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Phylogenetically diverse TT virus viremia among pregnant women

Bzhalava, Davit; Ekström, Johanna LU ; Lysholm, Fredrik; Hultin, Emilie; Faust, Helena LU ; Persson, Bengt; Lehtinen, Matti; de Villiers, Ethel-Michele and Dillner, Joakim LU (2012) In Virology 432(2). p.427-434
Abstract
Infections during pregnancy have been suggested to be involved in childhood leukemias. We used high-throughput sequencing to describe the viruses most readily detectable in serum samples of pregnant women. Serum DNA of 112 mothers to leukemic children was amplified using whole genome amplification. Sequencing identified one TT virus (TTV) isolate belonging to a known type and two putatively new TTVs. For 22 mothers, we also performed ITV amplification by general primer PCR before sequencing. This detected 39 TTVs, two of which were identical to the Tilts found after whole genome amplification. Altogether, we found 40 TTV isolates, 29 of which were putatively new types (similarities ranging from 89% to 69%). In conclusion, high throughput... (More)
Infections during pregnancy have been suggested to be involved in childhood leukemias. We used high-throughput sequencing to describe the viruses most readily detectable in serum samples of pregnant women. Serum DNA of 112 mothers to leukemic children was amplified using whole genome amplification. Sequencing identified one TT virus (TTV) isolate belonging to a known type and two putatively new TTVs. For 22 mothers, we also performed ITV amplification by general primer PCR before sequencing. This detected 39 TTVs, two of which were identical to the Tilts found after whole genome amplification. Altogether, we found 40 TTV isolates, 29 of which were putatively new types (similarities ranging from 89% to 69%). In conclusion, high throughput sequencing is useful to describe the known or unknown viruses that are present in serum samples of pregnant women. (C) 2012 Elsevier Inc. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Childhood leukemia, TT virus, High throughput sequencing
in
Virology
volume
432
issue
2
pages
427 - 434
publisher
Elsevier
external identifiers
  • wos:000308121600021
  • scopus:84865280025
ISSN
1096-0341
DOI
10.1016/j.virol.2012.06.022
language
English
LU publication?
yes
id
d729baee-5c3f-4bd9-b7a0-13126e3154ba (old id 3147436)
date added to LUP
2012-11-01 09:47:15
date last changed
2017-03-19 03:54:58
@article{d729baee-5c3f-4bd9-b7a0-13126e3154ba,
  abstract     = {Infections during pregnancy have been suggested to be involved in childhood leukemias. We used high-throughput sequencing to describe the viruses most readily detectable in serum samples of pregnant women. Serum DNA of 112 mothers to leukemic children was amplified using whole genome amplification. Sequencing identified one TT virus (TTV) isolate belonging to a known type and two putatively new TTVs. For 22 mothers, we also performed ITV amplification by general primer PCR before sequencing. This detected 39 TTVs, two of which were identical to the Tilts found after whole genome amplification. Altogether, we found 40 TTV isolates, 29 of which were putatively new types (similarities ranging from 89% to 69%). In conclusion, high throughput sequencing is useful to describe the known or unknown viruses that are present in serum samples of pregnant women. (C) 2012 Elsevier Inc. All rights reserved.},
  author       = {Bzhalava, Davit and Ekström, Johanna and Lysholm, Fredrik and Hultin, Emilie and Faust, Helena and Persson, Bengt and Lehtinen, Matti and de Villiers, Ethel-Michele and Dillner, Joakim},
  issn         = {1096-0341},
  keyword      = {Childhood leukemia,TT virus,High throughput sequencing},
  language     = {eng},
  number       = {2},
  pages        = {427--434},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Phylogenetically diverse TT virus viremia among pregnant women},
  url          = {http://dx.doi.org/10.1016/j.virol.2012.06.022},
  volume       = {432},
  year         = {2012},
}