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Distribution of respiratory mucin proteins in human nasal mucosa

Groneberg, DA; Peiser, C; Dinh, QT; Matthias, J; Eynott, PR; Heppt, W; Carlstedt, Ingemar LU ; Witt, C; Fischer, A and Chung, KF (2003) In Laryngoscope 113(3). p.520-524
Abstract
Objectives/Hypothesis: The upper respiratory tract is involved in many acute and chronic respiratory tract diseases that present with the symptom of mucus hypersecretion. Mucin genes that encode for the backbone of glycoproteins contribute to the viscoelastic property of airway mucus. We examined the cellular expression and distribution of two major respiratory mucus-forming glycoproteins, MUC5AC and MUC5B, in normal human nasal tissues. Methods: Immunohistochemical analysis using polyclonal antibodies against the mucins MUC5AC and MUC5B was performed in normal human nasal tissues. Results: An abundant staining of submucosal mucus gland and epithelial goblet cells for MUC5B was found. hnmunohistochemical analysis of MUC5AC showed staining... (More)
Objectives/Hypothesis: The upper respiratory tract is involved in many acute and chronic respiratory tract diseases that present with the symptom of mucus hypersecretion. Mucin genes that encode for the backbone of glycoproteins contribute to the viscoelastic property of airway mucus. We examined the cellular expression and distribution of two major respiratory mucus-forming glycoproteins, MUC5AC and MUC5B, in normal human nasal tissues. Methods: Immunohistochemical analysis using polyclonal antibodies against the mucins MUC5AC and MUC5B was performed in normal human nasal tissues. Results: An abundant staining of submucosal mucus gland and epithelial goblet cells for MUC5B was found. hnmunohistochemical analysis of MUC5AC showed staining of surface epithelium goblet cells, whereas there was no staining of glandular cells. Comparison of the expression to lower airways revealed a similar pattern of expression of both mucins. Conclusions: The data in the present study demonstrated the localization of the two major respiratory mucin proteins in human nasal mucosa with a similar distribution of expression of MUC5AC and MUC5B in normal upper and lower airways. Mucin protein expression parallels that of mucin messenger RNA expression. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
immunohistochemistry, MUC5B, MUC5AC, mucin, nasal mucosa
in
Laryngoscope
volume
113
issue
3
pages
520 - 524
publisher
Lippincott Williams & Wilkins
external identifiers
  • wos:000181389300023
  • pmid:12616207
  • scopus:0037337508
ISSN
1531-4995
language
English
LU publication?
yes
id
85877d38-e973-4226-ae1b-09492e7e3239 (old id 316895)
date added to LUP
2007-09-03 12:07:19
date last changed
2018-05-29 11:25:20
@article{85877d38-e973-4226-ae1b-09492e7e3239,
  abstract     = {Objectives/Hypothesis: The upper respiratory tract is involved in many acute and chronic respiratory tract diseases that present with the symptom of mucus hypersecretion. Mucin genes that encode for the backbone of glycoproteins contribute to the viscoelastic property of airway mucus. We examined the cellular expression and distribution of two major respiratory mucus-forming glycoproteins, MUC5AC and MUC5B, in normal human nasal tissues. Methods: Immunohistochemical analysis using polyclonal antibodies against the mucins MUC5AC and MUC5B was performed in normal human nasal tissues. Results: An abundant staining of submucosal mucus gland and epithelial goblet cells for MUC5B was found. hnmunohistochemical analysis of MUC5AC showed staining of surface epithelium goblet cells, whereas there was no staining of glandular cells. Comparison of the expression to lower airways revealed a similar pattern of expression of both mucins. Conclusions: The data in the present study demonstrated the localization of the two major respiratory mucin proteins in human nasal mucosa with a similar distribution of expression of MUC5AC and MUC5B in normal upper and lower airways. Mucin protein expression parallels that of mucin messenger RNA expression.},
  author       = {Groneberg, DA and Peiser, C and Dinh, QT and Matthias, J and Eynott, PR and Heppt, W and Carlstedt, Ingemar and Witt, C and Fischer, A and Chung, KF},
  issn         = {1531-4995},
  keyword      = {immunohistochemistry,MUC5B,MUC5AC,mucin,nasal mucosa},
  language     = {eng},
  number       = {3},
  pages        = {520--524},
  publisher    = {Lippincott Williams & Wilkins},
  series       = {Laryngoscope},
  title        = {Distribution of respiratory mucin proteins in human nasal mucosa},
  volume       = {113},
  year         = {2003},
}