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Size separation of supermicrometer particles in asymmetrical flow field-flow fractionation. Flow conditions for rapid elution

Wahlund, Karl-Gustav LU and Zattoni, A (2002) In Analytical Chemistry 74(21). p.5621-5628
Abstract
The performance of lift-hyperlayer asymmetrical flow field-flow fractionation using rapid elution conditions was tested through the separation of standard polystyrene latex particles of diameters from 2 to 20 mum. Optimization of flowrates was studied not only in order to obtain efficient and rapid separation; but also to work under conditions of various shape and steepness of the axial flow velocity gradient. Using extreme flow conditions, the five widely spaced particle sizes, 20.5-, 15.0-, 9.7-, 5.0-, and 2.0-mum diameter, could be resolved in 6 min, whereas for the narrower size range of 20.5-5.0 mum, 1 min was enough. The size selectivity in the size range 9.7-2.0 mum was studied as a function of flowrates and particle size and was... (More)
The performance of lift-hyperlayer asymmetrical flow field-flow fractionation using rapid elution conditions was tested through the separation of standard polystyrene latex particles of diameters from 2 to 20 mum. Optimization of flowrates was studied not only in order to obtain efficient and rapid separation; but also to work under conditions of various shape and steepness of the axial flow velocity gradient. Using extreme flow conditions, the five widely spaced particle sizes, 20.5-, 15.0-, 9.7-, 5.0-, and 2.0-mum diameter, could be resolved in 6 min, whereas for the narrower size range of 20.5-5.0 mum, 1 min was enough. The size selectivity in the size range 9.7-2.0 mum was studied as a function of flowrates and particle size and was found to be constant. A particle trapping device made it possible to separate particles of sizes >10 mum; which has previously proven to be difficult in asymmetrical channels. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Analytical Chemistry
volume
74
issue
21
pages
5621 - 5628
publisher
The American Chemical Society
external identifiers
  • wos:000179028300031
  • pmid:12433097
  • scopus:0036828865
ISSN
1520-6882
DOI
10.1021/ac020315s
language
English
LU publication?
yes
id
ea23afda-d41a-480e-9eab-08e91085175c (old id 324336)
date added to LUP
2007-11-09 15:06:28
date last changed
2017-01-01 04:27:40
@article{ea23afda-d41a-480e-9eab-08e91085175c,
  abstract     = {The performance of lift-hyperlayer asymmetrical flow field-flow fractionation using rapid elution conditions was tested through the separation of standard polystyrene latex particles of diameters from 2 to 20 mum. Optimization of flowrates was studied not only in order to obtain efficient and rapid separation; but also to work under conditions of various shape and steepness of the axial flow velocity gradient. Using extreme flow conditions, the five widely spaced particle sizes, 20.5-, 15.0-, 9.7-, 5.0-, and 2.0-mum diameter, could be resolved in 6 min, whereas for the narrower size range of 20.5-5.0 mum, 1 min was enough. The size selectivity in the size range 9.7-2.0 mum was studied as a function of flowrates and particle size and was found to be constant. A particle trapping device made it possible to separate particles of sizes >10 mum; which has previously proven to be difficult in asymmetrical channels.},
  author       = {Wahlund, Karl-Gustav and Zattoni, A},
  issn         = {1520-6882},
  language     = {eng},
  number       = {21},
  pages        = {5621--5628},
  publisher    = {The American Chemical Society},
  series       = {Analytical Chemistry},
  title        = {Size separation of supermicrometer particles in asymmetrical flow field-flow fractionation. Flow conditions for rapid elution},
  url          = {http://dx.doi.org/10.1021/ac020315s},
  volume       = {74},
  year         = {2002},
}