Advanced

Characterization of gamma-tubulin filaments in mammalian cells

Lindström, Lisa LU and Alvarado-Kristensson, Maria LU (2018) In Biochimica et Biophysica Acta - Molecular Cell Research 1865(1). p.158-171
Abstract

Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the γ-tubulin complex proteins 2, 3, 5 and 6. Furthermore, we noted that the number of cells with cytosolic γ-tubules is increased in non-dividing cells. Our experiments showed that γ-tubules are polar structures that have a low regrowth rate compared to microtubules. Also, we observed that γ-tubules were disassembled by treatment... (More)

Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the γ-tubulin complex proteins 2, 3, 5 and 6. Furthermore, we noted that the number of cells with cytosolic γ-tubules is increased in non-dividing cells. Our experiments showed that γ-tubules are polar structures that have a low regrowth rate compared to microtubules. Also, we observed that γ-tubules were disassembled by treatment with cold, colcemid, citral dimethyl acetal, dimethyl fumarate or mutation of γ-tubulin GTPase domain, but were increased in number by treatment with taxol or by stable expression of the γ-tubulin1–333 GTPase domain. Our results demonstrate that γ-tubulin forms filaments, and such assembly is facilitated by the GTPase domain of γ-tubulin.

(Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Filaments, γ-Tubulin, γ-Tubulin ring complex
in
Biochimica et Biophysica Acta - Molecular Cell Research
volume
1865
issue
1
pages
14 pages
publisher
Elsevier
external identifiers
  • scopus:85032352376
ISSN
0167-4889
DOI
language
English
LU publication?
yes
id
309fa67f-1162-4d38-8187-2991a5d0abec
date added to LUP
2017-11-07 09:31:52
date last changed
2018-05-29 10:56:44
@article{309fa67f-1162-4d38-8187-2991a5d0abec,
  abstract     = {<p>Overexpression of γ-tubulin leads to the formation of filaments, but nothing is known about such filaments with regard to possible presence in cells, structure and probable dynamics. Here, we used mammalian cell lines to investigate the ability of γ-tubulin to form filaments. We found that γ-tubulin produces fibers called γ-tubules in a GTP-dependent manner and that γ-tubules are made up of pericentrin and the γ-tubulin complex proteins 2, 3, 5 and 6. Furthermore, we noted that the number of cells with cytosolic γ-tubules is increased in non-dividing cells. Our experiments showed that γ-tubules are polar structures that have a low regrowth rate compared to microtubules. Also, we observed that γ-tubules were disassembled by treatment with cold, colcemid, citral dimethyl acetal, dimethyl fumarate or mutation of γ-tubulin GTPase domain, but were increased in number by treatment with taxol or by stable expression of the γ-tubulin<sup>1–333</sup> GTPase domain. Our results demonstrate that γ-tubulin forms filaments, and such assembly is facilitated by the GTPase domain of γ-tubulin.</p>},
  author       = {Lindström, Lisa and Alvarado-Kristensson, Maria},
  issn         = {0167-4889},
  keyword      = {Filaments,γ-Tubulin,γ-Tubulin ring complex},
  language     = {eng},
  month        = {01},
  number       = {1},
  pages        = {158--171},
  publisher    = {Elsevier},
  series       = {Biochimica et Biophysica Acta - Molecular Cell Research},
  title        = {Characterization of gamma-tubulin filaments in mammalian cells},
  url          = {http://dx.doi.org/},
  volume       = {1865},
  year         = {2018},
}