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Effects of tamoxifen and estradiol on estrogen binding sites in the urogenital tract: an experimental study in the rabbit

Iosif, CS; Al-Hijji, J and Samsioe, Göran LU (2002) In Gynecological Endocrinology 16(5). p.379-383
Abstract
The aim of the study was to investigate the effects of estradiol and tamoxifen alone and in combination on the estrogen binding site status of the urogenital tract in the rabbit. Bilaterally ovariectomized rabbits were divided into four groups of six. Wereas the control group received no treatment, the remaining rabbits were treated with estrogen orland tamoxifen. Cytosolic and nuclear fractions were isolated from the uterus, vagina, urethra and urinary bladder and used for binding site assay, by radioligand binding. The total weight of the rabbit vagina and uterus was increased significantly by both estradiol, tamoxifen and the combination of the two. The total weight of the urethra was increased only in the combination group. The cytosol... (More)
The aim of the study was to investigate the effects of estradiol and tamoxifen alone and in combination on the estrogen binding site status of the urogenital tract in the rabbit. Bilaterally ovariectomized rabbits were divided into four groups of six. Wereas the control group received no treatment, the remaining rabbits were treated with estrogen orland tamoxifen. Cytosolic and nuclear fractions were isolated from the uterus, vagina, urethra and urinary bladder and used for binding site assay, by radioligand binding. The total weight of the rabbit vagina and uterus was increased significantly by both estradiol, tamoxifen and the combination of the two. The total weight of the urethra was increased only in the combination group. The cytosol binding site was downregulated by estradiol, tamoxifen and combination in the uterus, and in the vagina. Cytosol binding site in the urethra was not detected. The combination of estrogen-tamoxifen markedly reduced the nuclear binding site in the urethra and decreased affinity of the nuclear binding sites in all three tissues. The data suggest that tamoxifen has a specific ability to modulate the transcriptional activity of the estrogen binding sites in the rabbit urogenital tract. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
tamoxifen, estrogen, urogenital tract, animal experiment, rabbit, estrogen binding site
in
Gynecological Endocrinology
volume
16
issue
5
pages
379 - 383
publisher
Informa Healthcare
external identifiers
  • wos:000179385600006
  • pmid:12587532
  • scopus:0036813301
ISSN
0951-3590
language
English
LU publication?
yes
id
02e58232-acac-40b7-8002-256264c6918b (old id 323520)
alternative location
http://www.ingentaconnect.com/content/tandf/ge/2002/00000016/00000005/art00006
date added to LUP
2007-10-23 09:19:29
date last changed
2017-01-01 05:15:17
@article{02e58232-acac-40b7-8002-256264c6918b,
  abstract     = {The aim of the study was to investigate the effects of estradiol and tamoxifen alone and in combination on the estrogen binding site status of the urogenital tract in the rabbit. Bilaterally ovariectomized rabbits were divided into four groups of six. Wereas the control group received no treatment, the remaining rabbits were treated with estrogen orland tamoxifen. Cytosolic and nuclear fractions were isolated from the uterus, vagina, urethra and urinary bladder and used for binding site assay, by radioligand binding. The total weight of the rabbit vagina and uterus was increased significantly by both estradiol, tamoxifen and the combination of the two. The total weight of the urethra was increased only in the combination group. The cytosol binding site was downregulated by estradiol, tamoxifen and combination in the uterus, and in the vagina. Cytosol binding site in the urethra was not detected. The combination of estrogen-tamoxifen markedly reduced the nuclear binding site in the urethra and decreased affinity of the nuclear binding sites in all three tissues. The data suggest that tamoxifen has a specific ability to modulate the transcriptional activity of the estrogen binding sites in the rabbit urogenital tract.},
  author       = {Iosif, CS and Al-Hijji, J and Samsioe, Göran},
  issn         = {0951-3590},
  keyword      = {tamoxifen,estrogen,urogenital tract,animal experiment,rabbit,estrogen binding site},
  language     = {eng},
  number       = {5},
  pages        = {379--383},
  publisher    = {Informa Healthcare},
  series       = {Gynecological Endocrinology},
  title        = {Effects of tamoxifen and estradiol on estrogen binding sites in the urogenital tract: an experimental study in the rabbit},
  volume       = {16},
  year         = {2002},
}