Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma
(2003) In Journal of Infectious Diseases 187(8). p.1165-1177- Abstract
- Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients... (More)
- Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients with IM, dysplasia, and cancer. These novel findings are compatible with the hypothesis that all stages of gastric carcinogenesis are fostered by persistent intracellular expression of H. pylori virulence genes, especially cagA inside MUC2-producing precancerous gastric cells and pleomorphic cancer cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/314910
- author
- Semino-Mora, C ; Doi, SQ ; Marty, A ; Simko, V ; Carlstedt, Ingemar LU and Dubois, A
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Infectious Diseases
- volume
- 187
- issue
- 8
- pages
- 1165 - 1177
- publisher
- Oxford University Press
- external identifiers
-
- wos:000181972000001
- pmid:12695995
- scopus:0345269172
- ISSN
- 1537-6613
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Mucosal biology (013212033)
- id
- 740aec6e-5fed-4ffd-ae25-25cb9c6e6c84 (old id 314910)
- alternative location
- http://www.journals.uchicago.edu/JID/journal/issues/v187n8/21065/brief/21065.abstract.html
- date added to LUP
- 2016-04-01 16:05:23
- date last changed
- 2022-01-28 17:11:49
@article{740aec6e-5fed-4ffd-ae25-25cb9c6e6c84, abstract = {{Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients with IM, dysplasia, and cancer. These novel findings are compatible with the hypothesis that all stages of gastric carcinogenesis are fostered by persistent intracellular expression of H. pylori virulence genes, especially cagA inside MUC2-producing precancerous gastric cells and pleomorphic cancer cells.}}, author = {{Semino-Mora, C and Doi, SQ and Marty, A and Simko, V and Carlstedt, Ingemar and Dubois, A}}, issn = {{1537-6613}}, language = {{eng}}, number = {{8}}, pages = {{1165--1177}}, publisher = {{Oxford University Press}}, series = {{Journal of Infectious Diseases}}, title = {{Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma}}, url = {{http://www.journals.uchicago.edu/JID/journal/issues/v187n8/21065/brief/21065.abstract.html}}, volume = {{187}}, year = {{2003}}, }