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Seed particle-enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems.

Hammarström, Björn LU ; Laurell, Thomas LU and Nilsson, Johan LU (2012) In Lab on A Chip 12(21). p.4296-4304
Abstract
Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12... (More)
Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12 micrometer-sized particles in the acoustic trap prior to the sub-micron particle capture. Furthermore, the new seeded-particle-aided acoustic trapping technique was employed for the continuous enrichment of bacteria (E. coli) with a capture efficiency of 95%. Finally, seed particle assisted acoustic trapping and enrichment is demonstrated for polymer-based particles down to 110 nm in diameter. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Lab on A Chip
volume
12
issue
21
pages
4296 - 4304
publisher
Royal Society of Chemistry
external identifiers
  • pmid:22955667
  • wos:000310916100013
  • scopus:84867287223
ISSN
1473-0189
DOI
10.1039/c2lc40697g
language
English
LU publication?
yes
id
67bf89d5-5176-4322-a9f6-73f6e4663d4c (old id 3124208)
date added to LUP
2012-10-08 11:11:01
date last changed
2017-10-01 03:21:46
@article{67bf89d5-5176-4322-a9f6-73f6e4663d4c,
  abstract     = {Acoustic trapping of sub-micron particles can allow enrichment and purification of small-sized and low-abundance microorganisms. In this paper, we investigate the dependency of the ability to capture sub-micron particles on the particle concentration. Based on the findings, it is demonstrated that seed particles can be introduced to acoustic trapping, to enable capture of low-abundance sub-micron particles. Without using seed particles, continuous enrichment of 490 nm polystyrene particles is demonstrated in a rectangular capillary with a locally generated acoustic field at high particle concentrations, i.e. above 1% wt. Trapping of sub-micron particles at significantly lower concentrations was subsequently accomplished by seeding 10-12 micrometer-sized particles in the acoustic trap prior to the sub-micron particle capture. Furthermore, the new seeded-particle-aided acoustic trapping technique was employed for the continuous enrichment of bacteria (E. coli) with a capture efficiency of 95%. Finally, seed particle assisted acoustic trapping and enrichment is demonstrated for polymer-based particles down to 110 nm in diameter.},
  author       = {Hammarström, Björn and Laurell, Thomas and Nilsson, Johan},
  issn         = {1473-0189},
  language     = {eng},
  number       = {21},
  pages        = {4296--4304},
  publisher    = {Royal Society of Chemistry},
  series       = {Lab on A Chip},
  title        = {Seed particle-enabled acoustic trapping of bacteria and nanoparticles in continuous flow systems.},
  url          = {http://dx.doi.org/10.1039/c2lc40697g},
  volume       = {12},
  year         = {2012},
}