Advanced

Expression analysis of pluripotency-associated genes in human fetal cortical and striatal neural stem cells during differentiation

Massa, Denise; Pillai, Rita; Monni, Emanuela LU ; Kokaia, Zaal LU and Diana, Andrea (2012) In Translational Neuroscience 3(3). p.242-248
Abstract
In the field of developmental biology, there is compelling evidence for a network of activity of pluripotency and stem-associated genes comprising of Oct4, Nanog and nestin. During neurogenesis, the choice between enhancement versus suppression of transcriptional modulation of these identified genes determines the balance between self-renewal neural stem cells (NSC) and immature neuronal phenotypes. By using immunocytochemistry and RT-PCR techniques, our study aims to address the question whether and to what extent mRNA and protein profiles are expressed in human fetal neurospheres obtained from cortical and striatal brain regions, both in expansion (undifferentiated cells) and differentiation conditions monitored after 1 and 4 weeks in... (More)
In the field of developmental biology, there is compelling evidence for a network of activity of pluripotency and stem-associated genes comprising of Oct4, Nanog and nestin. During neurogenesis, the choice between enhancement versus suppression of transcriptional modulation of these identified genes determines the balance between self-renewal neural stem cells (NSC) and immature neuronal phenotypes. By using immunocytochemistry and RT-PCR techniques, our study aims to address the question whether and to what extent mRNA and protein profiles are expressed in human fetal neurospheres obtained from cortical and striatal brain regions, both in expansion (undifferentiated cells) and differentiation conditions monitored after 1 and 4 weeks in vitro culturing. Our results clearly demonstrate the sustained presence of opposite signals: strong downregulation of Oct4 and Nanog genes in cortical differentiating cells and significant up-regulation for nestin gene both in cortical and striatal differentiating cells. Notably, by immunostaining techniques, Oct4 and Nanog protein expression have indicated the presence of both nuclear and cytoplasmic content followed by their rapid turnover (immediately after 1 week). Moreover, during the differentiation process, dissociated neurospheres displayed unexpected number of nestin positive cells accompanied by a constant level of staining intensity. In conclusion, the present study provides new insights into brain region related features in terms of Oct4, Nanog and nestin expression both at cellular and molecular level. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Pluripotent genes, Differentiation, Cortex, Striatum, Human neurosphere, Neural stem cells
in
Translational Neuroscience
volume
3
issue
3
pages
242 - 248
publisher
Versita
external identifiers
  • wos:000308070300003
  • scopus:84871401826
ISSN
2081-6936
DOI
10.2478/s13380-012-0033-x
language
English
LU publication?
yes
id
00536071-8f5a-4cb0-b3c8-7776dc39368c (old id 3147798)
date added to LUP
2012-11-01 09:33:20
date last changed
2017-09-10 03:24:48
@article{00536071-8f5a-4cb0-b3c8-7776dc39368c,
  abstract     = {In the field of developmental biology, there is compelling evidence for a network of activity of pluripotency and stem-associated genes comprising of Oct4, Nanog and nestin. During neurogenesis, the choice between enhancement versus suppression of transcriptional modulation of these identified genes determines the balance between self-renewal neural stem cells (NSC) and immature neuronal phenotypes. By using immunocytochemistry and RT-PCR techniques, our study aims to address the question whether and to what extent mRNA and protein profiles are expressed in human fetal neurospheres obtained from cortical and striatal brain regions, both in expansion (undifferentiated cells) and differentiation conditions monitored after 1 and 4 weeks in vitro culturing. Our results clearly demonstrate the sustained presence of opposite signals: strong downregulation of Oct4 and Nanog genes in cortical differentiating cells and significant up-regulation for nestin gene both in cortical and striatal differentiating cells. Notably, by immunostaining techniques, Oct4 and Nanog protein expression have indicated the presence of both nuclear and cytoplasmic content followed by their rapid turnover (immediately after 1 week). Moreover, during the differentiation process, dissociated neurospheres displayed unexpected number of nestin positive cells accompanied by a constant level of staining intensity. In conclusion, the present study provides new insights into brain region related features in terms of Oct4, Nanog and nestin expression both at cellular and molecular level.},
  author       = {Massa, Denise and Pillai, Rita and Monni, Emanuela and Kokaia, Zaal and Diana, Andrea},
  issn         = {2081-6936},
  keyword      = {Pluripotent genes,Differentiation,Cortex,Striatum,Human neurosphere,Neural stem cells},
  language     = {eng},
  number       = {3},
  pages        = {242--248},
  publisher    = {Versita},
  series       = {Translational Neuroscience},
  title        = {Expression analysis of pluripotency-associated genes in human fetal cortical and striatal neural stem cells during differentiation},
  url          = {http://dx.doi.org/10.2478/s13380-012-0033-x},
  volume       = {3},
  year         = {2012},
}