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Rapid morphological changes and loss of collagen following experimental acute colonic obstruction

Krarup, Peter-Martin; Rehn, Martin LU ; Sand-Dejmek, Janna LU ; Ehrnström, Roy LU ; Agren, Magnus S. and Syk, Ingvar LU (2013) In International Journal of Colorectal Disease 28(3). p.341-347
Abstract
Anastomosis of an acutely obstructed colon is associated with an increased risk of dehiscence. In experimental models, acute obstruction decreases collagen in the colonic wall, but the time course and propagation along the colon of the biochemical changes are unknown. Furthermore, there is a paucity of information on the correlation between these biochemical changes and histological features. Forty male Sprague Dawley rats were subjected to partial obstruction by placing a silicone ring around the left colon 30 mm above the reflection. Obstruction was maintained for 0, 1, 2, 3 or 4 days. Samples from five different locations along the colon were analysed on circumference, tissue water content, collagen concentration and histomorphology.... (More)
Anastomosis of an acutely obstructed colon is associated with an increased risk of dehiscence. In experimental models, acute obstruction decreases collagen in the colonic wall, but the time course and propagation along the colon of the biochemical changes are unknown. Furthermore, there is a paucity of information on the correlation between these biochemical changes and histological features. Forty male Sprague Dawley rats were subjected to partial obstruction by placing a silicone ring around the left colon 30 mm above the reflection. Obstruction was maintained for 0, 1, 2, 3 or 4 days. Samples from five different locations along the colon were analysed on circumference, tissue water content, collagen concentration and histomorphology. Neutrophil and macrophage infiltration was characterized immunohistochemically. The colonic circumference and water content increased (p < 0.001), while the collagen concentration decreased by 48 % (p < 0.01) proximal to the obstruction already after 1 day. The degree of dilation and collagen reduction did not change significantly over the subsequent 3 days of obstruction, whereas the water content normalized by day 3. Mucosal and submucosal oedema and the relative neutrophil infiltration were highest after 1 day in the colonic segment proximal to the stenosis while the macrophage population continued to increase to day 4. Muscular necrosis in addition to ganglionitis and neuritis in the nervous plexus increased with duration of obstruction. The pronounced and rapid changes of the composition of cells and the extracellular matrix of the colonic wall following acute obstruction may be of guidance for present surgical treatments and future pharmacological interventions. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Colonic obstruction, Collagen, Histology, Inflammation, Neutrophil, Macrophage
in
International Journal of Colorectal Disease
volume
28
issue
3
pages
341 - 347
publisher
Springer
external identifiers
  • wos:000316021300007
  • scopus:84879460266
ISSN
1432-1262
DOI
10.1007/s00384-012-1548-x
language
English
LU publication?
yes
id
31671373-5cab-4c40-b032-b664e13020f1 (old id 3657297)
date added to LUP
2013-05-02 08:53:59
date last changed
2019-04-23 01:06:54
@article{31671373-5cab-4c40-b032-b664e13020f1,
  abstract     = {Anastomosis of an acutely obstructed colon is associated with an increased risk of dehiscence. In experimental models, acute obstruction decreases collagen in the colonic wall, but the time course and propagation along the colon of the biochemical changes are unknown. Furthermore, there is a paucity of information on the correlation between these biochemical changes and histological features. Forty male Sprague Dawley rats were subjected to partial obstruction by placing a silicone ring around the left colon 30 mm above the reflection. Obstruction was maintained for 0, 1, 2, 3 or 4 days. Samples from five different locations along the colon were analysed on circumference, tissue water content, collagen concentration and histomorphology. Neutrophil and macrophage infiltration was characterized immunohistochemically. The colonic circumference and water content increased (p &lt; 0.001), while the collagen concentration decreased by 48 % (p &lt; 0.01) proximal to the obstruction already after 1 day. The degree of dilation and collagen reduction did not change significantly over the subsequent 3 days of obstruction, whereas the water content normalized by day 3. Mucosal and submucosal oedema and the relative neutrophil infiltration were highest after 1 day in the colonic segment proximal to the stenosis while the macrophage population continued to increase to day 4. Muscular necrosis in addition to ganglionitis and neuritis in the nervous plexus increased with duration of obstruction. The pronounced and rapid changes of the composition of cells and the extracellular matrix of the colonic wall following acute obstruction may be of guidance for present surgical treatments and future pharmacological interventions.},
  author       = {Krarup, Peter-Martin and Rehn, Martin and Sand-Dejmek, Janna and Ehrnström, Roy and Agren, Magnus S. and Syk, Ingvar},
  issn         = {1432-1262},
  keyword      = {Colonic obstruction,Collagen,Histology,Inflammation,Neutrophil,Macrophage},
  language     = {eng},
  number       = {3},
  pages        = {341--347},
  publisher    = {Springer},
  series       = {International Journal of Colorectal Disease},
  title        = {Rapid morphological changes and loss of collagen following experimental acute colonic obstruction},
  url          = {http://dx.doi.org/10.1007/s00384-012-1548-x},
  volume       = {28},
  year         = {2013},
}