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Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines

Patzke, S; Lindeskog, Mats LU ; Munthe, E and Aasheim, HC (2002) In Virology 303(1). p.164-173
Abstract
We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression... (More)
We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression pattern. The 5' long terminal repeat (LTR) was tested for promoter activity in HERV-H/F expressing and nonexpressing cell lines. The cell specificity of the LTR-mediated reporter gene expression did not conclusively correlate with endogenous virus expression, indicating that the transcription regulation of this gene is not alone dependent on cell-specific activity of transcription factors. (C) 2002 Elsevier Science (USA). (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
LTR, leukemia, HERV, endogenous retrovirus, expression
in
Virology
volume
303
issue
1
pages
164 - 173
publisher
Elsevier
external identifiers
  • wos:000179828800016
  • pmid:12482668
  • scopus:0036924747
ISSN
1096-0341
DOI
10.1006/viro.2002.1615
language
English
LU publication?
yes
id
1ce6fd9f-c3ba-4aed-a84b-7ffe482bbb08 (old id 321462)
date added to LUP
2007-11-08 10:58:26
date last changed
2017-08-06 04:41:39
@article{1ce6fd9f-c3ba-4aed-a84b-7ffe482bbb08,
  abstract     = {We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression pattern. The 5' long terminal repeat (LTR) was tested for promoter activity in HERV-H/F expressing and nonexpressing cell lines. The cell specificity of the LTR-mediated reporter gene expression did not conclusively correlate with endogenous virus expression, indicating that the transcription regulation of this gene is not alone dependent on cell-specific activity of transcription factors. (C) 2002 Elsevier Science (USA).},
  author       = {Patzke, S and Lindeskog, Mats and Munthe, E and Aasheim, HC},
  issn         = {1096-0341},
  keyword      = {LTR,leukemia,HERV,endogenous retrovirus,expression},
  language     = {eng},
  number       = {1},
  pages        = {164--173},
  publisher    = {Elsevier},
  series       = {Virology},
  title        = {Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines},
  url          = {http://dx.doi.org/10.1006/viro.2002.1615},
  volume       = {303},
  year         = {2002},
}