Advanced

Hyaluronic acid-collagen network interactions during the dynamic compression and recovery of cartilage

Greene, George W.; Zappone, Bruno; Banquy, Xavier; Lee, Dong Woog; Söderman, Olle LU ; Topgaard, Daniel LU and Israelachvili, Jacob N. (2012) In Soft Matter 8(38). p.9906-9914
Abstract
A compression cell designed to fit inside an NMR spectrometer was used to investigate (i) the in situ dynamic strain response and structural changes of the internal pore network, and (ii) the diffusion and flow of interstitial water, in full thickness cartilage samples as they were mechanically deformed under a constant compressive load (pressure) and then allowed to recover (swell again) when the load was removed. Selective enzymatic digestion of the collagen fibril network and the glycopolysaccharide hyaluronic acid (HA) was performed to mimic some of the structural and compositional changes associated with osteoarthritis. Digestion of collagen gave rise to mechanical 'dynamic softening' and-perhaps more importantly-nearly complete loss... (More)
A compression cell designed to fit inside an NMR spectrometer was used to investigate (i) the in situ dynamic strain response and structural changes of the internal pore network, and (ii) the diffusion and flow of interstitial water, in full thickness cartilage samples as they were mechanically deformed under a constant compressive load (pressure) and then allowed to recover (swell again) when the load was removed. Selective enzymatic digestion of the collagen fibril network and the glycopolysaccharide hyaluronic acid (HA) was performed to mimic some of the structural and compositional changes associated with osteoarthritis. Digestion of collagen gave rise to mechanical 'dynamic softening' and-perhaps more importantly-nearly complete loss in the ability to recover through swelling, both effects due to the disruption of the hierarchical structure and fibril interconnectivity in the collagen network which adversely affects its ability to deform reversibly and to properly regulate the pressurization and resulting rate and direction of interstitial fluid flow. In contrast, digestion of HA inside the collagen pore network caused the cartilage to 'dynamically stiffen' which is attributed to the decrease in the osmotic (entropic) pressure of the digested HA molecules confined in the cartilage pores that causes the network to contract and thereby become less permeable to flow. These digestion-induced changes in cartilage's properties reveal a complex relationship between the molecular weight and concentration of the HA in the interstitial fluid, and the structure and properties of the collagen fibril pore network, and provide new insights into how changes in either could influence the onset and progression of osteoarthritis. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Soft Matter
volume
8
issue
38
pages
9906 - 9914
publisher
Royal Society of Chemistry
external identifiers
  • wos:000308661700021
  • scopus:84873737839
ISSN
1744-6848
DOI
10.1039/c2sm26330k
language
English
LU publication?
yes
id
3a218eec-8a8c-4215-8e15-d86a7d365296 (old id 3146734)
date added to LUP
2012-11-01 16:49:34
date last changed
2017-03-26 03:58:00
@article{3a218eec-8a8c-4215-8e15-d86a7d365296,
  abstract     = {A compression cell designed to fit inside an NMR spectrometer was used to investigate (i) the in situ dynamic strain response and structural changes of the internal pore network, and (ii) the diffusion and flow of interstitial water, in full thickness cartilage samples as they were mechanically deformed under a constant compressive load (pressure) and then allowed to recover (swell again) when the load was removed. Selective enzymatic digestion of the collagen fibril network and the glycopolysaccharide hyaluronic acid (HA) was performed to mimic some of the structural and compositional changes associated with osteoarthritis. Digestion of collagen gave rise to mechanical 'dynamic softening' and-perhaps more importantly-nearly complete loss in the ability to recover through swelling, both effects due to the disruption of the hierarchical structure and fibril interconnectivity in the collagen network which adversely affects its ability to deform reversibly and to properly regulate the pressurization and resulting rate and direction of interstitial fluid flow. In contrast, digestion of HA inside the collagen pore network caused the cartilage to 'dynamically stiffen' which is attributed to the decrease in the osmotic (entropic) pressure of the digested HA molecules confined in the cartilage pores that causes the network to contract and thereby become less permeable to flow. These digestion-induced changes in cartilage's properties reveal a complex relationship between the molecular weight and concentration of the HA in the interstitial fluid, and the structure and properties of the collagen fibril pore network, and provide new insights into how changes in either could influence the onset and progression of osteoarthritis.},
  author       = {Greene, George W. and Zappone, Bruno and Banquy, Xavier and Lee, Dong Woog and Söderman, Olle and Topgaard, Daniel and Israelachvili, Jacob N.},
  issn         = {1744-6848},
  language     = {eng},
  number       = {38},
  pages        = {9906--9914},
  publisher    = {Royal Society of Chemistry},
  series       = {Soft Matter},
  title        = {Hyaluronic acid-collagen network interactions during the dynamic compression and recovery of cartilage},
  url          = {http://dx.doi.org/10.1039/c2sm26330k},
  volume       = {8},
  year         = {2012},
}