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Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma

Semino-Mora, C; Doi, SQ; Marty, A; Simko, V; Carlstedt, Ingemar LU and Dubois, A (2003) In Journal of Infectious Diseases 187(8). p.1165-1177
Abstract
Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients... (More)
Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients with IM, dysplasia, and cancer. These novel findings are compatible with the hypothesis that all stages of gastric carcinogenesis are fostered by persistent intracellular expression of H. pylori virulence genes, especially cagA inside MUC2-producing precancerous gastric cells and pleomorphic cancer cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Infectious Diseases
volume
187
issue
8
pages
1165 - 1177
publisher
Oxford University Press
external identifiers
  • wos:000181972000001
  • pmid:12695995
  • scopus:0345269172
ISSN
1537-6613
language
English
LU publication?
yes
id
740aec6e-5fed-4ffd-ae25-25cb9c6e6c84 (old id 314910)
alternative location
http://www.journals.uchicago.edu/JID/journal/issues/v187n8/21065/brief/21065.abstract.html
date added to LUP
2007-09-21 12:26:09
date last changed
2018-10-14 04:07:20
@article{740aec6e-5fed-4ffd-ae25-25cb9c6e6c84,
  abstract     = {Gastric intestinal metaplasia (IM) and gastric cancer are associated with Helicobacter pylori, but the bacterium often is undetectable in these lesions. To unravel this apparent paradox, IM, H. pylori presence, and the expression of H. pylori virulence genes were quantified concurrently using histologic testing, in situ hybridization, and immunohistochemistry. H. pylori was detected inside metaplastic, dysplastic, and neoplastic epithelial cells, and cagA and babA2 expression was colocalized. Importantly, expression of cagA was significantly higher in patients with IM and adenocarcinoma than in control subjects. The preneoplastic "acidic" MUC2 mucin was detected only in the presence of H. pylori, and MUC2 expression was higher in patients with IM, dysplasia, and cancer. These novel findings are compatible with the hypothesis that all stages of gastric carcinogenesis are fostered by persistent intracellular expression of H. pylori virulence genes, especially cagA inside MUC2-producing precancerous gastric cells and pleomorphic cancer cells.},
  author       = {Semino-Mora, C and Doi, SQ and Marty, A and Simko, V and Carlstedt, Ingemar and Dubois, A},
  issn         = {1537-6613},
  language     = {eng},
  number       = {8},
  pages        = {1165--1177},
  publisher    = {Oxford University Press},
  series       = {Journal of Infectious Diseases},
  title        = {Intracellular and interstitial expression of Helicobacter pylori virulence genes in gastric precancerous intestinal metaplasia and adenocarcinoma},
  volume       = {187},
  year         = {2003},
}