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Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells

Desnues, B; Cuny, C; Grégori, Gérald LU ; Dukan, S; Aguilaniu, H and Nystrom, T (2003) In EMBO Reports 4(4). p.400-404
Abstract
Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by... (More)
Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by amino-acid misincorporation, proteins. Our data suggest that non-culturable cells are produced due to stochastic deterioration, rather than an adaptive programme, and pinpoint oxidation management as the 'Achilles heel' of these cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
EMBO Reports
volume
4
issue
4
pages
400 - 404
publisher
Nature Publishing Group
external identifiers
  • pmid:12671690
  • wos:000182801300014
  • scopus:0038779369
ISSN
1469-221X
DOI
10.1038/sj.embor.embor799
language
English
LU publication?
yes
id
4fd8a07d-b4d6-47bb-8291-931f167f19e3 (old id 310964)
date added to LUP
2007-08-29 11:43:28
date last changed
2018-07-01 04:19:03
@article{4fd8a07d-b4d6-47bb-8291-931f167f19e3,
  abstract     = {Potentially pathogenic bacteria, such as Escherichia coli and Vibrio cholerae, become non-culturable during stasis. The analysis of such cells has been hampered by difficulties in studying bacterial population heterogeneity. Using in situ detection of protein oxidation in single E. coli cells, and using a density-gradient centrifugation technique to separate culturable and non-culturable cells, we show that the proteins in non-culturable cells show increased and irreversible oxidative damage, which affects various bacterial compartments and proteins. The levels of expression of specific stress regulons are higher in non-culturable cells, confirming increased defects relating to oxidative damage and the occurrence of aberrant, such as by amino-acid misincorporation, proteins. Our data suggest that non-culturable cells are produced due to stochastic deterioration, rather than an adaptive programme, and pinpoint oxidation management as the 'Achilles heel' of these cells.},
  author       = {Desnues, B and Cuny, C and Grégori, Gérald and Dukan, S and Aguilaniu, H and Nystrom, T},
  issn         = {1469-221X},
  language     = {eng},
  number       = {4},
  pages        = {400--404},
  publisher    = {Nature Publishing Group},
  series       = {EMBO Reports},
  title        = {Differential oxidative damage and expression of stress defence regulons in culturable and non-culturable Escherichia coli cells},
  url          = {http://dx.doi.org/10.1038/sj.embor.embor799},
  volume       = {4},
  year         = {2003},
}