Advanced

Phospholipase C is required for the control of stomatal aperture by ABA

Hunt, L; Mills, LN; Pical, Christophe LU ; Leckie, CP; Aitken, FL; Kopka, J; Mueller-Roeber, B; McAinsh, MR; Hetherington, AM and Gray, JE (2003) In Plant Journal 34(1). p.47-55
Abstract
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for... (More)
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
abscisic acid, guard cell, calcium, inositol 1, 4, 5-trisphosphate
in
Plant Journal
volume
34
issue
1
pages
47 - 55
publisher
Wiley-Blackwell
external identifiers
  • wos:000181862400005
  • pmid:12662308
  • scopus:0037800441
ISSN
1365-313X
DOI
10.1046/j.1365-313X.2003.01698.x
language
English
LU publication?
yes
id
e12cf620-c780-46df-adb2-25bd60e063fe (old id 315076)
date added to LUP
2007-09-13 12:13:52
date last changed
2017-04-23 04:29:17
@article{e12cf620-c780-46df-adb2-25bd60e063fe,
  abstract     = {The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.},
  author       = {Hunt, L and Mills, LN and Pical, Christophe and Leckie, CP and Aitken, FL and Kopka, J and Mueller-Roeber, B and McAinsh, MR and Hetherington, AM and Gray, JE},
  issn         = {1365-313X},
  keyword      = {abscisic acid,guard cell,calcium,inositol 1,4,5-trisphosphate},
  language     = {eng},
  number       = {1},
  pages        = {47--55},
  publisher    = {Wiley-Blackwell},
  series       = {Plant Journal},
  title        = {Phospholipase C is required for the control of stomatal aperture by ABA},
  url          = {http://dx.doi.org/10.1046/j.1365-313X.2003.01698.x},
  volume       = {34},
  year         = {2003},
}