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Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects

Wisniewska, M; Nilsson, Hans-Olof LU ; Bak-Romaniszyn, L; Rechcinski, T; Bielanski, W; Planeta-Malecka, I; Plonka, M; Konturek, S; Wadström, Torkel LU and Rudnicka, W, et al. (2002) In Microbiology and Immunology 46(10). p.657-665
Abstract
In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not... (More)
In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection. (Less)
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Contribution to journal
publication status
published
subject
keywords
Helicobacter pylori, diagnosis, stool antigens, PCR
in
Microbiology and Immunology
volume
46
issue
10
pages
657 - 665
publisher
Center for Academic Publications Japan
external identifiers
  • pmid:12477244
  • wos:000178508500002
  • scopus:0036033379
ISSN
1348-0421
language
English
LU publication?
yes
id
1bb841cc-7d76-4e71-881e-0d02b0f953bd (old id 325986)
alternative location
http://www.jstage.jst.go.jp/article/mandi/46/10/657/_pdf
date added to LUP
2007-11-19 12:40:34
date last changed
2017-11-19 03:35:07
@article{1bb841cc-7d76-4e71-881e-0d02b0f953bd,
  abstract     = {In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection.},
  author       = {Wisniewska, M and Nilsson, Hans-Olof and Bak-Romaniszyn, L and Rechcinski, T and Bielanski, W and Planeta-Malecka, I and Plonka, M and Konturek, S and Wadström, Torkel and Rudnicka, W and Chmiela, M},
  issn         = {1348-0421},
  keyword      = {Helicobacter pylori,diagnosis,stool antigens,PCR},
  language     = {eng},
  number       = {10},
  pages        = {657--665},
  publisher    = {Center for Academic Publications Japan},
  series       = {Microbiology and Immunology},
  title        = {Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects},
  volume       = {46},
  year         = {2002},
}