},
  title        = {Nano electrospray gas-phase electrophoretic mobility molecular analysis (nES GEMMA) of liposomes : Applicability of the technique for nano vesicle batch control},
  url          = {http://dx.doi.org/10.1039/c6an00687f},
  volume       = {141},
  year         = {2016},
}