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In vitro molecular evolution of antibody genes mimicking receptor revision

Ellmark, Peter LU ; Esteban, O; Furebring, Christina LU ; Malmborg Hager, Ann-Christin LU and Ohlin, Mats LU (2002) In Molecular Immunology 39(5-6). p.349-356
Abstract
Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective... (More)
Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
phage display, affinity maturation, antibody, receptor revision, somatic hypermutation
in
Molecular Immunology
volume
39
issue
5-6
pages
349 - 356
publisher
Pergamon
external identifiers
  • pmid:12220892
  • wos:000178578300008
  • scopus:0036772983
ISSN
1872-9142
DOI
10.1016/S0161-5890(02)00109-8
language
English
LU publication?
yes
id
e0497862-9d8a-447e-b81d-d9593d16b568 (old id 325515)
date added to LUP
2007-08-17 07:46:34
date last changed
2017-01-01 06:43:52
@article{e0497862-9d8a-447e-b81d-d9593d16b568,
  abstract     = {Antibody evolution in vivo proceeds mainly by stepwise improvements, accomplished by single base pair substitutions. Lately, receptor revision, i.e. exchange of large parts of the V gene for another sequence, has been suggested to provide a complementary route for affinity maturation. By employing a receptor revision like evolution process in vitro using combinatorial libraries and phage display selection, we demonstrate here that maturation of a clone may preferentially proceed through exchange of a large gene segment rather than via minor sequence changes. These modifications of a CD40-specific human antibody fragment outline how receptor revision like events may provide an advantage to a particular clonotype put under selective pressure. (C) 2002 Elsevier Science Ltd. All rights reserved.},
  author       = {Ellmark, Peter and Esteban, O and Furebring, Christina and Malmborg Hager, Ann-Christin and Ohlin, Mats},
  issn         = {1872-9142},
  keyword      = {phage display,affinity maturation,antibody,receptor revision,somatic hypermutation},
  language     = {eng},
  number       = {5-6},
  pages        = {349--356},
  publisher    = {Pergamon},
  series       = {Molecular Immunology},
  title        = {In vitro molecular evolution of antibody genes mimicking receptor revision},
  url          = {http://dx.doi.org/10.1016/S0161-5890(02)00109-8},
  volume       = {39},
  year         = {2002},
}