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Ornithine decarboxylase and S-adenosylmethionine decarboxylase expression during the cell cycle of Chinese hamster ovary cells

Fredlund, J O; Johansson, M C LU ; Dahlberg, E LU and Oredsson, S M LU (1995) In Experimental Cell Research 216(1). p.86-92
Abstract

Cells in mitosis were harvested from exponentially growing Chinese hamster ovary cells by the mitotic detachment technique. Immediately after harvesting, the mitotic cells were seeded in tissue culture flasks and incubated at 37 degrees C in a CO2 incubator. Care was taken not to perturb the progression of cells through the cell cycle. At every hour after seeding for 14 h, cells were collected for analysis of cell cycle distribution, cellular polyamine content, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities, and relative mRNA contents. The progression through the cell cycle was monitored by DNA flow cytometry. The putrescine, spermidine, and spermine levels were approximately doubled during... (More)

Cells in mitosis were harvested from exponentially growing Chinese hamster ovary cells by the mitotic detachment technique. Immediately after harvesting, the mitotic cells were seeded in tissue culture flasks and incubated at 37 degrees C in a CO2 incubator. Care was taken not to perturb the progression of cells through the cell cycle. At every hour after seeding for 14 h, cells were collected for analysis of cell cycle distribution, cellular polyamine content, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities, and relative mRNA contents. The progression through the cell cycle was monitored by DNA flow cytometry. The putrescine, spermidine, and spermine levels were approximately doubled during the cell cycle: putrescine mainly during late S and G2, spermidine continuously during the entire cell cycle, and spermine mainly during G1 and S. The ODC activity was low in seeded mitotic cells and the enzyme was activated in late G1 and reached a plateau in S phase. A second burst in activity was observed during late S phase and maximal ODC activity was found at the S/G2 transition. The relative ODC mRNA level approximately doubled during the cell cycle and the increase in the relative level mainly took part during mid and late S phase. AdoMetDC activity increased in late G1 and a first maximum was observed during the G1/S transition. A second burst in activity was found in mid S phase. Maximal AdoMetDC activity was found in G2. The relative AdoMetDC mRNA approximately doubled during the cell cycle and the increase in the relative level mainly took place during late G1 and early S phase. Our results indicate that polyamine synthesis was regulated at transcriptional and translational/post-translational levels during the cell cycle of Chinese hamster ovary cells.

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published
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keywords
Adenosylmethionine Decarboxylase, Animals, CHO Cells, Cell Cycle, Cricetinae, DNA, Gene Expression Regulation, Enzymologic, Ornithine Decarboxylase, Polyamines, RNA, Messenger, Journal Article, Research Support, Non-U.S. Gov't
in
Experimental Cell Research
volume
216
issue
1
pages
7 pages
publisher
Academic Press
external identifiers
  • scopus:0028985470
ISSN
1090-2422
DOI
10.1006/excr.1995.1011http://dx.doi.org/10.1006/excr.1995.1011
language
English
LU publication?
yes
id
a0afc553-07c6-4d3b-9f03-d45a323faa84 (old id 31441)
date added to LUP
2007-06-19 14:32:10
date last changed
2017-06-19 15:12:56
@article{a0afc553-07c6-4d3b-9f03-d45a323faa84,
  abstract     = {<p>Cells in mitosis were harvested from exponentially growing Chinese hamster ovary cells by the mitotic detachment technique. Immediately after harvesting, the mitotic cells were seeded in tissue culture flasks and incubated at 37 degrees C in a CO2 incubator. Care was taken not to perturb the progression of cells through the cell cycle. At every hour after seeding for 14 h, cells were collected for analysis of cell cycle distribution, cellular polyamine content, ornithine decarboxylase (ODC) and S-adenosylmethionine decarboxylase (AdoMetDC) activities, and relative mRNA contents. The progression through the cell cycle was monitored by DNA flow cytometry. The putrescine, spermidine, and spermine levels were approximately doubled during the cell cycle: putrescine mainly during late S and G2, spermidine continuously during the entire cell cycle, and spermine mainly during G1 and S. The ODC activity was low in seeded mitotic cells and the enzyme was activated in late G1 and reached a plateau in S phase. A second burst in activity was observed during late S phase and maximal ODC activity was found at the S/G2 transition. The relative ODC mRNA level approximately doubled during the cell cycle and the increase in the relative level mainly took part during mid and late S phase. AdoMetDC activity increased in late G1 and a first maximum was observed during the G1/S transition. A second burst in activity was found in mid S phase. Maximal AdoMetDC activity was found in G2. The relative AdoMetDC mRNA approximately doubled during the cell cycle and the increase in the relative level mainly took place during late G1 and early S phase. Our results indicate that polyamine synthesis was regulated at transcriptional and translational/post-translational levels during the cell cycle of Chinese hamster ovary cells.</p>},
  author       = {Fredlund, J O and Johansson, M C and Dahlberg, E and Oredsson, S M},
  issn         = {1090-2422},
  keyword      = {Adenosylmethionine Decarboxylase,Animals,CHO Cells,Cell Cycle,Cricetinae,DNA,Gene Expression Regulation, Enzymologic,Ornithine Decarboxylase,Polyamines,RNA, Messenger,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {1},
  pages        = {86--92},
  publisher    = {Academic Press},
  series       = {Experimental Cell Research},
  title        = {Ornithine decarboxylase and S-adenosylmethionine decarboxylase expression during the cell cycle of Chinese hamster ovary cells},
  url          = {http://dx.doi.org/10.1006/excr.1995.1011http://dx.doi.org/10.1006/excr.1995.1011},
  volume       = {216},
  year         = {1995},
}