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QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism.

Ünal, Can LU ; Singh, Birendra LU ; Fleury, Christophe LU ; Singh, Kalpana LU ; Chávez de Paz, Luis; Svensäter, Gunnel and Riesbeck, Kristian LU (2012) In International Journal of Medical Microbiology 302(6). p.261-269
Abstract
Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal... (More)
Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal violet staining. In addition, under constant flow conditions, Hi3655KR2 formed less biofilm after 48h. The biofilm defects were irrespective of autoinducer-2 levels. Hence, here we suggest for the first time a regulatory circuit in NTHi, which controls biofilm formation by mechanisms other than or in addition to luxS-dependent factors. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Medical Microbiology
volume
302
issue
6
pages
261 - 269
publisher
Elsevier
external identifiers
  • wos:000312434400005
  • pmid:22954413
  • scopus:84867858099
ISSN
1618-0607
DOI
10.1016/j.ijmm.2012.07.013
language
English
LU publication?
yes
id
390ae65f-b6e6-4c1e-bb44-a057dccb6b20 (old id 3124231)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/22954413?dopt=Abstract
date added to LUP
2012-10-04 15:44:54
date last changed
2017-10-01 03:11:43
@article{390ae65f-b6e6-4c1e-bb44-a057dccb6b20,
  abstract     = {Non-typeable Haemophilus influenzae (NTHi) is a common pathogen associated with diseases such as acute otitis media or exacerbations in patients with chronic obstructive pulmonary disease. The biofilm-forming capability substantially contributes to the persistence of NTHi. However, the regulation of biofilm formation is not completely understood. Quorum sensing regulated by autoinducer-2 produced by luxS is until now the only described regulatory mechanism. In this study, we show that the two-component signalling system QseB/C is involved in the biofilm formation of NTHi in vitro. An isogenic NTHi mutant of qseC (Hi3655KR2) showed a significant decrease in biofilm formation under static and semi-static conditions as assessed by crystal violet staining. In addition, under constant flow conditions, Hi3655KR2 formed less biofilm after 48h. The biofilm defects were irrespective of autoinducer-2 levels. Hence, here we suggest for the first time a regulatory circuit in NTHi, which controls biofilm formation by mechanisms other than or in addition to luxS-dependent factors.},
  author       = {Ünal, Can and Singh, Birendra and Fleury, Christophe and Singh, Kalpana and Chávez de Paz, Luis and Svensäter, Gunnel and Riesbeck, Kristian},
  issn         = {1618-0607},
  language     = {eng},
  number       = {6},
  pages        = {261--269},
  publisher    = {Elsevier},
  series       = {International Journal of Medical Microbiology},
  title        = {QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism.},
  url          = {http://dx.doi.org/10.1016/j.ijmm.2012.07.013},
  volume       = {302},
  year         = {2012},
}