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Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms.

Ahmad, Khadija Mohamed LU ; Ishchuk, Olena LU ; Hellborg, Linda LU ; Jørgensen, Gloria ; Skvarc, Miha ; Stenderup, Jørgen ; Jørck-Ramberg, Dorte ; Poláková, Silvia LU and Piskur, Jure LU (2013) In Antonie van Leeuwenhoek 104(1). p.111-122
Abstract
We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985-1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, these C. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to each other (i.e. they contained different sets of genes). We suggest that at least two mechanisms could participate in their origin: (i) through a segmental duplication which covered the centromeric region, or (ii) by a translocation event moving a... (More)
We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985-1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, these C. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to each other (i.e. they contained different sets of genes). We suggest that at least two mechanisms could participate in their origin: (i) through a segmental duplication which covered the centromeric region, or (ii) by a translocation event moving a larger chromosome arm to another chromosome that leaves the centromere part with the shorter arm. The first type of small chromosomes carrying duplicated genes exhibited mitotic instability, while the second type, which contained the corresponding genes in only one copy in the genome, was mitotically stable. Apparently, in patients C. glabrata chromosomes are frequently reshuffled resulting in new genetic configurations, including appearance of small chromosomes, and some of these resulting "mutant" strains can have increased fitness in a certain patient "environment". (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Phylogeny, Phatogenic yeast, Chromosome, Genome rearrangements
in
Antonie van Leeuwenhoek
volume
104
issue
1
pages
111 - 122
publisher
Springer
external identifiers
  • pmid:23670790
  • wos:000319992200011
  • scopus:84878879818
  • pmid:23670790
ISSN
1572-9699
DOI
10.1007/s10482-013-9931-3
language
English
LU publication?
yes
id
31d83c89-b501-4d64-ae52-d41aa4fb2018 (old id 3804477)
date added to LUP
2016-04-01 10:42:40
date last changed
2020-01-12 05:10:37
@article{31d83c89-b501-4d64-ae52-d41aa4fb2018,
  abstract     = {We analyzed 192 strains of the pathogenic yeast Candida glabrata from patients, mainly suffering from systemic infection, at Danish hospitals during 1985-1999. Our analysis showed that these strains were closely related but exhibited large karyotype polymorphism. Nine strains contained small chromosomes, which were smaller than 0.5 Mb. Regarding the year, patient and hospital, these C. glabrata strains had independent origin and the analyzed small chromosomes were structurally not related to each other (i.e. they contained different sets of genes). We suggest that at least two mechanisms could participate in their origin: (i) through a segmental duplication which covered the centromeric region, or (ii) by a translocation event moving a larger chromosome arm to another chromosome that leaves the centromere part with the shorter arm. The first type of small chromosomes carrying duplicated genes exhibited mitotic instability, while the second type, which contained the corresponding genes in only one copy in the genome, was mitotically stable. Apparently, in patients C. glabrata chromosomes are frequently reshuffled resulting in new genetic configurations, including appearance of small chromosomes, and some of these resulting "mutant" strains can have increased fitness in a certain patient "environment".},
  author       = {Ahmad, Khadija Mohamed and Ishchuk, Olena and Hellborg, Linda and Jørgensen, Gloria and Skvarc, Miha and Stenderup, Jørgen and Jørck-Ramberg, Dorte and Poláková, Silvia and Piskur, Jure},
  issn         = {1572-9699},
  language     = {eng},
  number       = {1},
  pages        = {111--122},
  publisher    = {Springer},
  series       = {Antonie van Leeuwenhoek},
  title        = {Small chromosomes among Danish Candida glabrata isolates originated through different mechanisms.},
  url          = {http://dx.doi.org/10.1007/s10482-013-9931-3},
  doi          = {10.1007/s10482-013-9931-3},
  volume       = {104},
  year         = {2013},
}