Dual R3R5 tropism characterizes cerebrospinal fluid HIV-1 isolates from individuals with high cerebrospinal fluid viral load
(2012) In AIDS 26(14). p.1739-1744- Abstract
- Objective: To study the use of major and alternative coreceptors by HIV-1 isolates obtained from paired plasma and cerebrospinal fluid (CSF) samples. Design: Paired plasma and CSF isolates from HIV-1-infected individuals with varying clinical, virologic, and immunologic parameters were assessed for the ability to infect indicator cells expressing a panel of coreceptors with documented expression in the central nervous system (CNS). Methods: HIV-1 isolates obtained from plasma and CSF in 28 individuals with varying viral load, CD4(+) T-cell counts, and with or without AIDS-defining disease were analyzed for the ability to infect NP2.CD4 cells stably expressing a panel of HIV coreceptors (CCR5, CXCR4, CCR3, CXCR6, GPR1, APJ, ChemR23, RDC-1... (More)
- Objective: To study the use of major and alternative coreceptors by HIV-1 isolates obtained from paired plasma and cerebrospinal fluid (CSF) samples. Design: Paired plasma and CSF isolates from HIV-1-infected individuals with varying clinical, virologic, and immunologic parameters were assessed for the ability to infect indicator cells expressing a panel of coreceptors with documented expression in the central nervous system (CNS). Methods: HIV-1 isolates obtained from plasma and CSF in 28 individuals with varying viral load, CD4(+) T-cell counts, and with or without AIDS-defining disease were analyzed for the ability to infect NP2.CD4 cells stably expressing a panel of HIV coreceptors (CCR5, CXCR4, CCR3, CXCR6, GPR1, APJ, ChemR23, RDC-1 or BLT1). Results: All isolates from both plasma and CSF utilized CCR5 and/or CXCR4. However, the ability to use both CCR3 and CCR5 (R3R5) was more pronounced in CSF isolates and correlated with high CSF viral load and low CD4(+) T-cell count. Notably, four out of five CSF isolates of subtype C origin exhibited CXCR6 use, which coincided with high CSF viral load despite preserved CD4(+) T-cell counts. The use of other alternative coreceptors was less pronounced. Conclusion: Dual-tropic R3R5 HIV-1 isolates in CSF coincide with high CSF viral load and low CD4(+) T-cell counts. Frequent CXCR6 use by CSF-derived subtype C isolates indicates that subtype-specific differences in coreceptor use may exist that will not be acknowledged when assessing plasma virus isolates. The findings may also bare relevance for HIV-1 replication within the CNS, and consequently, for the neuropathogenesis of AIDS. (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3191196
- author
- Karlsson, Ulf LU ; Antonsson, Liselotte LU ; Ljungberg, Bengt LU ; Medstrand, Patrik LU ; Esbjörnsson, Joakim LU ; Jansson, Marianne LU and Gisslen, Magnus
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- CCR3, cerebrospinal fluid viral load, CXCR6, HIV-1 R5, late stage, disease, subtype C
- in
- AIDS
- volume
- 26
- issue
- 14
- pages
- 1739 - 1744
- publisher
- Lippincott Williams & Wilkins
- external identifiers
-
- wos:000308683700002
- pmid:22695299
- scopus:84866317711
- pmid:22695299
- ISSN
- 1473-5571
- DOI
- 10.1097/QAD.0b013e3283560791
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Drug Target Discovery (013212045), Molecular Virology (013212007), Division of Medical Microbiology (013250400), Division of Infection Medicine (SUS) (013008000)
- id
- 7bf7d077-651c-4cf5-8ac1-233e598d32a1 (old id 3191196)
- date added to LUP
- 2016-04-01 11:06:26
- date last changed
- 2022-01-26 05:27:37
@article{7bf7d077-651c-4cf5-8ac1-233e598d32a1, abstract = {{Objective: To study the use of major and alternative coreceptors by HIV-1 isolates obtained from paired plasma and cerebrospinal fluid (CSF) samples. Design: Paired plasma and CSF isolates from HIV-1-infected individuals with varying clinical, virologic, and immunologic parameters were assessed for the ability to infect indicator cells expressing a panel of coreceptors with documented expression in the central nervous system (CNS). Methods: HIV-1 isolates obtained from plasma and CSF in 28 individuals with varying viral load, CD4(+) T-cell counts, and with or without AIDS-defining disease were analyzed for the ability to infect NP2.CD4 cells stably expressing a panel of HIV coreceptors (CCR5, CXCR4, CCR3, CXCR6, GPR1, APJ, ChemR23, RDC-1 or BLT1). Results: All isolates from both plasma and CSF utilized CCR5 and/or CXCR4. However, the ability to use both CCR3 and CCR5 (R3R5) was more pronounced in CSF isolates and correlated with high CSF viral load and low CD4(+) T-cell count. Notably, four out of five CSF isolates of subtype C origin exhibited CXCR6 use, which coincided with high CSF viral load despite preserved CD4(+) T-cell counts. The use of other alternative coreceptors was less pronounced. Conclusion: Dual-tropic R3R5 HIV-1 isolates in CSF coincide with high CSF viral load and low CD4(+) T-cell counts. Frequent CXCR6 use by CSF-derived subtype C isolates indicates that subtype-specific differences in coreceptor use may exist that will not be acknowledged when assessing plasma virus isolates. The findings may also bare relevance for HIV-1 replication within the CNS, and consequently, for the neuropathogenesis of AIDS. (C) 2012 Wolters Kluwer Health vertical bar Lippincott Williams & Wilkins}}, author = {{Karlsson, Ulf and Antonsson, Liselotte and Ljungberg, Bengt and Medstrand, Patrik and Esbjörnsson, Joakim and Jansson, Marianne and Gisslen, Magnus}}, issn = {{1473-5571}}, keywords = {{CCR3; cerebrospinal fluid viral load; CXCR6; HIV-1 R5; late stage; disease; subtype C}}, language = {{eng}}, number = {{14}}, pages = {{1739--1744}}, publisher = {{Lippincott Williams & Wilkins}}, series = {{AIDS}}, title = {{Dual R3R5 tropism characterizes cerebrospinal fluid HIV-1 isolates from individuals with high cerebrospinal fluid viral load}}, url = {{http://dx.doi.org/10.1097/QAD.0b013e3283560791}}, doi = {{10.1097/QAD.0b013e3283560791}}, volume = {{26}}, year = {{2012}}, }