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Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa

Grober, J; Lucas, S; Sörhede Winzell, Maria LU ; Zaghini, I; Mairal, A; Contreras, Juan Antonio LU ; Besnard, P; Holm, Cecilia LU and Langin, D (2003) In Journal of Biological Chemistry 278(8). p.6510-6515
Abstract
The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol... (More)
The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol lipase activity was unchanged in the duodenum and reduced in jejunum. Neutral cholesterol esterase activity was totally abolished in duodenum, jejunum, and ileum of HSL-null mice. Analysis of HSL mRNA structure showed two types of transcripts expressed in equal amounts with alternative 5'-ends transcribed from two exons. This work demonstrates that HSL is expressed in the mucosa of the small intestine. The results also reveal that the enzyme participates in acylglycerol hydrolysis in jejunal enterocytes and cholesteryl ester hydrolysis throughout the small intestine. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of Biological Chemistry
volume
278
issue
8
pages
6510 - 6515
publisher
ASBMB
external identifiers
  • wos:000181129400127
  • pmid:12482847
  • scopus:0037458548
ISSN
1083-351X
DOI
10.1074/jbc.M208513200
language
English
LU publication?
yes
id
475dc527-78a7-48f3-a841-ddb1312ad869 (old id 317508)
date added to LUP
2007-09-03 12:03:44
date last changed
2017-08-06 03:47:25
@article{475dc527-78a7-48f3-a841-ddb1312ad869,
  abstract     = {The identity of the enzymes responsible for lipase and cholesterol esterase activities in the small intestinal mucosa is not known. Because hormone-sensitive lipase (HSL) catalyzes the hydrolysis of acylglycerols and cholesteryl esters, we sought to determine whether HSL could be involved. HSL mRNA and protein were detected in all segments of the small intestine by Northern and Western blot analyses, respectively. Immunocytochemistry experiments revealed that HSL was expressed in the differentiated enterocytes of the villi and was absent in the undifferentiated cells of the crypt. Diacylglycerol lipase and cholesterol esterase activities were found in the different segments. Analysis of gut from HSL-null mice showed that diacylglycerol lipase activity was unchanged in the duodenum and reduced in jejunum. Neutral cholesterol esterase activity was totally abolished in duodenum, jejunum, and ileum of HSL-null mice. Analysis of HSL mRNA structure showed two types of transcripts expressed in equal amounts with alternative 5'-ends transcribed from two exons. This work demonstrates that HSL is expressed in the mucosa of the small intestine. The results also reveal that the enzyme participates in acylglycerol hydrolysis in jejunal enterocytes and cholesteryl ester hydrolysis throughout the small intestine.},
  author       = {Grober, J and Lucas, S and Sörhede Winzell, Maria and Zaghini, I and Mairal, A and Contreras, Juan Antonio and Besnard, P and Holm, Cecilia and Langin, D},
  issn         = {1083-351X},
  language     = {eng},
  number       = {8},
  pages        = {6510--6515},
  publisher    = {ASBMB},
  series       = {Journal of Biological Chemistry},
  title        = {Hormone-sensitive lipase is a cholesterol esterase of the intestinal mucosa},
  url          = {http://dx.doi.org/10.1074/jbc.M208513200},
  volume       = {278},
  year         = {2003},
}