Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium

Pilka, Radovan LU ; Whatling, C ; Domanski, Henryk LU ; Hansson, Stefan LU orcid ; Eriksson, P and Casslén, Bertil LU (2003) In Molecular Human Reproduction 9(5). p.271-277
Abstract
The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the... (More)
The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
proteinase, mRNA, MMP-26, c-jun, human endometrium, regulation
in
Molecular Human Reproduction
volume
9
issue
5
pages
271 - 277
publisher
Oxford University Press
external identifiers
  • wos:000183575600005
  • pmid:12728020
  • scopus:0037635037
ISSN
1460-2407
DOI
10.1093/molehr/gag039
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000), Department of Obstetrics and Gynaecology (Lund) (013018000)
id
6718d6d2-b078-4b4b-beee-2e7d1a2fafc3 (old id 308427)
alternative location
http://molehr.oxfordjournals.org/cgi/content/abstract/9/5/271
date added to LUP
2016-04-01 12:23:32
date last changed
2022-01-27 03:05:25
@article{6718d6d2-b078-4b4b-beee-2e7d1a2fafc3,
  abstract     = {{The human endometrium is a dynamic tissue, which undergoes extensive tissue remodelling during the menstrual cycle. Due to their involvement in such processes, several well-characterized matrix metalloproteinases (MMP) have previously been studied in the endometrium. MMP-26 is a newly described matrilysin. We studied MMP-26 mRNA in 39 normal endometrial samples obtained across the menstrual cycle. Tissue distribution and cycle variation was examined using in-situ hybridization, Northern blot analyis and real time PCR. The probes for Northern blot analysis and real time PCR recognized non-overlapping sequences. MMP-26 was localized exclusively in epithelial cells of both glands and the luminal surface. Expression increased during the proliferative phase to a maximum at mid-cycle, then decreased to non-detectable levels in the late secretory and menstrual phases. Expression of MMP-26 mRNA in endometrial tissue explants in vitro required stimulation with both estradiol and progesterone. The tissue content of c-jun mRNA was assayed, since c-jun, as part of the enhancer complex AP-1, may be involved in regulation of MMP-26 gene transcription. The pattern of c-jun expression over the menstrual cycle was similar to that of MMP-26. Epithelial expression in the peri- and post-ovulatory stages of the menstrual cycle suggests the involvement of MMP-26 in reproductive processes.}},
  author       = {{Pilka, Radovan and Whatling, C and Domanski, Henryk and Hansson, Stefan and Eriksson, P and Casslén, Bertil}},
  issn         = {{1460-2407}},
  keywords     = {{proteinase; mRNA; MMP-26; c-jun; human endometrium; regulation}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{271--277}},
  publisher    = {{Oxford University Press}},
  series       = {{Molecular Human Reproduction}},
  title        = {{Epithelial expression of matrix metalloproteinase-26 is elevated at mid-cycle in the human endometrium}},
  url          = {{http://dx.doi.org/10.1093/molehr/gag039}},
  doi          = {{10.1093/molehr/gag039}},
  volume       = {{9}},
  year         = {{2003}},
}