Phospholipase C is required for the control of stomatal aperture by ABA
(2003) In Plant Journal 34(1). p.47-55- Abstract
- The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for... (More)
- The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/315076
- author
- Hunt, L ; Mills, LN ; Pical, Christophe LU ; Leckie, CP ; Aitken, FL ; Kopka, J ; Mueller-Roeber, B ; McAinsh, MR ; Hetherington, AM and Gray, JE
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- abscisic acid, guard cell, calcium, inositol 1, 4, 5-trisphosphate
- in
- Plant Journal
- volume
- 34
- issue
- 1
- pages
- 47 - 55
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000181862400005
- pmid:12662308
- scopus:0037800441
- ISSN
- 1365-313X
- DOI
- 10.1046/j.1365-313X.2003.01698.x
- language
- English
- LU publication?
- yes
- id
- e12cf620-c780-46df-adb2-25bd60e063fe (old id 315076)
- date added to LUP
- 2016-04-01 16:53:51
- date last changed
- 2022-02-28 00:27:08
@article{e12cf620-c780-46df-adb2-25bd60e063fe, abstract = {{The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.}}, author = {{Hunt, L and Mills, LN and Pical, Christophe and Leckie, CP and Aitken, FL and Kopka, J and Mueller-Roeber, B and McAinsh, MR and Hetherington, AM and Gray, JE}}, issn = {{1365-313X}}, keywords = {{abscisic acid; guard cell; calcium; inositol 1; 4; 5-trisphosphate}}, language = {{eng}}, number = {{1}}, pages = {{47--55}}, publisher = {{Wiley-Blackwell}}, series = {{Plant Journal}}, title = {{Phospholipase C is required for the control of stomatal aperture by ABA}}, url = {{http://dx.doi.org/10.1046/j.1365-313X.2003.01698.x}}, doi = {{10.1046/j.1365-313X.2003.01698.x}}, volume = {{34}}, year = {{2003}}, }