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Feed rate control in fed-batch fermentations based on frequency content analysis.

Johnsson, Ola LU ; Andersson, Jonas LU ; Lidén, Gunnar LU ; Johnsson, Charlotta LU and Hägglund, Tore LU (2013) In Biotechnology Progress 29(3). p.817-824
Abstract
A new strategy for controlling substrate feed in the exponential growth phase of aerated fed-batch fermentations is presented. The challenge in this phase is typically to maximize specific growth rate while avoiding the accumulation of overflow metabolites which can occur at high substrate feed rates. In the new strategy, regular perturbations to the feed rate are applied and the proximity to overflow metabolism is continuously assessed from the frequency spectrum of the dissolved oxygen signal. The power spectral density for the frequency of the external perturbations is used as a control variable in a controller to regulate the substrate feed. The strategy was implemented in an industrial pilot scale fermentation set up and calibrated... (More)
A new strategy for controlling substrate feed in the exponential growth phase of aerated fed-batch fermentations is presented. The challenge in this phase is typically to maximize specific growth rate while avoiding the accumulation of overflow metabolites which can occur at high substrate feed rates. In the new strategy, regular perturbations to the feed rate are applied and the proximity to overflow metabolism is continuously assessed from the frequency spectrum of the dissolved oxygen signal. The power spectral density for the frequency of the external perturbations is used as a control variable in a controller to regulate the substrate feed. The strategy was implemented in an industrial pilot scale fermentation set up and calibrated and verified using an amylase producing Bacillus licheniformis strain. It was shown that a higher biomass yield could be obtained without excessive accumulation of harmful overflow metabolites. The general applicability of the strategy was further demonstrated by implementing the controller in another process utilizing a Bacillus licheniformis strain currently used in industrial production processes. Also in this case a higher growth rate and decreased accumulation of overflow metabolites in the exponential growth phase was achieved in comparison to the reference controller. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 2013. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biotechnology Progress
volume
29
issue
3
pages
817 - 824
publisher
The American Chemical Society
external identifiers
  • wos:000320387300025
  • pmid:23509043
  • scopus:84879267649
ISSN
1520-6033
DOI
10.1002/btpr.1727
language
English
LU publication?
yes
id
31720490-691b-41b7-a8e5-a89ca3f4f534 (old id 3627987)
date added to LUP
2013-04-10 09:47:57
date last changed
2019-02-20 01:04:12
@article{31720490-691b-41b7-a8e5-a89ca3f4f534,
  abstract     = {A new strategy for controlling substrate feed in the exponential growth phase of aerated fed-batch fermentations is presented. The challenge in this phase is typically to maximize specific growth rate while avoiding the accumulation of overflow metabolites which can occur at high substrate feed rates. In the new strategy, regular perturbations to the feed rate are applied and the proximity to overflow metabolism is continuously assessed from the frequency spectrum of the dissolved oxygen signal. The power spectral density for the frequency of the external perturbations is used as a control variable in a controller to regulate the substrate feed. The strategy was implemented in an industrial pilot scale fermentation set up and calibrated and verified using an amylase producing Bacillus licheniformis strain. It was shown that a higher biomass yield could be obtained without excessive accumulation of harmful overflow metabolites. The general applicability of the strategy was further demonstrated by implementing the controller in another process utilizing a Bacillus licheniformis strain currently used in industrial production processes. Also in this case a higher growth rate and decreased accumulation of overflow metabolites in the exponential growth phase was achieved in comparison to the reference controller. © 2013 American Institute of Chemical Engineers Biotechnol. Prog., 2013.},
  author       = {Johnsson, Ola and Andersson, Jonas and Lidén, Gunnar and Johnsson, Charlotta and Hägglund, Tore},
  issn         = {1520-6033},
  language     = {eng},
  number       = {3},
  pages        = {817--824},
  publisher    = {The American Chemical Society},
  series       = {Biotechnology Progress},
  title        = {Feed rate control in fed-batch fermentations based on frequency content analysis.},
  url          = {http://dx.doi.org/10.1002/btpr.1727},
  volume       = {29},
  year         = {2013},
}