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Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium

Pilka, Radovan LU ; Whatling, C; Domanski, Henryk LU ; Hansson, Stefan LU ; Eriksson, P and Casslén, Bertil LU (2003) In Molecular Human Reproduction 9(5). p.271-277
Abstract
The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the... (More)
The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteinase, mRNA, MMP-26, c-jun, human endometrium, regulation
in
Molecular Human Reproduction
volume
9
issue
5
pages
271 - 277
publisher
Oxford University Press
external identifiers
  • wos:000183575600005
  • pmid:12728020
  • scopus:0037635037
ISSN
1460-2407
DOI
language
English
LU publication?
yes
id
6718d6d2-b078-4b4b-beee-2e7d1a2fafc3 (old id 308427)
alternative location
http://molehr.oxfordjournals.org/cgi/content/abstract/9/5/271
date added to LUP
2007-09-20 10:24:01
date last changed
2018-05-29 09:43:43
@article{6718d6d2-b078-4b4b-beee-2e7d1a2fafc3,
  abstract     = {The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes.},
  author       = {Pilka, Radovan and Whatling, C and Domanski, Henryk and Hansson, Stefan and Eriksson, P and Casslén, Bertil},
  issn         = {1460-2407},
  keyword      = {proteinase,mRNA,MMP-26,c-jun,human endometrium,regulation},
  language     = {eng},
  number       = {5},
  pages        = {271--277},
  publisher    = {Oxford University Press},
  series       = {Molecular Human Reproduction},
  title        = {Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium},
  url          = {http://dx.doi.org/},
  volume       = {9},
  year         = {2003},
}