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Supported liquid membrane extraction for identification of phenolic compounds in the nutrient solution of closed hydroponic growing systems for tomato

Jung, V; Chimuka, L; Jönsson, Jan Åke LU ; Niedack, N; Bowens, P and Alsanius, B (2002) In Analytica Chimica Acta 474(1-2). p.49-57
Abstract
The objective of the present study was to adapt and develop suitable methods to study the occurrence metabolites in the nutrient solution of hydroponically grown tomato. The organic-chemical nature of the nutrient solution from tomato grown in closed hydroponic systems was studied by supported liquid membrane technique (SLM) and analyzed by high-performance liquid chromatography (HPLC). Further, we aimed to identify and quantify metabolites present the growing system. In this context, the analyses focused on benzoic acid, ferulic acid, p-hydroxybenzoic acid, salicylic acid, vanillic acid. and phenazine-1-carboxylic acid. Results showed that supported liquid membrane technique is a suitable tool for analyzing nutrient solution of closed... (More)
The objective of the present study was to adapt and develop suitable methods to study the occurrence metabolites in the nutrient solution of hydroponically grown tomato. The organic-chemical nature of the nutrient solution from tomato grown in closed hydroponic systems was studied by supported liquid membrane technique (SLM) and analyzed by high-performance liquid chromatography (HPLC). Further, we aimed to identify and quantify metabolites present the growing system. In this context, the analyses focused on benzoic acid, ferulic acid, p-hydroxybenzoic acid, salicylic acid, vanillic acid. and phenazine-1-carboxylic acid. Results showed that supported liquid membrane technique is a suitable tool for analyzing nutrient solution of closed hydroponically grown tomatoes, with respect to the studied compounds. Both extraction efficiency and detection limits varied between the compounds. To enable all compounds to be seen in one chromatogram a gradient was used for high-performance liquid chromatography. Agreement of retention times for standards and samples showed occurrence of vanillic acid, ferulic acid, phenazine and p-hydroxybenzoic acid. Vanillic acid was present in concentrations around 60 nM in almost all samples. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
high-performance liquid chromatography, nutrient film technique, phenolic acids, rockwool
in
Analytica Chimica Acta
volume
474
issue
1-2
pages
49 - 57
publisher
Elsevier
external identifiers
  • wos:000179404300006
  • scopus:0037049304
ISSN
1873-4324
DOI
10.1016/S0003-2670(02)01013-9
language
English
LU publication?
yes
id
50285a7a-da95-4e22-93af-ac3898a222ce (old id 322920)
date added to LUP
2007-10-31 14:58:42
date last changed
2017-01-01 07:06:23
@article{50285a7a-da95-4e22-93af-ac3898a222ce,
  abstract     = {The objective of the present study was to adapt and develop suitable methods to study the occurrence metabolites in the nutrient solution of hydroponically grown tomato. The organic-chemical nature of the nutrient solution from tomato grown in closed hydroponic systems was studied by supported liquid membrane technique (SLM) and analyzed by high-performance liquid chromatography (HPLC). Further, we aimed to identify and quantify metabolites present the growing system. In this context, the analyses focused on benzoic acid, ferulic acid, p-hydroxybenzoic acid, salicylic acid, vanillic acid. and phenazine-1-carboxylic acid. Results showed that supported liquid membrane technique is a suitable tool for analyzing nutrient solution of closed hydroponically grown tomatoes, with respect to the studied compounds. Both extraction efficiency and detection limits varied between the compounds. To enable all compounds to be seen in one chromatogram a gradient was used for high-performance liquid chromatography. Agreement of retention times for standards and samples showed occurrence of vanillic acid, ferulic acid, phenazine and p-hydroxybenzoic acid. Vanillic acid was present in concentrations around 60 nM in almost all samples.},
  author       = {Jung, V and Chimuka, L and Jönsson, Jan Åke and Niedack, N and Bowens, P and Alsanius, B},
  issn         = {1873-4324},
  keyword      = {high-performance liquid chromatography,nutrient film technique,phenolic acids,rockwool},
  language     = {eng},
  number       = {1-2},
  pages        = {49--57},
  publisher    = {Elsevier},
  series       = {Analytica Chimica Acta},
  title        = {Supported liquid membrane extraction for identification of phenolic compounds in the nutrient solution of closed hydroponic growing systems for tomato},
  url          = {http://dx.doi.org/10.1016/S0003-2670(02)01013-9},
  volume       = {474},
  year         = {2002},
}