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Microdialysis in Early Detection of Temporary Pancreatic Ischemia in a Porcine Model.

Blind, Per-Jonas LU ; Kral, J; Wang, Wei LU ; Kralova, I; Abrahamsson, Per-Anders LU ; Johansson, G and Winsö, O (2012) In European Surgical Research 49(3-4). p.113-120
Abstract
Background: Ischemic injury to the pancreas occurs in various clinical conditions. A method for online monitoring of pathophysiological events in pancreatic parenchyma is missing. Aims: To assess the timing of microdialysis (MD) technique response on temporary changes in pancreatic perfusion, and to evaluate the relationship between MD data and systemic markers of anaerobic metabolism and inflammation. Methods: In anaesthetized normoventilated pigs, MD probes were placed in right (control) and left (ischemic) pancreatic lobes, respectively. Following the clamping of the vessels, ischemia was verified by tissue oxygen tension (P(ti)O(2)) measurements. Results: P(ti)O(2) decreased within 20 min after the clamping of the vessels, already... (More)
Background: Ischemic injury to the pancreas occurs in various clinical conditions. A method for online monitoring of pathophysiological events in pancreatic parenchyma is missing. Aims: To assess the timing of microdialysis (MD) technique response on temporary changes in pancreatic perfusion, and to evaluate the relationship between MD data and systemic markers of anaerobic metabolism and inflammation. Methods: In anaesthetized normoventilated pigs, MD probes were placed in right (control) and left (ischemic) pancreatic lobes, respectively. Following the clamping of the vessels, ischemia was verified by tissue oxygen tension (P(ti)O(2)) measurements. Results: P(ti)O(2) decreased within 20 min after the clamping of the vessels, already returning to baseline levels at the first sampling point after the removal of the clamp. MD lactate levels increased, whereas pyruvate and glucose levels decreased at 20 min after the induction of ischemia. These trends continued until the end of ischemia and returned to baseline following reperfusion. Serum lactate, amylase and tumor necrosis factor-alpha levels decreased throughout the protocol time. Conclusion: MD data were in concordance with changes in P(ti)O(2), which is indicative of local anaerobic metabolism. MD allowed the detection of pathophysiological processes within the ischemic pancreas at a stage when no elevations of systemic markers of ischemia or inflammation were observed. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
European Surgical Research
volume
49
issue
3-4
pages
113 - 120
publisher
Karger
external identifiers
  • pmid:23171994
  • wos:000312589900003
  • scopus:84871559250
ISSN
0014-312X
DOI
10.1159/000343806
language
English
LU publication?
yes
id
cf496998-1102-4eac-850d-814224985b6b (old id 3218624)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/23171994?dopt=Abstract
date added to LUP
2012-12-03 21:20:59
date last changed
2017-01-01 07:37:16
@article{cf496998-1102-4eac-850d-814224985b6b,
  abstract     = {Background: Ischemic injury to the pancreas occurs in various clinical conditions. A method for online monitoring of pathophysiological events in pancreatic parenchyma is missing. Aims: To assess the timing of microdialysis (MD) technique response on temporary changes in pancreatic perfusion, and to evaluate the relationship between MD data and systemic markers of anaerobic metabolism and inflammation. Methods: In anaesthetized normoventilated pigs, MD probes were placed in right (control) and left (ischemic) pancreatic lobes, respectively. Following the clamping of the vessels, ischemia was verified by tissue oxygen tension (P(ti)O(2)) measurements. Results: P(ti)O(2) decreased within 20 min after the clamping of the vessels, already returning to baseline levels at the first sampling point after the removal of the clamp. MD lactate levels increased, whereas pyruvate and glucose levels decreased at 20 min after the induction of ischemia. These trends continued until the end of ischemia and returned to baseline following reperfusion. Serum lactate, amylase and tumor necrosis factor-alpha levels decreased throughout the protocol time. Conclusion: MD data were in concordance with changes in P(ti)O(2), which is indicative of local anaerobic metabolism. MD allowed the detection of pathophysiological processes within the ischemic pancreas at a stage when no elevations of systemic markers of ischemia or inflammation were observed.},
  author       = {Blind, Per-Jonas and Kral, J and Wang, Wei and Kralova, I and Abrahamsson, Per-Anders and Johansson, G and Winsö, O},
  issn         = {0014-312X},
  language     = {eng},
  number       = {3-4},
  pages        = {113--120},
  publisher    = {Karger},
  series       = {European Surgical Research},
  title        = {Microdialysis in Early Detection of Temporary Pancreatic Ischemia in a Porcine Model.},
  url          = {http://dx.doi.org/10.1159/000343806},
  volume       = {49},
  year         = {2012},
}