Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Solubility of Aβ40 peptide

Lattanzi, Veronica LU ; Bernfur, Katja LU ; Sparr, Emma LU ; Olsson, Ulf LU and Linse, Sara LU (2021) In JCIS Open 4.
Abstract

In this work we measured, by using a direct approach, the equilibrium solubility of recombinant Aβ40 peptide to be S ​= ​0.36 ​± ​0.15 ​μM in aqueous solution of 20 ​mM sodium phosphate buffer at pH 7.4. Microfluidic diffusional sizing (MDS) and mass spectrometry (MALDI-TOF/TOF) with isotope standard were used to quantify the concentration of soluble Aβ40 species coexisting with formed fibrils. A sample set of Aβ40 at different concentrations was incubated at 37 ​°C under quiescent conditions and for different incubation times. After incubation, the samples were centrifuged and the concentration of Aβ40 in the supernatant was determined. This allowed us to also determine the metastable zone in supersaturated Aβ40 monomer solutions, as a... (More)

In this work we measured, by using a direct approach, the equilibrium solubility of recombinant Aβ40 peptide to be S ​= ​0.36 ​± ​0.15 ​μM in aqueous solution of 20 ​mM sodium phosphate buffer at pH 7.4. Microfluidic diffusional sizing (MDS) and mass spectrometry (MALDI-TOF/TOF) with isotope standard were used to quantify the concentration of soluble Aβ40 species coexisting with formed fibrils. A sample set of Aβ40 at different concentrations was incubated at 37 ​°C under quiescent conditions and for different incubation times. After incubation, the samples were centrifuged and the concentration of Aβ40 in the supernatant was determined. This allowed us to also determine the metastable zone in supersaturated Aβ40 monomer solutions, as a function of the observation time, as well as the monomer concentration coexisting with formed fibrils. Moreover, the hydrodynamic radius (RH) of the Aβ40 species detected in the supernatant at different time points was also measured, and showed a constant value of ∼1.8 ​nm, consistent with random coil Aβ40 monomers.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Alzheimer, Mass spectrometry, Metastable regime, Microfluidics diffusion sizing, Recombinant amyloid beta 40, Solubility
in
JCIS Open
volume
4
article number
100024
publisher
Elsevier
external identifiers
  • scopus:85126581106
DOI
10.1016/j.jciso.2021.100024
language
English
LU publication?
yes
id
3191eabf-8b70-4bf7-bf90-a3742265923f
date added to LUP
2022-05-02 15:50:01
date last changed
2023-11-21 04:27:14
@article{3191eabf-8b70-4bf7-bf90-a3742265923f,
  abstract     = {{<p>In this work we measured, by using a direct approach, the equilibrium solubility of recombinant Aβ40 peptide to be S ​= ​0.36 ​± ​0.15 ​μM in aqueous solution of 20 ​mM sodium phosphate buffer at pH 7.4. Microfluidic diffusional sizing (MDS) and mass spectrometry (MALDI-TOF/TOF) with isotope standard were used to quantify the concentration of soluble Aβ40 species coexisting with formed fibrils. A sample set of Aβ40 at different concentrations was incubated at 37 ​°C under quiescent conditions and for different incubation times. After incubation, the samples were centrifuged and the concentration of Aβ40 in the supernatant was determined. This allowed us to also determine the metastable zone in supersaturated Aβ40 monomer solutions, as a function of the observation time, as well as the monomer concentration coexisting with formed fibrils. Moreover, the hydrodynamic radius (R<sub>H</sub>) of the Aβ40 species detected in the supernatant at different time points was also measured, and showed a constant value of ∼1.8 ​nm, consistent with random coil Aβ40 monomers.</p>}},
  author       = {{Lattanzi, Veronica and Bernfur, Katja and Sparr, Emma and Olsson, Ulf and Linse, Sara}},
  keywords     = {{Alzheimer; Mass spectrometry; Metastable regime; Microfluidics diffusion sizing; Recombinant amyloid beta 40; Solubility}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{JCIS Open}},
  title        = {{Solubility of Aβ40 peptide}},
  url          = {{http://dx.doi.org/10.1016/j.jciso.2021.100024}},
  doi          = {{10.1016/j.jciso.2021.100024}},
  volume       = {{4}},
  year         = {{2021}},
}